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EMAGE:20375

BC029214 cDNA sequence BC029214 ( MGI:2442831)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20375 EMAGE:20375 EMAGE:20375 EMAGE:20375 EMAGE:20375
"Pseudo-wholemount" of euxassay_012628. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012628_01 euxassay_012628_02 euxassay_012628_03 euxassay_012628_04
EMAGE:20375 EMAGE:20375 EMAGE:20375 EMAGE:20375 EMAGE:20375
euxassay_012628_05 euxassay_012628_06 euxassay_012628_07 euxassay_012628_08 euxassay_012628_09
EMAGE:20375 EMAGE:20375 EMAGE:20375 EMAGE:20375 EMAGE:20375
euxassay_012628_10 euxassay_012628_11 euxassay_012628_12 euxassay_012628_13 euxassay_012628_14
EMAGE:20375 EMAGE:20375 EMAGE:20375 EMAGE:20375 EMAGE:20375
euxassay_012628_15 euxassay_012628_16 euxassay_012628_17 euxassay_012628_18 euxassay_012628_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20375Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20375_wholemount_strong.wlz
20375_wholemount_moderate.wlz
20375_wholemount_weak.wlz
20375_wholemount_possible.wlz
20375_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20375_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31611
Entity Detected:BC029214, cDNA sequence BC029214 ( MGI:2442831)
Sequence:sense strand is shown

>T31611
CTTGAGTACCGCCCATCGCCTCGTCCTTCCGTCCCTCCAGAAACCAGAAGTTCCGCCGCCGAGCGAACAC
CTTAGATTGTGAGTCACCGAGTTTGTACTTCTGCGCAGGCGCCCTCAGCCCACATCTAACTTGGCTCCTG
CGGCGAGACCGCCCTGCTGTCATGGCTCCTCCGTTGTTGTCGCTGCCGCTTTGTATTCTGCCGCCGGGTT
CGGGCTCCCCCCGCCTGGTGTGCTACTGCGAGCGGGATAGTGGTGGAGACGGGGACCGCGACGACTTCAA
CCTCTATGTGACGGACGCCGCCGAGCTCTGGAGCACCTGCTTCTCGCCTGACAGCCTGGCGAGGCTCAAA
GCCCGATTTGGCCTAAGTGGGGCTGAAGATATCCATTCCCGGTTCAGGGCAGCCTGCCAACAGCAGGCAG
TAACTGTAAGCCTGCAAGAAGACAGAGCATTGATAACCCTTTCAGGGGATACCCCTGCCCTGGCCTTTGA
CCTCTCCAAGGTGCCCAGCCCGGAGGCAGCCCCCAGGCTGCAGGCTTTGACTCTCAGCCTGGCAGAGCAT
GTATGCAACCTAGAGAGGCGGCTGGCAGCTGCAGAAGAGACCATCACTAGCCCCAAGAAGAACACCCAGC
CAGCAGGCACTCAGTTCTTACCAGAACTTGATCACCAGAGAGGCAGCTCTGGGCCTGGGGTCAGGAGACG
GTGTCCAGGGGAGTCTCTCATTAACCCTGGCTTCAAAAGTAAGAAACCAGCTGCTGGTGTAGACTTTGAT
GAGACCTGAGGACAGCAAAGCCTGTTACTTGGCCAGGAGCCTGCCTGCAAGGAAAGGGGAGATCTAGGAC
CCCCTAATCAGAGACACCCCTTGCTTCACTTCCCCTGTGGTCCTGCTGTTGGGCAAAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4161391), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 29948. Forward Primer - name:029948_F_IRAV77-80_L13_BC029214, sequence:CTTGAGTACCGCCCATCG; Reverse Primer - name:029948_R_SP6_IRAV77-80_L13_BC029214, sequence:ACCTTTGCCCAACAGCAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20370 same embryo
 EMAGE:20373 same embryo
 EMAGE:20372 same embryo
 EMAGE:20374 same embryo
 EMAGE:20371 same embryo
 EurExpress:euxassay_012628 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS