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EMAGE:20643

Olfr1112 olfactory receptor 1112 ( MGI:3030946)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20643 EMAGE:20643 EMAGE:20643 EMAGE:20643 EMAGE:20643
"Pseudo-wholemount" of euxassay_013926. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013926_01 euxassay_013926_02 euxassay_013926_03 euxassay_013926_04
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euxassay_013926_05 euxassay_013926_06 euxassay_013926_07 euxassay_013926_08 euxassay_013926_09
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euxassay_013926_10 euxassay_013926_11 euxassay_013926_12 euxassay_013926_13 euxassay_013926_14
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euxassay_013926_15 euxassay_013926_16 euxassay_013926_17 euxassay_013926_18 euxassay_013926_19
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euxassay_013926_20 euxassay_013926_21 euxassay_013926_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20643Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20643_wholemount_strong.wlz
20643_wholemount_moderate.wlz
20643_wholemount_weak.wlz
20643_wholemount_possible.wlz
20643_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20643_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
moderate moderate
single cellmoderate expression: see section 10 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39166
Entity Detected:Olfr1112, olfactory receptor 1112 ( MGI:3030946)
Sequence:sense strand is shown

>T39166
TGTCAGTCACTCTGCCAAGGTTATTAACAGATCTCTACAGACAAGACAGAATCATTTCTTTCATGGCTTG
TGCTACACAAATGTATTTTTTTCTTATCTTTGGGGCCACTGAGTGCTTTATTCTGACGGCTATGGCTTAT
GACAGATATGTTGCTATTTGTAACCCATTGCTCTATCCTCTCATTATGAACAATAGTCTCTGTATACAAC
TGGCAGCTGGCTGTTGGATAAGTGGAGTTCCTGTTCATATAGGGTTCACATACTGGATCTTCTCTCTACC
ATTTTGTGGATCAAATCAGCTGAATCACTTTTTCTGTGACATACCTCCAGTCCTGACCCTAGCTTGTGGG
GACACTTTTATGATAGAGATGCTAATTTATGTGATTGCCCTTTTAGTTGTCACCATTCCTTTTATGCTGA
TCCTTGCATCTTATGTGAAAATAATCTCAAGCATTCTGAAGCTTCCATCTGCAACTGGGAGAGCCAAGGC
CTTCTCTACATGTTCTTCCCACCTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 279174. Forward Primer - name:279174_F_cDNA_Olfr1112, sequence:TGTCAGTCACTCTGCCAAGG; Reverse Primer - name:279174_N_SP6_cDNA_Olfr1112, sequence:AAGGTGGGAAGAACATGTAGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20644 same embryo
 EMAGE:20645 same embryo
 EurExpress:euxassay_013926 same experiment
 MGI:4826864 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS