EMAGE mouse gene expression spatial database

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EMAGE:20523

Polb polymerase (DNA directed), beta ( MGI:97740)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012784. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012784_01	euxassay_012784_02	euxassay_012784_03	euxassay_012784_04

euxassay_012784_05	euxassay_012784_06	euxassay_012784_07	euxassay_012784_08	euxassay_012784_09

euxassay_012784_10	euxassay_012784_11	euxassay_012784_12	euxassay_012784_13	euxassay_012784_14

euxassay_012784_15	euxassay_012784_16	euxassay_012784_17	euxassay_012784_18	euxassay_012784_19

euxassay_012784_20	euxassay_012784_21	euxassay_012784_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20523_wholemount_strong.wlz

20523_wholemount_moderate.wlz

20523_wholemount_weak.wlz

20523_wholemount_possible.wlz

20523_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20523_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 10 11 13

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 17 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 16 17

trigeminal v nerve

moderate

regional

moderate expression: see section 07 15

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

cervico-thoracic ganglion

weak

regional

weak expression: see section 09 15

cervical ganglion

weak

regional

weak expression: see section 07 15 16

thoracic ganglion

weak

regional

weak expression: see section 12

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 13 14 15 16

neural retina

moderate

regional

moderate expression: see section 01 21 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 07 08 09 10 12 13 14 15 16

vomeronasal organ

moderate

regional

moderate expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31951
Entity Detected:	Polb, polymerase (DNA directed), beta ( MGI:97740)
Sequence:	sense strand is shown >T31951 TTCCACCGGTAAGACCCAGGTGTGGGTCCCCGGCGCCATGAGCAAACGCAAGGCGCCGCAGGAGACCCTC AACGGCGGCATCACGGACATGCTCGTGGAACTCGCAAACTTTGAGAAGAACGTGAGCCAGGCGATCCACA AGTACAATGCGTACAGAAAAGCGGCATCTGTGATAGCCAAGTACCCACACAAAATCAAGAGCGGAGCGGA AGCTAAGAAACTGCCAGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAATTTTTAGCAACTGGAAAA TTGCGTAAACTGGAAAAGATTCGTCAAGATGATACAAGTTCATCCATCAATTTCCTGACTCGAGTTACTG GCATTGGACCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAGACATTAGAAGATCTCAGGAAAAA TGAAGATAAACTGAACCATCATCAACGAATTGGGCTGAAATATTTCGAGGACTTTGAAAAGAGAATTCCT CGTGAGGAAATGCTGCAGATGCAGGATATTGTACTTAATGAAATTAAAAAAGTGGACTCTGAGTACATTG CTACAGTCTGTGGCAGTTTCAGAAGAGGCGCAGAGTCGAGTGGAGACATGGATGTCCTGCTGACCCACCC AAACTTCACGTCAGAGTCCAGCAAACAGCCAAAGTTGTTACATCGTGTTGTGGAACAGTTACAAAAAGTC CATTTCATTACAGATACTCTGTCAAAGGGTGAAACAAAGTTCATGGGTGTTTGCCAGCTTCCCAGCGAGA AGGATGGAAAGGAATATCCACACAGGAGAATCGATATCAGGTTGATCCCCAAAGATCAGTACTACTGTGG TGTTCTCTACTTCACTGGGAGTGACATCTTTAATAAGAACATGAGAGCGCACGCCCTGGAAAAGGGCTTC ACAATCAATGAGTACACCATCCGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6774075), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 61214. Forward Primer - name:061214_F_IRAW6_h10_Polb, sequence:TTCCACCGGTAAGACCCA; Reverse Primer - name:061214_R_SP6_IRAW6_h10_Polb, sequence:GGGCGGATGGTGTACTCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20521 same embryo
	EMAGE:20522 same embryo
	EMAGE:20525 same embryo
	EMAGE:20524 same embryo
	EMAGE:20520 same embryo
	EurExpress:euxassay_012784 same experiment
	MGI:4827313 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source