EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19835

Gpr182 G protein-coupled receptor 182 ( MGI:109545)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006393. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006393_01	euxassay_006393_02	euxassay_006393_03	euxassay_006393_04

euxassay_006393_05	euxassay_006393_06	euxassay_006393_07	euxassay_006393_08	euxassay_006393_09

euxassay_006393_10	euxassay_006393_11	euxassay_006393_12	euxassay_006393_13	euxassay_006393_14

euxassay_006393_15	euxassay_006393_16	euxassay_006393_17	euxassay_006393_18	euxassay_006393_19

euxassay_006393_20	euxassay_006393_21	euxassay_006393_22	euxassay_006393_23	euxassay_006393_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19835_wholemount_strong.wlz

19835_wholemount_moderate.wlz

19835_wholemount_weak.wlz

19835_wholemount_possible.wlz

19835_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19835_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart ventricle

moderate

spotted

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 weak expression: see section 08

liver

moderate

spotted

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 01 02 03 04 05 06 07 08 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35096
Entity Detected:	Gpr182, G protein-coupled receptor 182 ( MGI:109545)
Sequence:	sense strand is shown >T35096 CTTGTCAACATGTACAGCAGCATCTTCTTCCTGACGTGCCTCAGCATTGACCGCTACGTCACCCTCACCA ACACCTCTCCCTCCTGGCAGCGCCACCAGCACCGAATACGGAGGGCCGTGTGCGCAGGCGTCTGGGTCCT CTCCGCCATTATCCCACTGCCGGAGGTGGTGCACATCCAGCTGTTGGATGGCTCCGAGCCCATGTGCCTC TTCCTAGCACCTTTTGAAACGTACAGCGCCTGGGCCCTGGCAGTGGCCCTGTCGGCCACCATCCTGGGCT TCCTACTGCCTTTTCTTCTCATCGCAGTGTTCAATATCCTGACAGCCTGCCGGCTTCGGAGGCAAAGGCA GACGGAGAGCAGGCGCCACTGCCTGTTGATGTGGGCTTACATAGTTGTCTTTGCCATCTGCTGGCTGCCC TACCAAGTGACTATGCTGCTGCTCACTCTGCACGGGACCCACATCTTCCTCCACTGTCACCTGGTTAACC TTCTCTACTTCTTCTACGAAATCATCGACTGCTTTTCCATGCTGCACTGTGTGGCCAACCCCATCCTCTA CAACTTTCTCAGCCCGAGCTTCCGGGGCCGACTGCTGAGCCTTGTGGTCCGTTACCTTCCCAAGGAGCAG GCCAGGGCAGCAGGTGGTCGAGCCTCCTCTTCTTCTTCCACCCAGCACTCCATCATCATTACCAAAGAGG GCAGCCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 71842. Forward Primer - name:071842_F_cDNA_Admr, sequence:CTTGTCAACATGTACAGCAGCA; Reverse Primer - name:071842_N_SP6_cDNA_Admr, sequence:CAGGCTGCCCTCTTTGGTAAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19837 same embryo
	EMAGE:19840 same embryo
	EMAGE:19839 same embryo
	EMAGE:19838 same embryo
	EMAGE:19836 same embryo
	EurExpress:euxassay_006393 same experiment
	MGI:4825183 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source