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EMAGE:20608

Olfr982 olfactory receptor 982 ( MGI:3030816)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20608 EMAGE:20608 EMAGE:20608 EMAGE:20608 EMAGE:20608
"Pseudo-wholemount" of euxassay_013894. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013894_01 euxassay_013894_02 euxassay_013894_03 euxassay_013894_04
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euxassay_013894_05 euxassay_013894_06 euxassay_013894_07 euxassay_013894_08 euxassay_013894_09
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euxassay_013894_10 euxassay_013894_11 euxassay_013894_12 euxassay_013894_13 euxassay_013894_14
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euxassay_013894_15 euxassay_013894_16 euxassay_013894_17 euxassay_013894_18 euxassay_013894_19
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euxassay_013894_20 euxassay_013894_21 euxassay_013894_22 euxassay_013894_23 euxassay_013894_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20608Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20608_wholemount_strong.wlz
20608_wholemount_moderate.wlz
20608_wholemount_weak.wlz
20608_wholemount_possible.wlz
20608_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20608_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
moderate moderate
single cellmoderate expression: see section 11 12 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39141
Entity Detected:Olfr982, olfactory receptor 982 ( MGI:3030816)
Sequence:sense strand is shown

>T39141
CTTTATTGCTTCCACTTCCTGGCCAGCACTGAGTGTTTTTTATACACAGTCATGGCCTATGACCGTTACC
TGGCTATCTGTCAGCCCCTGCACTACCCAGTGGTTATGAACAAGCGGGTGTGCGCAGGGTTGGCTGGGAG
CACATGGGCCATAGGTGCTATGCATTCTGCAATTCACACTTCCCTAACCTTCCGCCTGCTGTACTGTGGG
CCTCAGCATATTGCCTACTTCTTCTGTGACATCCCTCCAGTACTGAAGCTGGCCTGTGCTGACACCACCA
TCAACGAGCTTGTCATGCTTGCAAACATTGGTGTGGTGGCTGCGGGCTGCCTCATTCTCATCATCATCTC
CTACGCCTTCATTGTGGCTGCGGTGTTGCGCATTCGCACAGCGGAGGGGCGTCAGCGAGCCTTCTCCACT
TGCACAGCACATCTCACCGTGGTGCTCCTGTATTACATGCCCCCGGTCTGTATCTACCTGCAGCCAAGCT
CCACTGGCGCAGGGGCGGGAGCTCCAGCAGTCTTCTACACAATAGTCACTCCAATGCTCAATCCTTTCAT
CTACACACTAAGAAACAAGGAAGTCAAGCGGGCTCTGAGAAGGTTA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 152492. Forward Primer - name:152492_F_cDNA_Olfr982, sequence:CTTTATTGCTTCCACTTCCTGG; Reverse Primer - name:152492_N_SP6_cDNA_Olfr982, sequence:TAACCTTCTCAGAGCCCGCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20610 same embryo
 EMAGE:20613 same embryo
 EMAGE:20609 same embryo
 EMAGE:20612 same embryo
 EMAGE:20611 same embryo
 EurExpress:euxassay_013894 same experiment
 MGI:4826965 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS