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EMAGE:20702

Lrrc51 leucine rich repeat containing 51 ( MGI:1916608)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20702 EMAGE:20702 EMAGE:20702 EMAGE:20702 EMAGE:20702
"Pseudo-wholemount" of euxassay_014006. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014006_01 euxassay_014006_02 euxassay_014006_03 euxassay_014006_04
EMAGE:20702 EMAGE:20702 EMAGE:20702 EMAGE:20702 EMAGE:20702
euxassay_014006_05 euxassay_014006_06 euxassay_014006_07 euxassay_014006_08 euxassay_014006_09
EMAGE:20702 EMAGE:20702 EMAGE:20702 EMAGE:20702 EMAGE:20702
euxassay_014006_10 euxassay_014006_11 euxassay_014006_12 euxassay_014006_13 euxassay_014006_14
EMAGE:20702 EMAGE:20702 EMAGE:20702 EMAGE:20702 EMAGE:20702
euxassay_014006_15 euxassay_014006_16 euxassay_014006_17 euxassay_014006_18 euxassay_014006_19
EMAGE:20702 EMAGE:20702 EMAGE:20702 EMAGE:20702
euxassay_014006_20 euxassay_014006_21 euxassay_014006_22 euxassay_014006_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20702Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20702_wholemount_strong.wlz
20702_wholemount_moderate.wlz
20702_wholemount_weak.wlz
20702_wholemount_possible.wlz
20702_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20702_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lateral ventricle choroid plexus
weak weak
regionalweak expression: see section 05 06 07 08 13 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31601
Entity Detected:Lrrc51, leucine rich repeat containing 51 ( MGI:1916608)
Sequence:sense strand is shown

>T31601
ACACTTCCGTGCAGGAGCCTCCTCTTGACTACTCCTTCAAAAGCGTCCAAATGGTCCAAGATCTGGTAAC
CGAGGAGCCAAGGACAGGTCTACGACCGGTGAGGCACTCAAAGTCAGGAAAGTCACTGACCCAGTCCCTG
TGGCTCAACAACAATGTCCTCAATGATCTGAAAGACTTCAACCAGGTGGTTTCACAGCTTCTACAGCATC
CAGAGAATCTGGCCTGGATTGACCTCTCCTTCAATGACCTGACTACCATTGACCCTGTCCTAACAACATT
CTTCAACCTAAGTGTCCTCTACCTTCACGGCAACGGCATCCATCGCCTAGGGGAGGTGAATAAGCTAGCT
GTCCTCCCTCGTCTCCGGAGCCTGACCCTCCATGGGAACCCCATAGAGGAAGAAAAGGGGTACAGGTAAG
TGCCTGGATCTAAGGTCCAGATGAGTTTCCCTAAGGAACAAGGGGAGAGGAGACAAGAAATTCAAGACCA
TTCTTCTGCCACACGTGGACCCAGGACGTACCAGACCTCTAAGCATGCCCCTTGCTCTCAGAATGAACCC
AGTCCTCCCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4225298), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 63439. Forward Primer - name:063439_F_IRAV77-80_F12_1700008D07Rik, sequence:ACACTTCCGTGCAGGAGC; Reverse Primer - name:063439_R_SP6_IRAV77-80_F12_1700008D07Rik, sequence:TTGGGAGGACTGGGTTCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20705 same embryo
 EMAGE:20704 same embryo
 EMAGE:20706 same embryo
 EMAGE:20703 same embryo
 EurExpress:euxassay_014006 same experiment
 MGI:4825998 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS