Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19873

Raph1 Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 ( MGI:1924550)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19873 EMAGE:19873 EMAGE:19873 EMAGE:19873 EMAGE:19873
"Pseudo-wholemount" of euxassay_006480. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006480_01 euxassay_006480_02 euxassay_006480_03 euxassay_006480_04
EMAGE:19873 EMAGE:19873 EMAGE:19873 EMAGE:19873 EMAGE:19873
euxassay_006480_05 euxassay_006480_06 euxassay_006480_07 euxassay_006480_08 euxassay_006480_09
EMAGE:19873 EMAGE:19873 EMAGE:19873 EMAGE:19873 EMAGE:19873
euxassay_006480_10 euxassay_006480_11 euxassay_006480_12 euxassay_006480_13 euxassay_006480_14
EMAGE:19873 EMAGE:19873 EMAGE:19873 EMAGE:19873 EMAGE:19873
euxassay_006480_15 euxassay_006480_16 euxassay_006480_17 euxassay_006480_18 euxassay_006480_19
EMAGE:19873 EMAGE:19873
euxassay_006480_20 euxassay_006480_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19873Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19873_wholemount_strong.wlz
19873_wholemount_moderate.wlz
19873_wholemount_weak.wlz
19873_wholemount_possible.wlz
19873_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19873_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35781
Entity Detected:Raph1, Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 ( MGI:1924550)
Sequence:sense strand is shown

>T35781
AAAGAACTGAACTGGCCCTGTACAGGTATCACTCACTCATGAAGTTACAATGCAAAGAATCTATTTGGGA
ATGGGATTATCATGAACAAAGTGAGTTTTCATAATTTCATCGATAGCTAGTGGAGCTGCTAAGATCTTTG
CTATTTACAGCACAAGTGTAGAGATTTTGTAGGTAACCAGGTGTCTGATGGAGTAGTGTCACAGTCCAGT
GACAGTGACACTGGCTATGCATAGGCAGCTGGGCAAACCTTGGAAGCATTGCTCCGATGACGCTACTAGA
CTTCACATTCTTCTGTGTGCTTTTGGTACTTTTATCAACTTACAATGACAGGTTGAAGGGAATAAAATAC
AACCTTAGAGCTATTTTTTAAGAGAATAAAAACTGTCCATCCCATCAATTACAAATGAACATATCTTGTA
AAGGAGGTCTGTGCCTGTTGAAAAGATAACCAGTGCCAAACACAAAACTCCTTAGTTCCTGTCCCTCAAG
GAGATAGAAACTGCAGTGAATCTAGGTCAAGACTCATTGGAGAATTTAGTATTGGTACTACTACATCAGA
GAGATGTGTGATTGGCACCTCCTGTCACATTTGCAGCCACTATTCGGTGCAGTCACAGGGAGGAAAAAGA
CCATAGAAGTCATTGTGCTGCTTAAAACCCAACTTAAGGATATTTTTTGAATCTTTCAAATTGTTCACCA
AGTATTTGTGCTTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 75652. Forward Primer - name:075652_F_cDNA_9430025M21Rik, sequence:AAAGAACTGAACTGGCCCTGTA; Reverse Primer - name:075652_N_SP6_cDNA_9430025M21Rik, sequence:GCAAGCACAAATACTTGGTGAAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19877 same embryo
 EMAGE:19874 same embryo
 EMAGE:19876 same embryo
 EMAGE:19875 same embryo
 EurExpress:euxassay_006480 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS