EMAGE mouse gene expression spatial database

EMAGE:20739

Olfr214 olfactory receptor 214 ( MGI:3030048)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013945. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013945_01	euxassay_013945_02	euxassay_013945_03	euxassay_013945_04

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

single cell

strong expression: see section 17 moderate expression: see section 13 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39211
Entity Detected:	Olfr214, olfactory receptor 214 ( MGI:3030048)
Sequence:	sense strand is shown >T39211 GTTTCTCCTTTTGCGAGTGCTGTTTCATCTCCACCGTTATCCCGAAGCTCCTGGCGATCTTTCTTTTTGG AGACAGAACAATTCATTTCACTCCCTGTATCATCCAAGCGTTTTCTTTTTTGTTTCTTGGGTCCACAATT TTCTTCCACATGGCTGTGATGTCCTTGGATCGCTACCTAGCCATTTGCAAGCCTCTGCGCTACCCAGCCA TCATGAACCCCAGGGTCTGTTTCCTTCTGGTTTTCTTCAGCTATGTGCTTTCCTTCATCCTAGTAACCGG TGTGATTCTGAGGCTTTCCAGGCTGTCTTTCTGTGGCTCCAATGTCATTCCCCACTTCTTCTGTGACCTT GGCTCCTTAATTCATCTCTCCTGTTCAGACACCAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 279134. Forward Primer - name:279134_F_cDNA_Olfr214, sequence:GTTTCTCCTTTTGCGAGTGC; Reverse Primer - name:279134_N_SP6_cDNA_Olfr214, sequence:TTGGTGTCTGAACAGGAGAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20741 same embryo
	EMAGE:20740 same embryo
	EMAGE:20742 same embryo
	EurExpress:euxassay_013945 same experiment
	MGI:4826907 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source