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EMAGE:20303

Ube3b ubiquitin protein ligase E3B ( MGI:1891295)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20303 EMAGE:20303 EMAGE:20303 EMAGE:20303 EMAGE:20303
"Pseudo-wholemount" of euxassay_012563. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012563_01 euxassay_012563_02 euxassay_012563_03 euxassay_012563_04
EMAGE:20303 EMAGE:20303 EMAGE:20303 EMAGE:20303 EMAGE:20303
euxassay_012563_05 euxassay_012563_06 euxassay_012563_07 euxassay_012563_08 euxassay_012563_09
EMAGE:20303 EMAGE:20303 EMAGE:20303 EMAGE:20303 EMAGE:20303
euxassay_012563_10 euxassay_012563_11 euxassay_012563_12 euxassay_012563_13 euxassay_012563_14
EMAGE:20303 EMAGE:20303 EMAGE:20303 EMAGE:20303 EMAGE:20303
euxassay_012563_15 euxassay_012563_16 euxassay_012563_17 euxassay_012563_18 euxassay_012563_19
EMAGE:20303 EMAGE:20303 EMAGE:20303
euxassay_012563_20 euxassay_012563_21 euxassay_012563_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20303Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20303_wholemount_strong.wlz
20303_wholemount_moderate.wlz
20303_wholemount_weak.wlz
20303_wholemount_possible.wlz
20303_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20303_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31429
Entity Detected:Ube3b, ubiquitin protein ligase E3B ( MGI:1891295)
Sequence:sense strand is shown

>T31429
ACAGCTCCGTGGATGAGCTTCCCTCCCTGGACTCGGAGTTTTATAAAAACCTCACCTCCATCAAGCGCTA
CGATGGAGACATCGCAGACCTCGGGCTGACGCTGTCTTACGATGAAGACGTCATGGGTCAGCTCGTCTGC
CACGAACTGGTTCCCGGAGGGAAGACCATCCCGGTTACAGATGAGAACAAAATTAGCTACATCCATCTGA
TGGCGCATTTCCGAATGCACACTCAAATTAAAAACCAGACAGCTGCTCTTATCAGTGGCTTCCGTTCCAT
CATCAAGCCCGAGTGGATCCGAATGTTCTCTACCCCTGAGCTTCAGCGCCTCATCTCCGGGGACAACGCT
GAGATAGATCTGGAAGACTTAAAGAAGCACACAGTGTACTACGGTGGCTTTCATGGGAGCCACAGGGTCA
TCATCTGGCTCTGGGATATCCTGGCTTCCGACTTCACGCCTGAAGAAAGAGCCATGTTCCTGAAGTTTGT
GACCAGCTGCTCCCGGCCCCCGCTCCTAGGATTCGCCTACCTCAAGCCTCCGTTCTCCATCCGCTGTGTG
GAAGTATCAGATGACCAGGACACTGGGGATACCCTGGGCAGCGTCCTCAGGGGCTTCTTCACCATCCGCA
AGAGGGAGCCAGGGGGGCGTCTGCCCACATCTTCCACTTGCTTCAACCTGCTTAAGCTGCCCAACTACAG
CAAGAAGAGCGTACTGCGAGAGAAACTACGCTATGCCATCAGCATGAACACGGGCTTTGAGCTCTCCTAA
CACAGCCTGGTCTCGCTACCACAGAGCCCCCAGGAGTGTGGCCAGGGTACCCACAGCCCTCAGACCCTCT
CAATAGCCATGAGACACCCAAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4163877), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26366. Forward Primer - name:026366_F_IRAV59-62_D21_Ube3b, sequence:ACAGCTCCGTGGATGAGC; Reverse Primer - name:026366_R_SP6_IRAV59-62_D21_Ube3b, sequence:CCTTGGGTGTCTCATGGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20304 same embryo
 EMAGE:20307 same embryo
 EMAGE:20306 same embryo
 EMAGE:20308 same embryo
 EMAGE:20305 same embryo
 EurExpress:euxassay_012563 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS