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EMAGE:20106

Dhrs2 dehydrogenase/reductase member 2 ( MGI:1918662)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20106 EMAGE:20106 EMAGE:20106 EMAGE:20106 EMAGE:20106
"Pseudo-wholemount" of euxassay_012319. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012319_01 euxassay_012319_02 euxassay_012319_03 euxassay_012319_04
EMAGE:20106 EMAGE:20106 EMAGE:20106 EMAGE:20106 EMAGE:20106
euxassay_012319_05 euxassay_012319_06 euxassay_012319_07 euxassay_012319_08 euxassay_012319_09
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euxassay_012319_10 euxassay_012319_11 euxassay_012319_12 euxassay_012319_13 euxassay_012319_14
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euxassay_012319_15 euxassay_012319_16 euxassay_012319_17 euxassay_012319_18 euxassay_012319_19
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euxassay_012319_20 euxassay_012319_21 euxassay_012319_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20106Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20106_wholemount_strong.wlz
20106_wholemount_moderate.wlz
20106_wholemount_weak.wlz
20106_wholemount_possible.wlz
20106_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20106_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
lower lip
strong strong
regionalstrong expression: see section 07 08 15 17 18 moderate expression: see section 06
upper lip
strong strong
regionalstrong expression: see section 07 08 17 moderate expression: see section 06
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37672
Entity Detected:Dhrs2, dehydrogenase/reductase member 2 ( MGI:1918662)
Sequence:sense strand is shown

>T37672
TGTGTCTACCTCTCTCAGCTCGAAGGTTCAGCAAGACAGCGGATGAGAATCGCTCCCTGGCTGGGAAAGT
GGCTGTGATCACTGGTTCCACGAGAGGGATTGGCTTTGCCATCGCCAGGCGTCTGGCCCAGGATGGGGCA
CATGTGGTCATCAGCAGCCGGAAGCAGGAGAATGTAGATGAAGCTGTGACCATATTGAAGGAGGAGGGGC
TGAGTGTGACTGGCACCATGTGCCACGTGGGGAAGGCTGAGGACCGCCAGCACCTTGTGACCACAGCCCT
GAAGCATTCTGGGGGCATTGACTTCCTTGTGTGTGTGGCCGGAGTCAACCCTCTGGTGGGAAGCACCCTG
GGAGCCAGTGAACAGATCTGGGACAAGATCCTGGATGTAAATGTGAAGTCTCCAGCTCTGTTGCTAAGCA
AGGTGCTGCCCTACATGGAGAACAGAAGGGGAGGCAGCGTTGTTCTGGTGTCCTCCGGAGTGGCTTATGT
ACCAGTTCCAAAACTGGGCGTCTACAACACCAGCAAGACCGCTCTGTTGGGGCTCTGCAAGTCTCTGGCT
GTGGAGCTGGCTCCAAAGGGCATCAGAGTCAACTGCCTAGTGCCAGGAATTA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 65470. Forward Primer - name:065470_F_cDNA_Dhrs2, sequence:TGTGTCTACCTCTCTCAGCTCG; Reverse Primer - name:065470_N_SP6_cDNA_Dhrs2, sequence:TAATTCCTGGCACTAGGCAGTTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20108 same embryo
 EMAGE:20111 same embryo
 EMAGE:20110 same embryo
 EMAGE:20107 same embryo
 EMAGE:20109 same embryo
 EurExpress:euxassay_012319 same experiment
 MGI:4824299 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS