EMAGE mouse gene expression spatial database

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EMAGE:20168

Cyb5r4 cytochrome b5 reductase 4 ( MGI:2386848)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012487. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012487_01	euxassay_012487_02	euxassay_012487_03	euxassay_012487_04

euxassay_012487_05	euxassay_012487_06	euxassay_012487_07	euxassay_012487_08	euxassay_012487_09

euxassay_012487_10	euxassay_012487_11	euxassay_012487_12	euxassay_012487_13	euxassay_012487_14

euxassay_012487_15	euxassay_012487_16	euxassay_012487_17	euxassay_012487_18	euxassay_012487_19

euxassay_012487_20	euxassay_012487_21	euxassay_012487_22	euxassay_012487_23	euxassay_012487_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20168_wholemount_strong.wlz

20168_wholemount_moderate.wlz

20168_wholemount_weak.wlz

20168_wholemount_possible.wlz

20168_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20168_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 11 12 13 14 15

submandibular gland primordium

weak

regional

weak expression: see section 05 06 07 08 15 16 17 18

pituitary gland

weak

regional

weak expression: see section 10 11 12 13 14 15

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 10 11 17 18

facial vii ganglion

weak

regional

weak expression: see section 05 06

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 17 18 19 20 21 22 23

ventral grey horn

weak

regional

weak expression: see section 12 13 16 17

dorsal root ganglion

weak

regional

weak expression: see section 10 11 12 13 17 18

neural retina

weak

regional

weak expression: see section 01 21 22 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

metanephros

moderate

regional

moderate expression: see section 08 weak expression: see section 09 10 11 12 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31369
Entity Detected:	Cyb5r4, cytochrome b5 reductase 4 ( MGI:2386848)
Sequence:	sense strand is shown >T31369 ATGGCATGCTTCCCAAGAGCCAAATGTCAGACACACTGCCCAGGGATGTGACAGACACACTGCCCAGGGA TGTGACAGACACACTGCCCAGGGAAGACCTCAGTTCTCCAAGCTATGATTGGTTCCAAACAGAGTCTTCA GTCACCATTGTTGTATACACCAAACAAAAGAACATCAGTTTAGACTCAGTAATCGTTGACCTCCAGGATG ACTCCTTGAGAGCAGAAGCAATCATCAAGGATCACTCTTACCTTGTACATGTTGGGCTAAGCCATGAAGT TCAAGAAAATTTTTCTGTACGGGTTATTGAAAATGTTGGAAAAATAGAGATTGTCCTACAAAAGAAAGAA AGTGTTTCTTGGCAATGTCTTGGTGATCACCTGGAGAAACATGATTCTTTCATTCCAAAGAAAGATACAG GCTTGTACTACAGACGGTGCCAGTTAATATCTAAGGAAGATGTCACTCATGACACGAGACTTTTCTGTCT GATGCTGCCTCCTAGTACTCACCTTCAGGTGCCAGTTGGACAACACGTTTACCTCAAGCTCAGTGTCACA GGTGCAGAAATAGTAAAGCCATATACACCCGTGTCTGACTCCTTACTCTCAGATTTTAAAGAACCAGTTC TTTCCCCAAATAAATACATCTACTTTTTGATCAAAATCTATCCTGCTGGACTCTTCACACCAGAGCTTGA TCGTCTTCAGATTGGAGATTTTATTTCTGTAAGCGGACCTGAAGGCAATTTTAAAGTATCCACACTCCAG GAAGTAGAAGATCTCTTTCTATTGGCAGCCGGAACAGGATTCACACCAATGGTCACTGTACTGAATTATG CTTTGTCTCACATGTCCAGTCTCAGGAAGGTGAAGTTGATGTTCTTCAATAAAACAGAGGACGATATAAT TTGGAGATGTCAACTAGAGAAGTTGGCATTAAGAGAAAAAAGATTTGATGTTGAATTTGTTCTCTCGGCA CCTTCTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5038563), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 35734. Forward Primer - name:035734_F_IRAV55-58_D17_Ncb5or, sequence:ATGGCATGCTTCCCAAGA; Reverse Primer - name:035734_R_SP6_IRAV55-58_D17_Ncb5or, sequence:TGGAGAAGGTGCCGAGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20172 same embryo
	EMAGE:20169 same embryo
	EMAGE:20171 same embryo
	EMAGE:20170 same embryo
	EMAGE:20173 same embryo
	EurExpress:euxassay_012487 same experiment
	MGI:4824146 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source