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EMAGE:20067

Cdk13 cyclin-dependent kinase 13 ( MGI:1916812)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20067 EMAGE:20067 EMAGE:20067 EMAGE:20067 EMAGE:20067
"Pseudo-wholemount" of euxassay_012175. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012175_01 euxassay_012175_02 euxassay_012175_03 euxassay_012175_04
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euxassay_012175_05 euxassay_012175_06 euxassay_012175_07 euxassay_012175_08 euxassay_012175_09
EMAGE:20067 EMAGE:20067 EMAGE:20067 EMAGE:20067 EMAGE:20067
euxassay_012175_10 euxassay_012175_11 euxassay_012175_12 euxassay_012175_13 euxassay_012175_14
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euxassay_012175_15 euxassay_012175_16 euxassay_012175_17 euxassay_012175_18 euxassay_012175_19
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euxassay_012175_20 euxassay_012175_21 euxassay_012175_22 euxassay_012175_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20067Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20067_wholemount_strong.wlz
20067_wholemount_moderate.wlz
20067_wholemount_weak.wlz
20067_wholemount_possible.wlz
20067_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20067_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37625
Entity Detected:Cdk13, cyclin-dependent kinase 13 ( MGI:1916812)
Sequence:sense strand is shown

>T37625
GGGCTTAGATGACAGCAGAACTAACACACCCCAGGGTGTGCTGCCACCCGCACAGTTGAAATCTCAGAGC
AACTCAAATGTAGCACCTGTAATAACAGGCCCTGGACAGCCGTTAAACCACAGTGAATTGGCAATTCTTC
TAAACCTACTACAATCTAAATCAAGTGTTAATATGGCTGATTTTGTCCAAGTGTTGAACATTAAGGTAAA
CTCTGAGACTCAACAGCAGCTAAATAAAATAAACCTTCCTGCTGGAATTTTGGCAACAGGTGAAAAACAG
ACAGATCCATCAACACCACAACAGGAGTCTTCAAAATCATTGGGAGGAGTTCAGCCTTCACAGACCATCC
AGCCTAAAGTGGAAACTGATGCTGCCCAGGCTGCTGTGCAGAGTGCATTTGCAGTCCTCTTGACTCAGTT
AATAAAGGCCCAACAGTCCAAACAGAAAGATGCCATGCTAGAGGAAAGGGAAAATGGATCAGGACATGAA
GCTCCATTGCAACTCAGGCCTCCTCTAGAACCGAGCACTCCTGGATCCGGGCAAGATGACCTCATCCAGC
ACCAAGACAGGAGGATATTGGAGCTGACACCAGAACCAGACCGGCCTCGAATTCTGCCTCCTGATCAACG
GCCTCCTGAACCTCCTGAACCACCACCAGTTACTGAGGAGGACCTAGATTATCGGACAGAAAACCAGCAT
GTTCCTACCACTAGTTCTTCATTAACTGACCCACATGCTGGAGTGAAGGCAGCCCTCTTACAGCTGCTTG
CTCAGCATC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 84071. Forward Primer - name:084071_F_cDNA_Cdc2l5, sequence:GGGCTTAGATGACAGCAGAACT; Reverse Primer - name:084071_N_SP6_cDNA_Cdc2l5, sequence:GATGCTGAGCAAGCAGCTGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20068 same embryo
 EMAGE:20066 same embryo
 EMAGE:20071 same embryo
 EMAGE:20070 same embryo
 EMAGE:20069 same embryo
 EurExpress:euxassay_012175 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS