EMAGE mouse gene expression spatial database

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EMAGE:20051

C920008G01Rik RIKEN cDNA C920008G01 gene ( MGI:2444626)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012168. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012168_01	euxassay_012168_02	euxassay_012168_03	euxassay_012168_04

euxassay_012168_05	euxassay_012168_06	euxassay_012168_07	euxassay_012168_08	euxassay_012168_09

euxassay_012168_10	euxassay_012168_11	euxassay_012168_12	euxassay_012168_13	euxassay_012168_14

euxassay_012168_15	euxassay_012168_16	euxassay_012168_17	euxassay_012168_18	euxassay_012168_19

euxassay_012168_20	euxassay_012168_21	euxassay_012168_22	euxassay_012168_23	euxassay_012168_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20051_wholemount_strong.wlz

20051_wholemount_moderate.wlz

20051_wholemount_weak.wlz

20051_wholemount_possible.wlz

20051_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20051_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper leg muscle

moderate

spotted

moderate expression: see section 03 23 24

diaphragm

moderate

spotted

moderate expression: see section 13 14

vertebral axis musculature

moderate

spotted

moderate expression: see section 01 02 05 06 07 08 09 12 13 14 15 16 17 18 19 20 22 23 24

pituitary gland

moderate

spotted

moderate expression: see section 12 13 14 15

heart left ventricle

moderate

spotted

moderate expression: see section 06 07 08 09 10

heart right ventricle

moderate

spotted

moderate expression: see section 09 10

tongue

moderate

spotted

moderate expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37616
Entity Detected:	C920008G01Rik, RIKEN cDNA C920008G01 gene ( MGI:2444626)
Sequence:	sense strand is shown >T37616 CAACAGTGCTTCTCAGTCCATCTGGTGGTACCAACAGCATCCTGGGAAAGGCCCCGAAGCACTAATATCC ATATTCTCTAATGGCAACAAGAAAGAAGGCAGATTGACAGTTTACCTCAATAGAGCCAGCCTGCATGTTT CCCTGCACATCAGAGACTCCCAGCCCAGTGACTCTGCCGTCTACCTCTGTGCAGTGAGCACACAGTGCTC CCCAGGCACCTGCAGCCTGCACCCAAACATAGTGGGCTTCCGCAGGTCTGAGCTGGAGTTCAGAACATAA GGGGGAGAAATCCTGCTTATTCTCTTTGTCCTCCACATGTAATGGGAGTTGAGAGCCACTCAAGAGAGGG TTCGACTTATGGGGATATTACTTTATTATCCTGAGAATGCATAGTACACATATTGGTATGGCTGAAATAT TTTTATTTTATTTTAATTGAAAAAATGCATACGCTATATCCTGATCATGGTTTGCTCTCCTCCAATTCCT TCCATATTCTCCCCCACCTCCCCATACAACGACTACTATACCTCCTTTCTCTATATATCTTTAGAAAACA ATCAGGCATATAAAAAAATTGAAAGAAAGAAAAGGCACAAGCTACACACATATGAGCCATTTGGGACCAG GACATGACATTCCTATCCTAGCTCCCTGGCTGCAGCCCCTCCCAGCAAACCCAGCAGTGGTGAGAGCACA TTCTGACTTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 69812. Forward Primer - name:069812_F_cDNA_C920008G01Rik, sequence:CAACAGTGCTTCTCAGTCCATC; Reverse Primer - name:069812_N_SP6_cDNA_C920008G01Rik, sequence:GAAGTCAGAATGTGCTCTCACC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20049 same embryo
	EMAGE:20052 same embryo
	EMAGE:20053 same embryo
	EMAGE:20050 same embryo
	EMAGE:20054 same embryo
	EurExpress:euxassay_012168 same experiment
	MGI:4823567 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source