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EMAGE:20223

2700007P21Rik RIKEN cDNA 2700007P21 gene ( MGI:1926020)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20223 EMAGE:20223 EMAGE:20223 EMAGE:20223 EMAGE:20223
"Pseudo-wholemount" of euxassay_012294. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012294_01 euxassay_012294_02 euxassay_012294_03 euxassay_012294_04
EMAGE:20223 EMAGE:20223 EMAGE:20223 EMAGE:20223 EMAGE:20223
euxassay_012294_05 euxassay_012294_06 euxassay_012294_07 euxassay_012294_08 euxassay_012294_09
EMAGE:20223 EMAGE:20223 EMAGE:20223 EMAGE:20223 EMAGE:20223
euxassay_012294_10 euxassay_012294_11 euxassay_012294_12 euxassay_012294_13 euxassay_012294_14
EMAGE:20223 EMAGE:20223 EMAGE:20223 EMAGE:20223 EMAGE:20223
euxassay_012294_15 euxassay_012294_16 euxassay_012294_17 euxassay_012294_18 euxassay_012294_19
EMAGE:20223 EMAGE:20223
euxassay_012294_20 euxassay_012294_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20223Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20223_wholemount_strong.wlz
20223_wholemount_moderate.wlz
20223_wholemount_weak.wlz
20223_wholemount_possible.wlz
20223_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20223_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
olfactory cortex ventricular layer
weak weak
homogeneousweak expression: see section 06 07 11 12
telencephalon ventricular layer
weak weak
homogeneousweak expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 06 07 08 10 11 12 weak expression: see section 05 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30454
Entity Detected:2700007P21Rik, RIKEN cDNA 2700007P21 gene ( MGI:1926020)
Sequence:sense strand is shown

>T30454
GACGATGGATCCGTGTTCAGTTGGAGTCCAGCTTCGTACCACACACGACTGCCATAAAACCTTCTATACT
CGGCACACAGGTTTCAAGACATTGAAAGAATTGTCATCAAATGACATGCTTTTACTTCAGCTTAGAACTG
GAATGACACTTTCTGGGAATAATACAATTTGTCTCCATCACGTAAAAATTTATATTGACAGATTTGAAGA
TTTGCGGAAGTCGTGTTGTGACCCATTTAACATACACAAGAAGCTAGCCAAAAAGAACCTGCACGTCATT
GACTTAGATGATGCCACTTTTCTGAGCGCCAAGTTTGGAAGACAGCTTGTACCTGGTTGGAAGCTTTGCC
CCAAATGTACCCAGATCATCAATGGAAGTGTGGACGTTGATTCAGATGACCGCCAGAGACGGAAACCCGA
CTCAGATGGAAGGACTGCTAAAGCTCTGAGGTCACTACAGTTTACAAACCCAGGGAAGCAAACTGAATTT
GCTCCAGAAGGCGGTAAGAGGGAGAAAAGAAAGCTCACCAAAGCCACA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30021622), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58898. Forward Primer - name:058898_F_IRAV106_c01_2700007P21Rik, sequence:GACGATGGATCCGTGTTCA; Reverse Primer - name:058898_R_SP6_IRAV106_c01_2700007P21Rik, sequence:TTGTGGCTTTGGTGAGCTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20221 same embryo
 EMAGE:20226 same embryo
 EMAGE:20222 same embryo
 EMAGE:20225 same embryo
 EMAGE:20224 same embryo
 EurExpress:euxassay_012294 same experiment
 MGI:4822684 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS