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EMAGE:20726

Mdfic MyoD family inhibitor domain containing ( MGI:104611)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20726 EMAGE:20726 EMAGE:20726 EMAGE:20726 EMAGE:20726
"Pseudo-wholemount" of euxassay_013974. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013974_01 euxassay_013974_02 euxassay_013974_03 euxassay_013974_04
EMAGE:20726 EMAGE:20726 EMAGE:20726 EMAGE:20726 EMAGE:20726
euxassay_013974_05 euxassay_013974_06 euxassay_013974_07 euxassay_013974_08 euxassay_013974_09
EMAGE:20726 EMAGE:20726 EMAGE:20726 EMAGE:20726 EMAGE:20726
euxassay_013974_10 euxassay_013974_11 euxassay_013974_12 euxassay_013974_13 euxassay_013974_14
EMAGE:20726 EMAGE:20726 EMAGE:20726 EMAGE:20726 EMAGE:20726
euxassay_013974_15 euxassay_013974_16 euxassay_013974_17 euxassay_013974_18 euxassay_013974_19
EMAGE:20726 EMAGE:20726 EMAGE:20726
euxassay_013974_20 euxassay_013974_21 euxassay_013974_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20726Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20726_wholemount_strong.wlz
20726_wholemount_moderate.wlz
20726_wholemount_weak.wlz
20726_wholemount_possible.wlz
20726_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20726_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18
choroid invagination
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 13 14 15 16
metencephalon part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39405
Entity Detected:Mdfic, MyoD family inhibitor domain containing ( MGI:104611)
Sequence:sense strand is shown

>T39405
GCAAGAGGCCATAGGAACATACAGAAGTGAATAACAGGTTACAGAAGAAGGCCTGGAGGAAGATATGGCA
CTGTGGATGCTCTAGGACCAGCTACTTGAATTTAAGGAAATGTTGAAGGTACACAGCCTAGACTGTCAGT
GCTTTGGACAAGTGAAAAGGATTTGCCTTGTATATCGCAGCAACACAGCTTTCTTAGAACTGTAAAATCT
CAACTAATGCAAATACGCACTTTCCTACATGTAATTTAAGTGTTATAAATTTTAATGAAAACATAATACC
TTGTTAACATAACTATATTTTACCAAAAAATGTAGAACACTTTTTAACAACGTTCATTTAAATGAGCTGG
TTTTTTTTCCTTTTTTTTAAGATTAAAAAAATCCCAGTATTCCTTGGGGTTTTTCCAAAGCTGCCTTATA
TTTGGAAAGACTCGGTTCTATTTATTTTCATTGGCTTGTAGTCCTAGCCATGTTGTCTTTCATAAGGAGG
TGAGTTTTGGATCCACTACAGGAGGACTTCCGACGTCCAACACCATCCACCAGGTATCAGGATTCCAACT
GTGTGTGAAATTATGCCAAGTGACAGGTTATAAAGATACTAAAGTACGAAGAAGAGGGGCTGCAATGAGA
CTAATTCTACAGTGATGGAGTCAGCTCATGTGGAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 93534. Forward Primer - name:093534_F_cDNA_Kdt1, sequence:GCAAGAGGCCATAGGAACATAC; Reverse Primer - name:093534_N_SP6_cDNA_Kdt1, sequence:CTCCACATGAGCTGACTCCAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20728 same embryo
 EMAGE:20727 same embryo
 EMAGE:20729 same embryo
 EMAGE:20730 same embryo
 EurExpress:euxassay_013974 same experiment
 MGI:4826140 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS