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EMAGE:20059

Rec8 REC8 homolog (yeast) ( MGI:1929645)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20059 EMAGE:20059 EMAGE:20059 EMAGE:20059 EMAGE:20059
"Pseudo-wholemount" of euxassay_012260. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012260_01 euxassay_012260_02 euxassay_012260_03 euxassay_012260_04
EMAGE:20059 EMAGE:20059 EMAGE:20059 EMAGE:20059 EMAGE:20059
euxassay_012260_05 euxassay_012260_06 euxassay_012260_07 euxassay_012260_08 euxassay_012260_09
EMAGE:20059 EMAGE:20059 EMAGE:20059 EMAGE:20059 EMAGE:20059
euxassay_012260_10 euxassay_012260_11 euxassay_012260_12 euxassay_012260_13 euxassay_012260_14
EMAGE:20059 EMAGE:20059 EMAGE:20059 EMAGE:20059 EMAGE:20059
euxassay_012260_15 euxassay_012260_16 euxassay_012260_17 euxassay_012260_18 euxassay_012260_19
EMAGE:20059
euxassay_012260_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20059Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20059_wholemount_strong.wlz
20059_wholemount_moderate.wlz
20059_wholemount_weak.wlz
20059_wholemount_possible.wlz
20059_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20059_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
pancreas
weak weak
regionalweak expression: see section 08 09
thyroid gland
weak weak
regionalweak expression: see section 08 12
pituitary gland
weak weak
regionalweak expression: see section 08 09 10 11 12 13
hypothalamus mantle layer
weak weak
regionalweak expression: see section 09 12 13
hypothalamus ventricular layer
weak weak
regionalweak expression: see section 10 11
cerebral cortex mantle layer
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20
trigeminal v ganglion
weak weak
regionalweak expression: see section 07 13 14
ventral grey horn
weak weak
regionalweak expression: see section 08 12
cochlea
weak weak
regionalweak expression: see section 03 04 15 16 17
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 07 14 15 16 moderate expression: see section 08 09 12 13 weak expression: see section 06
stomach
strong strong
regionalstrong expression: see section 02 moderate expression: see section 03 04 05 weak expression: see section 06 07
metanephros
moderate moderate
regionalmoderate expression: see section 07 08 14 15 weak expression: see section 06 09
ovary
strong strong
regionalstrong expression: see section 05 18 moderate expression: see section 06 17
urethra of male
moderate moderate
regionalmoderate expression: see section 11 12 13
lung
strong strong
regionalstrong expression: see section 07 13 14 15 18 19 moderate expression: see section 03 04 05 06 08 12 16 17 weak expression: see section 09
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30394
Entity Detected:Rec8, REC8 homolog (yeast) ( MGI:1929645)
Sequence:sense strand is shown

>T30394
CCTGGCCATGATGGAGACGCTGGAAGATGCCCCAGAACCCTTTTTTGGGAAGATGTCTGTGGATCCTAGA
CTTCCCAGTCCTTTTGATATTCCTCAGATTCGACACCTTTTAGAGGCTGCAACCCCGGAAAAAACTCGTA
AGGAGACCCTTCCTGAAGCCACTCCAGACCCCAGGAAGCCTGACAGGACCTTGGCCACCGTGCAGTCCCC
TGAGGTCATCACCTTACAGGAGGCAGAGCCCATCCGCATGCTGCAGATTGAAGGAGAGCAGGATCTCCCA
GAGATCAGTCGAGGAGACTTAGAACTGCTGATCGCAGAGAAAGATGACGCCATCTTGCTAGAGGAACGTC
AGAGAGGCAGGCTTCTCCGACAGCGGAGAGCTTCACTACCACTGGATGAATCCAGGGAAGAACCCCGGGC
CCTAGAAGGTGCTGGTTTGGTCTCGGCACTCTCACCTCCAGCTCCTGCGCAGGTGGAAGGGATACAAGAA
GCACTACCAGGCCAGGTCTTCCCTCCTGAGGTACAGAAGATGACAGGCTGGGAGCCTGGGGCCCTACTCA
CTGAGGTGACCCCACCTCAGGAGCTGCGGCTGCCTGCCCCACCCAGTACAGAGAAGAGGCTCCCGTCTCT
TCAGAGGCCACTTCCCCGCCGACACCGGCGGCGCCAGTTATTATTCTGGGACAAGGAGACCCAGATCTCT
CGGGAGAAGTTTGAGGAACAACTGCAGACTGGGGCTCACTGCTGGGAGTATCCAGTGGCCCAGCCTCCCA
AGAGGATGCTCACAAGCCCAGCGGAGCTCTTCAGAACCCCAACTCTCTCTGGCTGGCTACCCCCAGAACT
CCTAGGTTTATGGACACACTGTGCCCAGGTCCCCCAAAGAATGCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6335959), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58530. Forward Primer - name:058530_F_IRAV103_a11_Rec8L1, sequence:CCTGGCCATGATGGAGAC; Reverse Primer - name:058530_R_SP6_IRAV103_a11_Rec8L1, sequence:TGAGCATTCTTTGGGGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20057 same embryo
 EMAGE:20058 same embryo
 EMAGE:20056 same embryo
 EMAGE:20055 same embryo
 EurExpress:euxassay_012260 same experiment
 MGI:4827677 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS