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EMAGE:21050

Cep76 centrosomal protein 76 ( MGI:1923401)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21050 EMAGE:21050 EMAGE:21050 EMAGE:21050 EMAGE:21050
"Pseudo-wholemount" of euxassay_008558. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008558_01 euxassay_008558_02 euxassay_008558_03 euxassay_008558_04
EMAGE:21050 EMAGE:21050 EMAGE:21050 EMAGE:21050 EMAGE:21050
euxassay_008558_05 euxassay_008558_06 euxassay_008558_07 euxassay_008558_08 euxassay_008558_09
EMAGE:21050 EMAGE:21050 EMAGE:21050 EMAGE:21050 EMAGE:21050
euxassay_008558_10 euxassay_008558_11 euxassay_008558_12 euxassay_008558_13 euxassay_008558_14
EMAGE:21050 EMAGE:21050 EMAGE:21050 EMAGE:21050 EMAGE:21050
euxassay_008558_15 euxassay_008558_16 euxassay_008558_17 euxassay_008558_18 euxassay_008558_19
EMAGE:21050 EMAGE:21050 EMAGE:21050 EMAGE:21050 EMAGE:21050
euxassay_008558_20 euxassay_008558_21 euxassay_008558_22 euxassay_008558_23 euxassay_008558_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21050Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21050_wholemount_strong.wlz
21050_wholemount_moderate.wlz
21050_wholemount_weak.wlz
21050_wholemount_possible.wlz
21050_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21050_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35735
Entity Detected:Cep76, centrosomal protein 76 ( MGI:1923401)
Sequence:sense strand is shown

>T35735
AGCCCTCAGATGCAGTAGAGACCTGCATATTTGATTTGAATGATGAGTCCAAGTGGAAACCCATGAGCGA
AGAAGCAATTAAGTCTGTGTGTGCTCCAGGTGCTACTACCTCCCTCCCTCCCTTCCCACCTCTGTGTGCG
TCCACGATTGACGCCTCGGTCACCAGCAATGAAATCGAAATGCAGCTGAGGCTTCTGGTGTCGGAGCACA
GAAAGGACCTTGGCCTCACCACTGTCTGGGAAGACCAGCTCTCCTATCTTTTGTCACCAGCTCTGGCCTC
GTATGAGTTTGAGCGAACAACAAGCATATCTGCAGGCAATGAAGAATTTCAGGATGCCATCAGGAGGGCT
GTACCTGACGGGCACACATTTAAAGGCTTCCCCATACATTTTGTGTATAGAAATGCAAGGCGTGCATTTG
CCACTTGTCTTCGGTCTCCTTTTTGTGAAGAAATAATCTGTTGTCGTGGAGACCAGGTGCGACTGGCAGT
TCGCGTTCGAGTGTTTACCTACCCTGAGTCTGCATGTGCTGTTTGGATCATGTTTGCTTGTAAATATCGC
TCAGTATTATAGGACTAACATTTCCTAAGAATTATTCCTGTGTTTTATTAGAATTGTATATGAGCAGCTG
TGTGTAGGTTTCTTAATTTAAAATCAGCCTTTTGGATGTCATTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85901. Forward Primer - name:085901_F_cDNA_6230425F05Rik, sequence:AGCCCTCAGATGCAGTAGAGAC; Reverse Primer - name:085901_N_SP6_cDNA_6230425F05Rik, sequence:ACAATGACATCCAAAAGGCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_008558 same experiment
 EMAGE:21048 same embryo
 EMAGE:21049 same embryo
 EMAGE:21051 same embryo
 EMAGE:21052 same embryo
 EMAGE:21053 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS