EMAGE mouse gene expression spatial database

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EMAGE:21070

Nudt15 nudix (nucleoside diphosphate linked moiety X)-type motif 15 ( MGI:2443366)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007037. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007037_01	euxassay_007037_02	euxassay_007037_03	euxassay_007037_04

euxassay_007037_05	euxassay_007037_06	euxassay_007037_07	euxassay_007037_08	euxassay_007037_09

euxassay_007037_10	euxassay_007037_11	euxassay_007037_12	euxassay_007037_13	euxassay_007037_14

euxassay_007037_15	euxassay_007037_16	euxassay_007037_17	euxassay_007037_18	euxassay_007037_19

euxassay_007037_20	euxassay_007037_21	euxassay_007037_22	euxassay_007037_23	euxassay_007037_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21070_wholemount_strong.wlz

21070_wholemount_moderate.wlz

21070_wholemount_weak.wlz

21070_wholemount_possible.wlz

21070_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21070_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9923
Entity Detected:	Nudt15, nudix (nucleoside diphosphate linked moiety X)-type motif 15 ( MGI:2443366)
Sequence:	sense strand is shown >T9923 TCAGCCAAATGCTATGGCCGCTAACGCAGAGCCGAGGCGGCGGCCAGGAGTTGGGGTCGGCGTGGTGGTG CTGAGCTGCGAGCATCCTCGCTGCGTCCTTCTGGGGAAAAGGAAAGGATCGTTTGGAGCCGGCAGTTTCC AGCTTCCTGGGGGCCATTTGGAATTCGGTGAGACCTGGGAAGAATGCGCTCAGAGAGAAACCTGGGAAGA AGCTGGGCTTCACCTGAAAAATGTGTGCTTTGCCTCCGTGGTAAATTCTTTTGTTGAGAAGGAGAATTAC CATTATGTTACCATATTAATGAAAGGAGAGGTGGATATGACTCATGATTCGGAGCCGAGGAATATGGAGC CTGAAAAGAATGAAAGTTGGGAGTGGGTTCCATGGGAAGAATTCCCTCCCTTAGACCAGCTTTTCTGGGC TCTCCGCTGTCTAAAAGAGCAAGGTTATGACCCATTTAAAGAGGACCTGAACCACCTGGAAGGGTACCGA GGAGAGCACCTTGAAAGGACCACGAAGACTCCTTGATTAAAAACAAAGTCTTCAGGAAATGAAAACTTCT GTTTCAGGACAACTCGATTTTATCCATAGAGCCACGTGGTCACCAGTTTATCTGCAGTCTCCTGATATAA CTCAAGAACCTTAAGAAACTCTTCCTGGGGTGTGCCGCTGAATTGTCCTGGTGGCATAGAATAAGGTGTG TAAGGAGCTTGCAGTTCATTATCTGCACTTCTGCATGGCTTCCGCTGTCCGCTGCATGGAGGCGAGCGTA ATAGATTGCCTTGTGCTAGTTCAAACAGAGGTCGGAACTCAAATATGGCCATTCAAGTTCAAAAGCAAGG CGCCTACTGCCAAGTTCATCAAGCTTGATGTTTTCCTTTTCCCTTTCAGCCACTTGAAAAGAACTTTTAA ATGACAATTCATGTAACTAGAGAGCTTATGAAAAGCAGTAGGCTACATTCCCCTGGTGTCTGAGAGACCC CGTGTAAGTGTGAGAGTAAGCTGTGGCTCCCGCCACTAACGGCCAGGTCCTCTGCATCTCCCTTTCACAG CCTGAGACTGGGGTCCTTGTGTGCTGGGATCACACAGGGCAATGCATCGAATCACCTACTGGGGGCAGGG GGGTCCCCAGAGGTCAGGAACTCCAGAACATCACAGCCAAAGGTTGGTTGGCTGCTTTCTCCCCCTAGCA AATTGTAAGCTTTTACTGTAAGAGATTGAACAGTGAAATGTCTCCGAGGACTGCATTGTGTGCTGCCTGC TGTCAAAGCAGAGGGTGTTCTGGCTTGAGAGAGGACTTTGGGTTTAAGAGAATCGTGTTCTTGGCCTGAG GACCGCATCGTGCTTAGCTAACGTTAAGAGCTAAAGCTTCTGACCAGCCCATTCATGCCGATCAGCAGCC AGGCTTGGGTTGCATGGACCCTCATCTTTCTCTGGTCAGGCACAGCATAGCTTTCTGCTGATGTAGTTTC TGTTACTGAGCAGTGACTGGCCCTTCTGCTGTGCAGGCCCCTGCTGAGCTCTTCATGTGAGGTGGTTTCT CACCAGTGCCATCTCTGTCCTGCACAGGTGCCATGTGGGTCCTTTACTTGTGGTTCCTGTTCTTTACCCA GTATTCCTGTTTCACATGGGTCAATTTTTGATCAGGGCAAAAACATCAAAGATAGTTGGAGAGTGCTGGC CGCACCTCCGGTGCACAGCCCAGGCAGATGGTTGATGTCAGCTGTGCCTGCACAGCCCAGTTTTTTATAA TGGCCAGATATCACTAATTTCTTCATTTAATCTCATATATCTCAGGCTGGCTTCCAGGTCACTTTGTAGC TAAGGATAACCTTGAACTTCCAGTCTTGCCTCAGCTGCCCTGATGCTGATTTAAGCTACATCGCCCACCC CTCTCTAAGCGTGCACAGTAGTTGCCCGTGTGTGGCTGGTTAGTTTCCTGGGTTACTTTGTTTCAGTACT TAGGGTTGAACTTGGGGCTTGGCACATGACAGGCAAGTGCCATAACAGACTAAATGCAGAGGCAGTTAGA GAAGCCAGCTTCCTTCTGTCAGGTCAGACATTGAAGAGATTTGCCAAAATCTAAAATGACTTTCTCACTG AAGAGTTCATAAAAACTATGTTAACATGTACCTGGTTTTTACTATATGGTCAATAAACTTCCCTAACTTT AATTTCTAACATTAAATATTATTTCATAGCTTACAAAACTAAAACCTGACTTTAAGGTTTTATAAAGGCA TCCAAACCTAAGGGGACCTGGACATTTAGCTCAGTGCTAGGCCACCTGCTACGCTCCGTTCAGTCCTCAT CTCCAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30302091 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30302091 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007037 same experiment
	EMAGE:21066 same embryo
	EMAGE:21067 same embryo
	EMAGE:21068 same embryo
	EMAGE:21069 same embryo
	EMAGE:21071 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source