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EMAGE:20293

Gigyf2 GRB10 interacting GYF protein 2 ( MGI:2138584)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20293 EMAGE:20293 EMAGE:20293 EMAGE:20293 EMAGE:20293
"Pseudo-wholemount" of euxassay_012648. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012648_01 euxassay_012648_02 euxassay_012648_03 euxassay_012648_04
EMAGE:20293 EMAGE:20293 EMAGE:20293 EMAGE:20293 EMAGE:20293
euxassay_012648_05 euxassay_012648_06 euxassay_012648_07 euxassay_012648_08 euxassay_012648_09
EMAGE:20293 EMAGE:20293 EMAGE:20293 EMAGE:20293 EMAGE:20293
euxassay_012648_10 euxassay_012648_11 euxassay_012648_12 euxassay_012648_13 euxassay_012648_14
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euxassay_012648_15 euxassay_012648_16 euxassay_012648_17 euxassay_012648_18 euxassay_012648_19
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euxassay_012648_20 euxassay_012648_21 euxassay_012648_22 euxassay_012648_23 euxassay_012648_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20293Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20293_wholemount_strong.wlz
20293_wholemount_moderate.wlz
20293_wholemount_weak.wlz
20293_wholemount_possible.wlz
20293_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20293_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31538
Entity Detected:Gigyf2, GRB10 interacting GYF protein 2 ( MGI:2138584)
Sequence:sense strand is shown

>T31538
TCAGTCAACGCCTCGTCAGAGCGACTGAACATGGGTGAGATTGAGACTCTGGATGACTACTGAGTTCCTG
CCGCTGTGCCAGCCTTCCCACTCCCGCCTAGTGACCATGGTTTCTCCTCATTGGACACAACATACGTTCA
CCATTTTCTCTGTCACTCTGCAACAAATCACAGAACCAATCATCTCAGGCTTTTTTTTTCCTAGCCCTTT
GTGTCCAAGATTACTTTAGATCACTGTTGGTGAGCATCTCAGACTGTACCTAGTGGACCAGCCCTTGTCT
CAGGGTGGCAGTTGGGGCTGCTCCCCAGCAGAGCTAATGTTGCCAGCACACATTCACTGTGCGTTGATTT
CATCTACTGTCTCCTGGAAGTGTGTTGGAATCTGCCAATAGCAGATCACTTTCTCTTATCACTTTTCTTG
TTCTGTTTTGTTCTTTCTCCCCACCCCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5357184), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 62839. Forward Primer - name:062839_F_IRAV67-70_I01_Tnrc15, sequence:TCAGTCAACGCCTCGTCA; Reverse Primer - name:062839_R_SP6_IRAV67-70_I01_Tnrc15, sequence:GAGGGGTGGGGAGAAAGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20295 same embryo
 EMAGE:20298 same embryo
 EMAGE:20296 same embryo
 EMAGE:20297 same embryo
 EMAGE:20294 same embryo
 EurExpress:euxassay_012648 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS