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EMAGE:20442

Kdm4b lysine (K)-specific demethylase 4B ( MGI:2442355)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20442 EMAGE:20442 EMAGE:20442 EMAGE:20442 EMAGE:20442
"Pseudo-wholemount" of euxassay_009148. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009148_01 euxassay_009148_02 euxassay_009148_03 euxassay_009148_04
EMAGE:20442 EMAGE:20442 EMAGE:20442 EMAGE:20442 EMAGE:20442
euxassay_009148_05 euxassay_009148_06 euxassay_009148_07 euxassay_009148_08 euxassay_009148_09
EMAGE:20442 EMAGE:20442 EMAGE:20442 EMAGE:20442 EMAGE:20442
euxassay_009148_10 euxassay_009148_11 euxassay_009148_12 euxassay_009148_13 euxassay_009148_14
EMAGE:20442 EMAGE:20442 EMAGE:20442 EMAGE:20442 EMAGE:20442
euxassay_009148_15 euxassay_009148_16 euxassay_009148_17 euxassay_009148_18 euxassay_009148_19
EMAGE:20442 EMAGE:20442 EMAGE:20442 EMAGE:20442 EMAGE:20442
euxassay_009148_20 euxassay_009148_21 euxassay_009148_22 euxassay_009148_23 euxassay_009148_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20442Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20442_wholemount_strong.wlz
20442_wholemount_moderate.wlz
20442_wholemount_weak.wlz
20442_wholemount_possible.wlz
20442_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20442_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 24
facial vii ganglion
strong strong
regionalstrong expression: see section 04 05 06 19 20 21
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 07 08 18 19
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 15 17 18 19 20 21 22
vagus x ganglion
strong strong
regionalstrong expression: see section 08 18 19
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 07 08 19
trigeminal v nerve
strong strong
regionalstrong expression: see section 16
spinal cord
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17 18
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 17 moderate expression: see section 11
cervical ganglion
strong strong
regionalstrong expression: see section 09 17
dorsal root ganglion
strong strong
regionalstrong expression: see section 08 09 10 11 12 15 16 17 18 19
neural retina
strong strong
regionalstrong expression: see section 01 02 03 21 22 moderate expression: see section 23
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 13 17 moderate expression: see section 06 07 14 weak expression: see section 15 16
vomeronasal organ
strong strong
regionalstrong expression: see section 11
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2422
Entity Detected:Kdm4b, lysine (K)-specific demethylase 4B ( MGI:2442355)
Sequence:sense strand is shown

>T2422
TGGCCTCGAGNCAGATTCGGACGAGGCACTGGCACACCACAGGAGCCCAGCTTCTCTGGAGACGATGTGA
AGGCTGCCAAGCGCCCACGGGTGGCCTCAGTGCTGGCCACCACCACTGAGGACACAGGGCGTAGCCCAGA
ATACCTGTCCTTCATGGAGAGCCTGCTGCAGGCACAGGGCCGGCCTGGAGCACCCTTCTAGATCCTGCCC
ACTCCTGTCCCACTGTAGGTAACTGGTAACAGGACCAGCAGGATCCAACTTGGCCAGACTCAGGGAGTGG
GGCCAGCACCCCTGGACTACAGTGACTCCATGGGGCCTGGCCTGGCTCTGCTCTTCTCCTAAAGGTGCTA
AGGGGCATCCTAGAGCCTCCTGTGACAGATGCTAGCCAAGGACACTGGGGCACAGCCCCAACCTGTTTTC
TAGATTTGTGGCTTTAAAAACTGACAGCAGGCACATTCTTTTTCCTCTAAACCAGGATGTGAGGAGATAA
AAGTTTCATCTGTGTTTGACACCTGCAGATGTTTGTCTACTGATCAGGTCGA
Notes:The probe template was PCR amplified from IMAGE:1230747 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1230747 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20441 same embryo
 EMAGE:20445 same embryo
 EMAGE:20440 same embryo
 EMAGE:20444 same embryo
 EMAGE:20443 same embryo
 EurExpress:euxassay_009148 same experiment
 MGI:4825744 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS