EMAGE mouse gene expression spatial database

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EMAGE:20465

Cdk16 cyclin-dependent kinase 16 ( MGI:97516)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012715. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012715_01	euxassay_012715_02	euxassay_012715_03	euxassay_012715_04

euxassay_012715_05	euxassay_012715_06	euxassay_012715_07	euxassay_012715_08	euxassay_012715_09

euxassay_012715_10	euxassay_012715_11	euxassay_012715_12	euxassay_012715_13	euxassay_012715_14

euxassay_012715_15	euxassay_012715_16	euxassay_012715_17	euxassay_012715_18	euxassay_012715_19

euxassay_012715_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20465_wholemount_strong.wlz

20465_wholemount_moderate.wlz

20465_wholemount_weak.wlz

20465_wholemount_possible.wlz

20465_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20465_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 14

pons mantle layer

moderate

regional

moderate expression: see section 07 15

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 14 15 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 07 15

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 16

ventral grey horn

moderate

regional

moderate expression: see section 09 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38210
Entity Detected:	Cdk16, cyclin-dependent kinase 16 ( MGI:97516)
Sequence:	sense strand is shown >T38210 CTACAAAAGGAGGCCAACATTCGGTCCACTTCTATGCCTGACTCAGGCAGGCCAGCTTTCCGTGTGGTGG ATACCGAGTTCTAACCCAAGTTTTAAGCCACAGACAGACCAAGGCCCCAGCAGGCAGCGGCTGGAGGGAT GCCACACCCCTTACAGGGCAGCCCCCATCTGCAATCCTCCCTGCTTGTTGCCTGCTTACCTGCCTGAGCC ACACTCCCCTGCCAACTTGTCCCCTGCCACCTGCCCAAACACCAAACCACTGGCCTGGCCTGTCAACCCA ACCATTGGCCTGTCTGCTGGGTGCTAACAAAGCTCTCACCACTACTTTGCTTGATGTGTCTGTCTCTGTC TTGGTAGTTGCTGGTGAACAGAATGGCCATGCCAGGTTTCCACACTAAGGCTAGGCCTTCCCCTCTAAAT CACACTCTCTCCCAGGACCACTACCCCATGGCCAGCCAGGGATTTGGAGCTAGCCCAGGCCAGGCTCTTA ATTGACTTTGACTAGAAGGTAGTGAGTGATGCCTTGGGTCTGAGCATCCTCTGCCTGCTTGCCACCTGTC CCACTTGCCTCTGTTGTATGGGCCTTCTTTTTTGTTAGTTTCTTTTATTGTTTTTTTATTATTTTAAATG AGGTTCTCACTTTTTAATGCAATATCTCTGTATACAGACTGGTTGGGCGCTAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 146446. Forward Primer - name:146446_F_cDNA_Pctk1, sequence:CTACAAAAGGAGGCCAACATTC; Reverse Primer - name:146446_N_SP6_cDNA_Pctk1, sequence:GTAGCGCCCAACCAGTCTGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20469 same embryo
	EMAGE:20464 same embryo
	EMAGE:20468 same embryo
	EMAGE:20467 same embryo
	EMAGE:20466 same embryo
	EurExpress:euxassay_012715 same experiment
	MGI:4823777 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source