EMAGE mouse gene expression spatial database

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EMAGE:21063

Ntn3 netrin 3 ( MGI:1341188)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008357_01	euxassay_008357_02	euxassay_008357_03	euxassay_008357_04	euxassay_008357_05

euxassay_008357_06	euxassay_008357_07	euxassay_008357_08	euxassay_008357_09	euxassay_008357_10

euxassay_008357_11	euxassay_008357_12	euxassay_008357_13	euxassay_008357_14	euxassay_008357_15

euxassay_008357_16	euxassay_008357_17	euxassay_008357_18	euxassay_008357_19	euxassay_008357_20

euxassay_008357_21	euxassay_008357_22	euxassay_008357_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21063_wholemount_strong.wlz

21063_wholemount_moderate.wlz

21063_wholemount_weak.wlz

21063_wholemount_possible.wlz

21063_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21063_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9200
Entity Detected:	Ntn3, netrin 3 ( MGI:1341188)
Sequence:	sense strand is shown >T9200 CTCGCTGCAGCCAGGAGAAGCCGGCAGCCCGGGCGCCCCAGGCCCCGCCCGCCCAAGGCCCTTCCCGGGA GGCCGGGAGACCTGCTCCTCCTGGCCCTCCGTGGGTGAGTGCTGGCGGCCGGCGGCGGGTGGGGCCTCCG CGGGCGGAGGCACCGGGAGCGGGGGCGACGCCTGTCAACGCTTCAGGCCTAGCAGGAGGACTCGCCAACA TCCCTGCTCCTGTGCTCGGCCCCGGGCGTGCCGGTGGCTGCTCCCACTTCTGGGCCTGTGCTGGGGGCCG CCCTGGTGCTCTGCTATCTTGGCACTGCAGACCCGGCACGCAGAGTAGCGGACTAGGCTCTCTCTTCGAG GGCAGACTCGTTAGGCGGAAGCTGGCTGGTTGACTTACAGCGGCCACGGCCATGCCCACCTGGCTCTGGG GGCTGCTGCTGACCGCGGGCACGCTCTCCGCTGCACTGAGCCCAGGGCTGCCGGCCTCTGCCGACCCCTG CTATGATGAGGCGAGGGAGCCTCGCTCTTGTATTCCTGGCCTTGTGAACGCTGCTCTGGGCCGAGAGGTG CTGGCGTCCAGCACGTGCGGGAGGTCGGCCAATCGCGTCTGCGATTCCTCGGACCCGCAGCGGGCACACT CTGCAGACCTCCTGACCTCTGCTCCGGGCACTGCAAGTCCTCTCTGTTGGCGCTCCGATTTGCTGCAACA GGCACCTTTCAACGTAACCCTCACAGTGCCCCTGGGGAAGGCTTTTGAGCTGGTCTTCGTGAGCCTGCGC TTCTGCTCAGCTCCTCCAACCTCCGTGGCCCTGCTTAAGTCGCAGGACCATGGCCGCAGCTGGGTCCCCT TGGGCTTCTTCTCTTCCAGCTGTACCCTGGACTATGGCCGTCTGCCTGCTCCTGCTGATGGCCCTTCTGG TCCAGGGCCAGAAGCCCTCTGCTTTCCAGCCCCCCAGGCTCAGCCTGATGGTGGAGGCCTTCTGGCCTTC AGTGTGCAGGATGGCAGCCCACAGGGCCTGGATCTGGACAACAGCCCCGTGCTCCAAGACTGGGTGACTG CCACAGATATTCGCATAGTACTCACAAGGCCTGCCATTCAGGGAGACACCAGGGACGGTGGGGTGACAGT CCCCTACTCCTACTCAGCCACTGAGCTTCAGGTGGGAGGTCGATGCAAGTGCAATGGGCATGCCTCACGG TGTCTGTTGGACACCCATGGCCACCTGGTCTGCGACTGCCAGCATGGTACAGAGGGCCCTGATTGCAGCC GCTGCAAGCCCTTCTACTGCGACAGGCCATGGCAGCGGGCTACAGGGCAGGAAGCCCACGCTTGCCTTGC TTGCTCCTGCAACGGCCATGCGCGAAGATGCCGCTTCAACATGGAGCTCTACCGACTGTCCGGCCGCCGC AGTGGGGGCGTGTGCCTCAACTGCCGGCACAATACAGCTGGTCGTCACTGCCACTACTGCCGGGAGGGCT TCTATCGTGATCCAGGCCGTGTCCTGAGTGACCGTCGTGCTTGCAGAGCTTGTGACTGCCACCCAGTTGG TGCTGCTGGCAAAACCTGTAACCAGACCACAGGCCAGTGTCCCTGTAAGGATGGTGTTACTGGCCTCACC TGTAACCGCTGTGCCCCAGGTTTCCAGCAGAGCCGTTCTCCTGTGGCACCTTGCGTTAAGACTCCTGTCC CTGGACCCACCGAAGAAAGCAGTCCTGTGGAGCCACAGGACTGTGAGTCACATTGCAGACCTGCGCGTGG CAGTTACCGAATCAGCCTGAAGAAGTTCTGCCGGAAGGACTATGCGGTGCAGGTGGCAGTGGGTGCACGC GGTGAGGCCCGCGGCTCGTGGACACGCTTTCCGGTAGCGGTGCTTGCTGTGTTCCGCAGCGGCGAGGAAC GCGCTCGACGCGGGAGCAGCGCGCTGTGGGTACCAACTCTAGACGCGGCCTGCGGTTGCCCGCGCCTCCT GCCCGGCCGGCGTTACTTGCTGCTGGGAGGTGGGCCGGGGGCTGCAGCTGGGAGCACAGCGGGCCGGGGA CAGGGGCTCAGTGCTGCCCGTGGAAGCCTCGTGCTGCCTTGGAGAGACGCCTGGACCCGGCGCCTGCGGA GGCTGCAGAGGAGAGAGCGGCGGGGGCGCTGCGGGACCGCCTGAATCTGCAAGCTGGGCGTGGACTGGGC GGACTCAGCTCTCTTATCACTGGGCGCGGCGCGTTCATCAGAGCACTAGGCTGGAAGTGTCACGTGCATC GCCATCTAATTTCCCCCTACCCCCATCCCCGCTTGAAACCTATTTGGCGATACCCTACCCCCAACTTAGA GGAGTATGATGGCCCCTAAGAGCTATCTGGAGGCTCCTAGGGCAGCTCAGAGGACCCTGATCTTATCCCT CTTGGCTGATACCCGACGTCTCTTAAAGGTGTGAACCCGCCTATTCCCAGGGGATGCTAGGATGATCCCT CTGGGGTTTGAGCACATCCCACGGGCCAATGTGATTCATCAACTCTCTGATGCTGTGATACACTAGAGTT CTCCAACCTCTCCAAAACCACTGTTGCCACCATAATTGCCTGTACACCGCTGCAGGGGCTGTGGCTCAAT TGCACAGCCAAGACAGCGACGTCTCTGCCCTCCAGCCGGGAATGTACAAAGAGTATTGTAGGCTCCCCTG TCCAGTGTCATGGATCTTGTCCCCATATCCATTCTGGCCTGCCTCTGCCGGTCACGTGACCGTGTCCCCA TCCGCAGCTGCATCACTGCCTGTGTCCCATATCTTTCTTGTTCCGTTGTACCCTTTTCTGGGGTCCATCC AGCTGTCGCTTTCTACCACGACTCCTACCAGTCTTGGCCTCTTGGCTCCATGACACAGGTCCTGCCCCTA GCTAGGAGCCCTACACTCTATCCGCTCAAGACTGGACGTCGAGAAGGGGCAGATCAGGTCCACGTGTGAC CGGACCAGAGGCGACTTCCACGGGCAGCCGTCGGAGTTCTAGTCGTCCGTGACTTTGCCTTACACCAGGC GCTAGGGTCCCCACCTCTTTAGGCGAGCCCCCGCCCCTTAGCGCGGGAACCGCTGTTTGGCTAGCGCATG CTCCCGACTTGGCCGCCGCCGGGCGCAAATGCGCATGCTCAGAGCCGGCAGCACTGAGACGCGGGGCACT CGGGGTGGGCAGCCCGCAAGCGCGTGCTTGTGGGGCGGGGCCGCCTACGCGCGTGCGCAGAAAGTCCAGT GCGGTCCAGCGGAGAGCCGAAGGGAGAGGTGCGGTGCGGCGCGCCGAGGTGGGTGCGCGGCGGGGGCGTG GCGGCTGGACGCGGGGTGTGGGGGTGTCACTGCCACGGTGCGCTGACCGGCGGCACGGATGTACTGAGGG AGGGGGGCACGGGTTGTGCTCCGTGTCTTGGTCGCTGCGGGTTAGGGGGCTCCAGAGTCTAATGCTGCGT GCGTGACGTGGGCCCCGGAGTCAGAACCCTTTGGGCAGGCCCGGCTTTCCTCGATCATCCCTGCCTTCTC TGGGCTCTGTGTCGTGGGCAAGAACTGCCTCTGTGTCAACCTTCCCTTGGGGCACTTTCCGTCCCTGCGG AATAGGAAATCCTCTTTCTGTATTCGTTCGAAATTGCAGCAGTGTTCATATACCTTTGCCCTCGGAGACC TGCAACAACCCAGGCCCTTCACTTGCCAAAGATGACACAACTGCCCACCTGAAGCTTAGGGACTTTCCCA GCCTGAGAGCCCTATCCAGCCAGTGTCCCTGACATGCCTTGGTCACCTGTGCCTTAGCTTGGCCCTGTGC TTCAGGAGTTTTGAATAACAGCTGGAGGGCAGAGCTGGCACCACACGGGGTGGGCTGCACATGACAGAAG TCAGTCTGCTGGGTGCTGGCAGCCTGACCACAGATTTTCCTTGACAGACCTGACTCAGTTCAGAAATAGT GCTCTGGCCTTCTTGGATGGGGACTCACTGGGCTAACTTTAAAGGCCAAAGATTGTCTAGCCCAGCCCGG ACATATGCAGGCATAGGTTGGCCATTTGGGCGGTTGTGTTGCTGTCTTTCCAAGGTAAATAAAGGATTTT CCTTGTCTCACCATGTTCTGCTGGAAGGTCAGCAAGCTACTCCCAGGCAGTTGGTACAACTGTCCCATGG CATGCGTTGCCATGTCATCTACTTGGGGACAATGACTCCATTCTCATTTTCACACCCCTGTCCCCAATAG GCACCTGTCATTGTTCACTGAATTGTTGTGGAGATGGCTGTATTTGTGTTCTCTCAAGCTCTATCTGATG TCAGTTCTGGGTTGGTGTCAAGACCAACCCAGACGACAAATGGTTAAGAAATTTCAAATATTTGATAGTG ACCATTCATATTGAGTGCTATGAGGGCTCTGTGTGCTTCCTTGATGATTAATCCTCACCTAATCTCCCCA GTACTCAGATAAAGACTTAGGGTAGTGACAGCTGTATTTAGAATCTAGTCTGCGTGTGGATAGGCCTGGA GCCACATCGTGTTGTCCCTGTTCCCCCATGGTCTGGCATTCAGTTAGCCAGGCACTCTCTTAAGTGCTTG TTGGAGGGAATAAAGATGCCACTTTTTGAATGCTACATGTCAACTAGTGAACTAGCAATGTAGTGGCTTT TGGCTGGATTAAGGGTGACCTTTTGGGTTTGCAGAAGTACTCTAGTAATCTAGGTAGGGTTTTCATAACA ACAATCAAATTTACCTCCCTGGGAGTGCAAACTCACCTGCCTGTGTTCCCTACTAGCTCTGAGTAACCAC CCTTGCCAGGACTGTCTGGCAAGTCTCAGTGACCTTGGGCACACAGCATTGCTCCTAATCCTTATTAGAG ACTGAGCCAACCTCTGAAGACATAGTAAAGTGTTTAAAGTACACAAGACAATGAAGTGGGTTGTAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6830466 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6830466 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008357 same experiment
	EMAGE:21060 same embryo
	EMAGE:21061 same embryo
	EMAGE:21062 same embryo
	EMAGE:21064 same embryo
	EMAGE:21065 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source