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EMAGE:20677

Olfr15 olfactory receptor 15 ( MGI:106182)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20677 EMAGE:20677 EMAGE:20677 EMAGE:20677 EMAGE:20677
"Pseudo-wholemount" of euxassay_013941. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013941_01 euxassay_013941_02 euxassay_013941_03 euxassay_013941_04
EMAGE:20677 EMAGE:20677 EMAGE:20677 EMAGE:20677 EMAGE:20677
euxassay_013941_05 euxassay_013941_06 euxassay_013941_07 euxassay_013941_08 euxassay_013941_09
EMAGE:20677 EMAGE:20677 EMAGE:20677 EMAGE:20677 EMAGE:20677
euxassay_013941_10 euxassay_013941_11 euxassay_013941_12 euxassay_013941_13 euxassay_013941_14
EMAGE:20677 EMAGE:20677 EMAGE:20677 EMAGE:20677 EMAGE:20677
euxassay_013941_15 euxassay_013941_16 euxassay_013941_17 euxassay_013941_18 euxassay_013941_19
EMAGE:20677 EMAGE:20677 EMAGE:20677 EMAGE:20677
euxassay_013941_20 euxassay_013941_21 euxassay_013941_22 euxassay_013941_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20677Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20677_wholemount_strong.wlz
20677_wholemount_moderate.wlz
20677_wholemount_weak.wlz
20677_wholemount_possible.wlz
20677_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20677_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
strong strong
single cellstrong expression: see section 09 10 11 12 13 14 15 16 17 18 19
vomeronasal organ
strong strong
single cellstrong expression: see section 13 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39204
Entity Detected:Olfr15, olfactory receptor 15 ( MGI:106182)
Sequence:sense strand is shown

>T39204
AAAATTTATGGGGGCCAGACAAGACAATCAGCTATGGTGGGTGTGTAACTCAACTCTATGTTTTCCTTTG
GCTGGGGGCTACTGAGTGCATACTGCTCGTGGTGATGGCATTTGATCGGTATGTGGCAGTTTGTCGGCCC
CTGCACTACATGACCGTCATGAATCCTCGCCTCTGCTGGGGGCTGGCTGCTATTAGCTGGTTGGGTGGCT
TAGGCAACTCCGTGATTCAGTCAACATTCACTCTCCAGCTCCCATTTTGCGGACACCGAAAAGTGGACAA
CTTCCTGTGTGAGGTACCCGCCATGATTAAATTGGCCTGTGGAGACACAAGTCTCAATGAGGCGGTGCTC
AATGGTGTTTGTACCTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 278619. Forward Primer - name:278619_F_cDNA_Olfr15, sequence:AAAATTTATGGGGGCCAGAC; Reverse Primer - name:278619_N_SP6_cDNA_Olfr15, sequence:GAAGGTACAAACACCATTGAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20676 same embryo
 EMAGE:20679 same embryo
 EMAGE:20678 same embryo
 EMAGE:20675 same embryo
 EMAGE:20680 same embryo
 EurExpress:euxassay_013941 same experiment
 MGI:4826902 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS