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EMAGE:20351

Acer3 alkaline ceramidase 3 ( MGI:1913440)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20351 EMAGE:20351 EMAGE:20351 EMAGE:20351 EMAGE:20351
"Pseudo-wholemount" of euxassay_012582. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012582_01 euxassay_012582_02 euxassay_012582_03 euxassay_012582_04
EMAGE:20351 EMAGE:20351 EMAGE:20351 EMAGE:20351 EMAGE:20351
euxassay_012582_05 euxassay_012582_06 euxassay_012582_07 euxassay_012582_08 euxassay_012582_09
EMAGE:20351 EMAGE:20351 EMAGE:20351 EMAGE:20351 EMAGE:20351
euxassay_012582_10 euxassay_012582_11 euxassay_012582_12 euxassay_012582_13 euxassay_012582_14
EMAGE:20351 EMAGE:20351 EMAGE:20351 EMAGE:20351 EMAGE:20351
euxassay_012582_15 euxassay_012582_16 euxassay_012582_17 euxassay_012582_18 euxassay_012582_19
EMAGE:20351 EMAGE:20351 EMAGE:20351 EMAGE:20351 EMAGE:20351
euxassay_012582_20 euxassay_012582_21 euxassay_012582_22 euxassay_012582_23 euxassay_012582_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20351Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20351_wholemount_strong.wlz
20351_wholemount_moderate.wlz
20351_wholemount_weak.wlz
20351_wholemount_possible.wlz
20351_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20351_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vibrissa
moderate moderate
regionalmoderate expression: see section 04 05 06 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31544
Entity Detected:Acer3, alkaline ceramidase 3 ( MGI:1913440)
Sequence:sense strand is shown

>T31544
TTGGTGCAATTCAAGGCATTAGAGACAGACTGGAGAAGCGGTACATTGCTGCTTACTTAGCACTCACAGT
GGTAGGAATGGGATCCTGGTGTTTCCACATGACTCTGAAATATGAAATGCAGCTGTTGGATGAGCTCCCC
ATGATTTACAGCTGCTGCATATTTGTATACTGCATGTTTGAGTGTTTCAAGACAAAGAGCTCAATAAACT
ACCATCTTCTTTTTACCCTATTTCTATACAGTTTAACAGTAACTACGATTTACCTAAAAGTCAAAGAACC
TATATTCCATCAGGTCATGTATGGAATGTTGGTCTTTACATTAGTACTTCGTTCTATTTATATTGTTACA
TGTGTATCTCCAGAGTCTTGTCTGTACTGAGCATGAATGGACCATGTGCTGATGAAGTTCTTGCTCTCTC
CTTCGCGCCCTGCTACCTGGATAACCAAAGACATTCATTTCACCCTACTGAAGAATTTTGCTTTGTACTT
CTTCTGAGAACTGAAGATTTGCCATGGGTTTATCCATGGCTTAGAGGACTAGGTTATACATCCTTAACTG
TCTTTTTATTGGGGTTTTTATTGTGGAATATAGATAACATCTTTTGTGATTCACTGAGGAACTTTCGAAA
GAGAGTGCCCCCCGTCCTAGGTGTTACAACACAGTTTCATGCATGGTGGCATATTCTAACTGGCCTGGGT
TCTTATCTTCACATCCTTTTCAGTTTATATACAAGAACACTTTACCTGAGGTACAGGCCAAAAGTGAAGT
TTCTCTTTGGAATCTGGCCAGCAGTCATGTTTGAACCTCAGAGGAAGCACTGATGAATTATTCTACCAAG
AAAATAAAAAACACCTACTGTATGTAGTTCTGCCAAGATGGTCAAGAACCCCCCAAAGACTTGTACATTT
GAAGACACCATGCCCATCAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5324078), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 29623. Forward Primer - name:029623_F_IRAV67-70_J20_Phca, sequence:TTGGTGCAATTCAAGGCA; Reverse Primer - name:029623_R_SP6_IRAV67-70_J20_Phca, sequence:TGTGATGGGCATGGTGTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20353 same embryo
 EMAGE:20350 same embryo
 EMAGE:20352 same embryo
 EMAGE:20349 same embryo
 EurExpress:euxassay_012582 same experiment
 MGI:4822918 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS