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EMAGE:21038

Nmur2 neuromedin U receptor 2 ( MGI:2441765)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21038 EMAGE:21038 EMAGE:21038 EMAGE:21038 EMAGE:21038
"Pseudo-wholemount" of euxassay_008390. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008390_01 euxassay_008390_02 euxassay_008390_03 euxassay_008390_04
EMAGE:21038 EMAGE:21038 EMAGE:21038 EMAGE:21038 EMAGE:21038
euxassay_008390_05 euxassay_008390_06 euxassay_008390_07 euxassay_008390_08 euxassay_008390_09
EMAGE:21038 EMAGE:21038 EMAGE:21038 EMAGE:21038 EMAGE:21038
euxassay_008390_10 euxassay_008390_11 euxassay_008390_12 euxassay_008390_13 euxassay_008390_14
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euxassay_008390_15 euxassay_008390_16 euxassay_008390_17 euxassay_008390_18 euxassay_008390_19
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euxassay_008390_20 euxassay_008390_21 euxassay_008390_22 euxassay_008390_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21038Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21038_wholemount_strong.wlz
21038_wholemount_moderate.wlz
21038_wholemount_weak.wlz
21038_wholemount_possible.wlz
21038_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21038_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35269
Entity Detected:Nmur2, neuromedin U receptor 2 ( MGI:2441765)
Sequence:sense strand is shown

>T35269
TTGAAACTCAGACTTCGCTCTGGGTCCCAGCGCAGACATTACACTCCATCTTTCTGCATTGTAATTTTTT
TTTATAACTGTGATTGTAATGTGCCATGGGAGTCTCACTTAAAGATGCTCTCATGATGGGTGGGTTTAAC
CTAGTAAAGGTGTTGTAGAGTAGCTAAGCAGCACAATACAGTTATCCTAACCTCCATACCCAGGATGCAG
GAGAGACCACTGGGTAGGAACTGCCCGCAAAAGCCTAACCCTCCAAAAGTTAGGACTTTTGGGAGTTTGT
TGTCAAATAAGCAATGGCTTAAAATCAGTCCTAGCAGAAATATTTATGCTACAGAAATTAACAAGCTCTC
TAAATCAGGGCTAAATAATTTTTATCCCCTGGAGAGTTGGCCCATGAGCAGGTTGAGAGCTATTAATGAG
CTGGCGCTGTGCCTACTCGTTGCGATGTGTGGGACTTGTTTTTAGAAGAAATGCTGCTTATGCTGTAATT
ATGAACTACTTAGAGTCGTGGCTAACTCGGAAAAATATGCTATGGACTAGATTTAAAAACAAAACAAAAC
AAAAATGTTCCTACACAAAAATAAGCGAAATACTGCTAATTAAGCCAATGGAAGCAGCATCAGCATTTAT
AATTAACCTGGAGTGGAAAGATTATTTTTGGATGCCATCCATTAACATTAATGCAGCAAACAGCAATGGA
CTGGGTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 71342. Forward Primer - name:071342_F_cDNA_Nmur2, sequence:TTGAAACTCAGACTTCGCTCTG; Reverse Primer - name:071342_N_SP6_cDNA_Nmur2, sequence:ACACCCAGTCCATTGCTGTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_008390 same experiment
 EMAGE:21037 same embryo
 EMAGE:21039 same embryo
 EMAGE:21040 same embryo
 EMAGE:21041 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS