EMAGE mouse gene expression spatial database

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EMAGE:19974

Slc8a1 solute carrier family 8 (sodium/calcium exchanger), member 1 ( MGI:107956)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009439. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009439_01	euxassay_009439_02	euxassay_009439_03	euxassay_009439_04

euxassay_009439_05	euxassay_009439_06	euxassay_009439_07	euxassay_009439_08	euxassay_009439_09

euxassay_009439_10	euxassay_009439_11	euxassay_009439_12	euxassay_009439_13	euxassay_009439_14

euxassay_009439_15	euxassay_009439_16	euxassay_009439_17	euxassay_009439_18	euxassay_009439_19

euxassay_009439_20	euxassay_009439_21	euxassay_009439_22	euxassay_009439_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19974_wholemount_strong.wlz

19974_wholemount_moderate.wlz

19974_wholemount_weak.wlz

19974_wholemount_possible.wlz

19974_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19974_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 23 weak expression: see section 12 13 14 15 16 17 18 19 20 21 22

spinal cord

moderate

regional

moderate expression: see section 09 10 11 weak expression: see section 12 13 14 15 16

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 07 08 09 16 17 18

nasal cavity respiratory epithelium

weak

regional

weak expression: see section 10 11 15

heart atrium

strong

regional

strong expression: see section 06 07 08 09 10 11 moderate expression: see section 12 13 14 15 16 17 18 19 20 21

heart ventricle

strong

regional

strong expression: see section 06 07 08 09 10 11 moderate expression: see section 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36854
Entity Detected:	Slc8a1, solute carrier family 8 (sodium/calcium exchanger), member 1 ( MGI:107956)
Sequence:	sense strand is shown >T36854 CTTCAATTGCTTGTCTTGGGTCACCCAGCACTGCCACCATAACCATTTTTGATAATGACCATACAGGCAT CTTTACATTTGAGGAACCCGTGACTCACGTGAGCGAGAGCATTGGCATCATGGAGGTGAAGGTTTTGAGA ACCTCTGGAGCTCGAGGAAATGTTATCATTCCCTACAAAACTATTGAAGGCACAGCCCGAGGTGGAGGGG AAGACTTTGAGGACACCTGTGGAGTGCCGGAATTCCAGAATGATGAAATAGTCAAAACAATATCAGTCAA GGTAATAATCGATGACGAGGAGTATGAGAAAAACAAGACCTTCATTGAGATTGGAGAGCCCCGTCTGGTG GAGATGAGTGAGAAGAAAGCCCTGTTGTTGAATGAGCTTGGTGGCTTCACATTAACAGGAAAAGAGATGT ATGGCCAACCTATC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101874. Forward Primer - name:101874_F_cDNA_Slc8a1, sequence:CTTCAATTGCTTGTCTTGGGTC; Reverse Primer - name:101874_N_SP6_cDNA_Slc8a1, sequence:GATAGGTTGGCCATACATCTCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19976 same embryo
	EMAGE:19977 same embryo
	EMAGE:19973 same embryo
	EMAGE:19972 same embryo
	EMAGE:19975 same embryo
	EurExpress:euxassay_009439 same experiment
	MGI:4828283 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source