EMAGE mouse gene expression spatial database

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EMAGE:21080

Egln1 EGL nine homolog 1 (C. elegans) ( MGI:1932286)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013630. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013630_01	euxassay_013630_02	euxassay_013630_03	euxassay_013630_04

euxassay_013630_05	euxassay_013630_06	euxassay_013630_07	euxassay_013630_08	euxassay_013630_09

euxassay_013630_10	euxassay_013630_11	euxassay_013630_12	euxassay_013630_13	euxassay_013630_14

euxassay_013630_15	euxassay_013630_16	euxassay_013630_17	euxassay_013630_18	euxassay_013630_19

euxassay_013630_20	euxassay_013630_21	euxassay_013630_22	euxassay_013630_23	euxassay_013630_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21080_wholemount_strong.wlz

21080_wholemount_moderate.wlz

21080_wholemount_weak.wlz

21080_wholemount_possible.wlz

21080_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21080_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6646
Entity Detected:	Egln1, EGL nine homolog 1 (C. elegans) ( MGI:1932286)
Sequence:	sense strand is shown >T6646 CAGGATAAACGGCCGAACGAAAGCCATGGTTGCTTGTTACCCAGGCAACGGAACAGGCTATGTCCGTCAC GTTGATAACCCAAATGGAGATGGAAGATGCGTGACATGTATATATTATCTAAATAAAGACTGGGACGCCA AGGTAAGTGGAGGTATTCTTCGAATTTTTCCAGAAGGCAAAGCCCAGTTTGCTGACATTGAACCCAAATT TGATAGACTGCTGTTTTTCTGGTCTGACCGGCGTAACCCTCATGAAGTACAGCCAGCATACGCCACAAGG TACGCAATAACTGTTTGGTATTTTGATGCAGATGAGCGAGCGAGAGCTAAAGTAAAATATCTAACAGGTG AGAAAGGTGTGAGGGTTGAACTCAAGCCCAATTCAGTCAGCAAAGACGTCTAGTGGGGCCTTGGGTCCGG CAGTACCCACGTCACCTACAGCCTCTCAGTTGCCTTCTGTGGACTCGTGGACAGGATGGACAGAGAGACA CCTGCCTGGTATTTCAGCTGGGAGCCAGGCGACTTCGCCGGGTGTCATCCAACAGAGGGCTCCATCTGCT GGGACTGTACTGTGGGGTCAGCTCCAGATCTGTGACTGCTCTTGGCTGCTGACCCAAGAGGAGACGCTGT CGGAGGAGAGTAGCTTTTCCATCTGGACACGAAACAAGGGCCCTTTGTAGGAATTTCTTCAGTCTTCTAT TTTGCCAGACCTGTCACCTAACTGAGTTCATTTCATCTCTTTTTTATATCAAGTTTTGAATTCGGGGAAT TTTTGTATTAGGTACAATTTATCAAAACTGAATTAAGAAAAAAAAATTTACAGTATTATTCTCAAAATAA CATCAATCTATTTTTGTAAACCTCTTCATGCTATTAAATTTTGCCCTCAAGGCCTCCTGCGATGATTGTT GCCAGTGAGTGACGACGTGTTGCTTCTGCCTGAACGTAAAGGACGGGCGGGCGCTGTGTCCCAGCCCGAG TGCACGAGGTTTTTCTTGGCCCGTCTCTCAGTGATTCCAACCTGTAAAGGTCACTGCTCTCGCGCTTCGA CCGACCTAACAGTAGATGGTTGCCACTGGCACTCAACTAACTCAACATAGTTACAAGAGGAAACAAGCCA CAGGAGAGGGTTTGTCTCTTCAGTTAATTTTTTTAAAGCGAAGTGACGGGCACTAAATGAACTCGGGGCT CTCCCTCAGCTTCGGGTTCCTGAGACAAAGGGCTTTCTTCTGCGGCAGGTCTAGCCTGCCTACAGCCGTG TCCCACTGCCGCAGGTTTCCTTGTGGCTTCTCCGTAGTTTTGACTGTGCTTCCAGACCCTTCCAGGTCAG GGCTGTGTTCTTGTGGCAGGGCACCTGGTGGACCCAGGCACGTGAATGTGGTATGTGGTTGTAGCCTCAA TCGTGGCCATCGGCTCCTTGGACAGCCACGAGCCATTTTCATACCCAATAATGAAAGCTGTGTGCTAGCT TAGAAATCAAAGGGGGTGTAAAAGCACACATTCTTTGTTTTATGGGTTTTTCTCTTTTTAGAGGACAGAG GGACAACCACACGAGGCTGCCAGACTCCTGTCACCTCTACAGTCCCCTTAGAAAGCCAGAGTTTGCACAG ATTGTGGGTATAACTCCTGTCCCCTTAGGTGTTCTATCTCCGACCTTGATCTTTGCCAAAATGTGTGTAT GCAGAACTATTTCTGTGTATTTTCCTTGACACCCGTCTTAGCACCTGTGTAGTTTGTATCCGGTTAGAAA CCTTTTCTATGGAAAGCTCAGTAATTCTTATTAAGAGATTGCTATTGTTCATGTAAAACATGAAAACAAC CAAGTAGAGCCGTGTGTGGATGAGGGCCCACTCAGCACTGTGCTTGCTTGAGGGGCTCTCGGCAGGAAGT CTCCTTCTGACCCATATCCGCTGACCACACCTCTCCAGCAAGTGCCTCTGCCGCTGGCCAGCTCAAGGTT TGCCCACCTGGCCCCGAAGCACCGTGTTTCGGAGTTGGGAGGAACTGTTTGGCATTGTTGGCAGAAGGTG TGATTGCCTGGAGCAGCAGCCTTTTAAATTCTGGAGACCCTGTAGTCCTTTGTATCTCAGACCTTTACTG ATGTACCAGGTCCCAGATTCTGTGGCAGGGGATGGGGTGGGGTGTGCTTGCCAGACGAAATTTAAATTAT CTATCTTTTGGGAAGTGTGTGCTTTCCTGGAGGTCACTGTGAAAACAAACAAACAAATCAGGACCGTTAA CCCCTTAATGCCCACTTAAACTCAATTTCATGTTAGGACTCTTGTTTAAAACCATTTGTGGCCTGTATGT GTTCATCCTGGTTAGAGAGAAAGCTTTATGACGCTGTTTCTGTTCAACACATTAACCAGCTGTGGAACAG CCCTTTTTGCACGACAGGCAGGGCACTTCAGGATTCGCAGAGAGACTCGTGTGGTTTGGAAGTGGTATTT CCTATGAAAGCCTCTCACGTTGCTTGTAAAGCTAATCTAATTAAAAAGATGTATAAATGTTCTTGAAAAA AATCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3600175 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3600175 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_013630 same experiment
	EMAGE:21078 same embryo
	EMAGE:21079 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source