EMAGE mouse gene expression spatial database

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EMAGE:20099

D630042F21Rik RIKEN cDNA D630042F21 gene ( MGI:2442838)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012221. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012221_01	euxassay_012221_02	euxassay_012221_03	euxassay_012221_04

euxassay_012221_05	euxassay_012221_06	euxassay_012221_07	euxassay_012221_08	euxassay_012221_09

euxassay_012221_10	euxassay_012221_11	euxassay_012221_12	euxassay_012221_13	euxassay_012221_14

euxassay_012221_15	euxassay_012221_16	euxassay_012221_17	euxassay_012221_18	euxassay_012221_19

euxassay_012221_20	euxassay_012221_21	euxassay_012221_22	euxassay_012221_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20099_wholemount_strong.wlz

20099_wholemount_moderate.wlz

20099_wholemount_weak.wlz

20099_wholemount_possible.wlz

20099_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20099_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

metanephros

strong

regional

strong expression: see section 08 09 16 18 19 moderate expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37659
Entity Detected:	D630042F21Rik, RIKEN cDNA D630042F21 gene ( MGI:2442838)
Sequence:	sense strand is shown >T37659 GGTGGGTTCTCACTCTTCATTCACAACAACGTGCTTTTTCTGATACACACATTGCTAATTGAGTTCTAAC TCGTTATTCAAAGTTCAAAGCTTGCAACTCACCTTTAGCTATATTGACTACTACCAAAGCATGGAACATT AAGGTGTCTTGAATTCCTTCAACTACAGCATGCATATAAAGTTTCTTTAACCCTATAATCCTGCAAATTG CTCTTCCCTCTACTTGACTGTGGTAATCTTGAAGGGAGACATACTTTGTGCACAGCCACTACCATCAGTG CACCAAATCCATGCATCTTTCCTTTTAGGGAACAACCACCATTATGCTGAACAACCTGGTACACCATAGA ATCCCCACCTGGACTTGCAGATTGCACAGCCAGGGGTTCTGATATGAGTGGAGAGACTATACTACAGTAT GTGGCACATGTCAAAACCGTTCTATCAGTTTAGTCTTACCCACAAGAGTGAAACATTGTATAGAGTACAG AAGTGGTCCTAGCAACTTTTCAGTGCTTTGTCCTGCAAACACACACACACACACTCACACACACACACAC ACACACACTTTTTAGGCTCTGAAAGAAAGCATTCCTCTGCTTCTTTCCTTCTTGTTCTCAGGATGGAAAG CCTTCACCATGTAGTCTAGAGTGACCTATCCTGGCTCTTGAGGTGGGTCTTGCTGCCACCTAGTGGAACA ATAATTAAAAATTCACTCCGTTGAGGTCTGTGCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 79888. Forward Primer - name:079888_F_cDNA_D630042F21Rik, sequence:GGTGGGTTCTCACTCTTCATTC; Reverse Primer - name:079888_N_SP6_cDNA_D630042F21Rik, sequence:GTGCACAGACCTCAACGGAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20097 same embryo
	EMAGE:20098 same embryo
	EMAGE:20100 same embryo
	EMAGE:20101 same embryo
	EurExpress:euxassay_012221 same experiment
	MGI:4824206 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source