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EMAGE:20163

Tlr13 toll-like receptor 13 ( MGI:3045213)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20163 EMAGE:20163 EMAGE:20163 EMAGE:20163 EMAGE:20163
"Pseudo-wholemount" of euxassay_012413. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012413_01 euxassay_012413_02 euxassay_012413_03 euxassay_012413_04
EMAGE:20163 EMAGE:20163 EMAGE:20163 EMAGE:20163 EMAGE:20163
euxassay_012413_05 euxassay_012413_06 euxassay_012413_07 euxassay_012413_08 euxassay_012413_09
EMAGE:20163 EMAGE:20163 EMAGE:20163 EMAGE:20163 EMAGE:20163
euxassay_012413_10 euxassay_012413_11 euxassay_012413_12 euxassay_012413_13 euxassay_012413_14
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euxassay_012413_15 euxassay_012413_16 euxassay_012413_17 euxassay_012413_18 euxassay_012413_19
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euxassay_012413_20 euxassay_012413_21 euxassay_012413_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20163Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20163_wholemount_strong.wlz
20163_wholemount_moderate.wlz
20163_wholemount_weak.wlz
20163_wholemount_possible.wlz
20163_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20163_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
vibrissa
weak weak
regionalweak expression: see section 04 05
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38055
Entity Detected:Tlr13, toll-like receptor 13 ( MGI:3045213)
Sequence:sense strand is shown

>T38055
GAGGCATGGGTATACAAAGAGCTTGTTCCAGCCCTAGAACAAGGCAGCCAGACCACCTTTAAACTCTGTC
TTCACCAACGGGATTTTGAACCAGGCATTGATATCTTCGAGAACATCCAGAATGCTATTAACACAAGCAG
GAAAACCTTGTGCGTAGTCAGTAACCACTACCTACACAGTGAATGGTGCCGACTTGAAGTCCAGCTGGCT
AGCATGAAGATGTTTTATGAGCACAAGGATGTCATTATCTTGATCTTCCTTGAAGAGATTCCGAACTATA
AGCTGTCCAGCTACCACCGACTCAGGAAACTCATAAACAAACAGACGTTTATCACTTGGCCGGACAGTGT
TCACCAGCAGCCACTTTTCTGGGCTCGCATCAGAAATGCGTTGGGCAAGGAGACTGTGGAGAAAGAAAAT
ACACATCTAATTGTTGTTGAGTGACTCGAGTCTTAACAATCCCAAACCCGGCTGCATGAATGTTGCCCAA
AATAAATGTAAGCTGTTTGACTAAATGAACTCTCTCTCTCTCTCCAGAGACAAACATGATTGCTTAGATA
GTGTTCATCAGAAGAGTAGGGAGGACTGCTTGGAAAACTTTCAGGAGCAGGGTCCCACTATGATGGGATT
GGATATGCAGAAGGAATTCCTATTTTATTAAGGAGAGGACTTAGAACAACAGTCATCTGAACACTGAGGA
ACAGGAAGGTCTTCGCATATATTCGATATCTTTGCTCATGTTGTTTCTCCAAATCAGTTGTGGTCATGGG
AGACTGCACTTGTGTATCCAAATTGCCCCAAGATTGAAACTTTCCATCTTGAAAGGAAACTTCCTATGAC
AGAGGTTTACAGGGAGAGAGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96734. Forward Primer - name:096734_F_cDNA_Tlr13, sequence:GAGGCATGGGTATACAAAGAGC; Reverse Primer - name:096734_N_SP6_cDNA_Tlr13, sequence:TCTCTCTCCCTGTAAACCTCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20164 same embryo
 EMAGE:20160 same embryo
 EMAGE:20162 same embryo
 EMAGE:20161 same embryo
 EMAGE:20165 same embryo
 EurExpress:euxassay_012413 same experiment
 MGI:4828741 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS