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EMAGE:20312

Mapkapk5 MAP kinase-activated protein kinase 5 ( MGI:1333110)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20312 EMAGE:20312 EMAGE:20312 EMAGE:20312 EMAGE:20312
"Pseudo-wholemount" of euxassay_012494. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012494_01 euxassay_012494_02 euxassay_012494_03 euxassay_012494_04
EMAGE:20312 EMAGE:20312 EMAGE:20312 EMAGE:20312 EMAGE:20312
euxassay_012494_05 euxassay_012494_06 euxassay_012494_07 euxassay_012494_08 euxassay_012494_09
EMAGE:20312 EMAGE:20312 EMAGE:20312 EMAGE:20312 EMAGE:20312
euxassay_012494_10 euxassay_012494_11 euxassay_012494_12 euxassay_012494_13 euxassay_012494_14
EMAGE:20312 EMAGE:20312 EMAGE:20312 EMAGE:20312 EMAGE:20312
euxassay_012494_15 euxassay_012494_16 euxassay_012494_17 euxassay_012494_18 euxassay_012494_19
EMAGE:20312 EMAGE:20312 EMAGE:20312 EMAGE:20312
euxassay_012494_20 euxassay_012494_21 euxassay_012494_22 euxassay_012494_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20312Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20312_wholemount_strong.wlz
20312_wholemount_moderate.wlz
20312_wholemount_weak.wlz
20312_wholemount_possible.wlz
20312_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20312_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31376
Entity Detected:Mapkapk5, MAP kinase-activated protein kinase 5 ( MGI:1333110)
Sequence:sense strand is shown

>T31376
AGAGAAGCAAGCCAGCCAAGTAACAAAGCAGATAGCCCTGGCTCTACAGCACTGTCACTTGCTAAACATT
GCGCACAGAGACCTCAAGCCTGAAAATCTGCTTTTCAAGGATAACTCTCTGGACGCCCCTGTGAAATTAT
GTGACTTTGGGTTTGCTAAAGTTGACCAAGGTGATTTGATGACACCCCAGTTTACCCCTTACTATGTAGC
ACCTCAGGTACTGGAAGCGCAGAGACGGCACCAGAAGGAGAAGTCTGGCATCATACCTACCTCGCCAACA
CCCTACACTTACAACAAGAGCTGTGACTTGTGGTCCCTAGGGGTGATAATTTATGTGATGCTGTGCGGAT
ATCCTCCTTTTTACTCCAAACACCATAGTCGGACTATCCCAAAGGATATGCGGAAAAAGATCATGACAGG
AAGTTTCGAGTTCCCAGAAGAAGAGTGGAGCCAGATCTCAGAGATGGCTAAAGATGTTGTGAGGAAGCTT
CTGAAGGTCAAACCAGAGGAAAGACTCACAATCGAGGGAGTGTTGGACCATCCCTGGCTCAACTCGACAG
AGGCCCTGGATAATGTGCTACCCTCTGCCCAGCTGATGATGGATAAGGCGGTGGTTGCGGGGATCCAGCA
GGCGCACGCCGAGCAGCTGGCAAACATGAGGATCCAGGACCTCAAGGTCAGCCTCAAACCCCTGCACTCT
GTCAACAACCCCATTCTCAGGAAGAGGAAGCTGCTGGGCACCAAGCCAAAGGACGGTATTTATATACACG
ACCATGAGAATGGAACTGAGGACTCAAATGTTGCCTTGGAAAAGCTTCGAGATGTCATTGCCCAGTGTAT
CCTCCCCCAGGCTGGTAAAGGAGAGAATGAAGATGAGAAGCTGAATGAGGTAATGCAGGAGGCCTGGAAG
TACAACCGCGAATGCAAGCTCCTGAGGGATGCTCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5010305), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 24042. Forward Primer - name:024042_F_IRAV55-58_F19_Mapkapk5, sequence:AGAGAAGCAAGCCAGCCA; Reverse Primer - name:024042_R_SP6_IRAV55-58_F19_Mapkapk5, sequence:GCAGAGCATCCCTCAGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20310 same embryo
 EMAGE:20309 same embryo
 EMAGE:20311 same embryo
 EurExpress:euxassay_012494 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS