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EMAGE:19989

Sgcd sarcoglycan, delta (dystrophin-associated glycoprotein) ( MGI:1346525)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19989 EMAGE:19989 EMAGE:19989 EMAGE:19989 EMAGE:19989
"Pseudo-wholemount" of euxassay_009461. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009461_01 euxassay_009461_03 euxassay_009461_04 euxassay_009461_05
EMAGE:19989 EMAGE:19989 EMAGE:19989 EMAGE:19989 EMAGE:19989
euxassay_009461_06 euxassay_009461_07 euxassay_009461_08 euxassay_009461_09 euxassay_009461_10
EMAGE:19989 EMAGE:19989 EMAGE:19989 EMAGE:19989 EMAGE:19989
euxassay_009461_11 euxassay_009461_12 euxassay_009461_13 euxassay_009461_14 euxassay_009461_15
EMAGE:19989 EMAGE:19989 EMAGE:19989 EMAGE:19989 EMAGE:19989
euxassay_009461_16 euxassay_009461_17 euxassay_009461_18 euxassay_009461_19 euxassay_009461_20
EMAGE:19989 EMAGE:19989
euxassay_009461_21 euxassay_009461_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19989Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19989_wholemount_strong.wlz
19989_wholemount_moderate.wlz
19989_wholemount_weak.wlz
19989_wholemount_possible.wlz
19989_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19989_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
mandible
weak weak
regionalweak expression: see section 01 03 04 05 06 07 09 15 16 17 19 20 21
maxilla
weak weak
regionalweak expression: see section 01 03 04 05 06 07 09 16 17 18 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37012
Entity Detected:Sgcd, sarcoglycan, delta (dystrophin-associated glycoprotein) ( MGI:1346525)
Sequence:sense strand is shown

>T37012
CAGGAACGTGACAGTGAACATTCTCAATGATCAGACAAAAGTGCTGACTCAGCTGGTGACAGGTCCGAAG
GCTGTGGAAGCATATGGCAAAAGGTTTGAAGTAAAAACTGTTTCTGGAAAATTGCTCTTTTCTGCGGATG
ACAGTGAAGTGGTCGTGGGAGCTGAGAGATTGAGAGTCTTAGGAGCTGAAGGCACAGTCTTCCCCAAATC
CATAGAAACACCTAATGTCAGAGCAGACCCCTTCAAGGAACTAAGGTTGGAGTCCCCAACCCGATCTCTA
GTGATGGAAGCCCCAAAAGGAGTAGAAATAAATGCAGAAGCTGGCAATATGGAAGCCATCTGCAGAAGTG
AGCTGAGACTGGAGTCCAAGGATGGAGAGATTAAGTTAGATGCTGCGAAAATCAAACTACCTAGACTGCC
TCGTGGATCCTACACACCCACAGGAACGAGGCAGAAGGTCTTCGAGGTCTGCGTCTGCGCCAATGGGAGG
TTATTCCTGTCCCAGGCAGGAACTGGTTCTACCTGTCAGATAAACACAAGTGTCTGCCTTTGAAAGACTA
TCCATAGTGGACAATTGCCGGCAGCATAAAGGCCCTTTTTGGCTTGAAACACTGGCTGCCAGCTATTTTT
ACTAGAACACAGAAAGCCTATCAAAGCCCTTTTGTGTGTATGTGTATG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95379. Forward Primer - name:095379_F_cDNA_Sgcd, sequence:CAGGAACGTGACAGTGAACATT; Reverse Primer - name:095379_N_SP6_cDNA_Sgcd, sequence:CATACACATACACACAAAAGGGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19992 same embryo
 EMAGE:19991 same embryo
 EMAGE:19988 same embryo
 EMAGE:19990 same embryo
 EurExpress:euxassay_009461 same experiment
 MGI:4828021 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS