EMAGE mouse gene expression spatial database

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EMAGE:21133

Erp29 endoplasmic reticulum protein 29 ( MGI:1914647)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001731. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001731_01	euxassay_001731_02	euxassay_001731_03	euxassay_001731_04

euxassay_001731_05	euxassay_001731_06	euxassay_001731_07	euxassay_001731_08	euxassay_001731_09

euxassay_001731_10	euxassay_001731_11	euxassay_001731_12	euxassay_001731_13	euxassay_001731_14

euxassay_001731_15	euxassay_001731_16	euxassay_001731_17	euxassay_001731_18	euxassay_001731_19

euxassay_001731_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21133_wholemount_strong.wlz

21133_wholemount_moderate.wlz

21133_wholemount_weak.wlz

21133_wholemount_possible.wlz

21133_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21133_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

meckel's cartilage

weak

regional

weak expression: see section 2 3 4 5 6 7 8 5 6 7 8 9

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4140
Entity Detected:	Erp29, endoplasmic reticulum protein 29 ( MGI:1914647)
Sequence:	sense strand is shown >T4140 GCATCTCGCTCTGGCGGTGGCCGCTTGCGTTCATGCGCGCCGCCGGCAGGACGGCCCTCCCCGTGACGTG ACCGCCCACTCGGAGCGCTCCGCCTGTAGCTTGCCTGTCTCTCCCGCTCCCACCATCCGGCGTGATGGCC GCCGCCGCCGGGGTGTCTGGCGCTGCTTCTCTCTCCCCACTGCTGTCCGTGCTCCTGGGCCTGCTGCTCC TCTTTGCTCCGCACGGCGGCAGCGGCCTACACACGAAGGGTGCCCTTCCCTTGGACACAGTCACTTTCTA CAAGGTCATTCCCAAAAGCAAGTTCGTCTTGGTGAAGTTCGACACCCAGTACCCCTATGGAGAGAAGCAA GATGAGTTCAAGCGTCTGGCTGAGAACTCAGCCTCCAGCGAGGAGCTCTTGGTGGCAGAGGTGGGGATCT CAGACTATGGCGACAAGCTGAACATGGAGCTGAGTGAGAAGTACAAGCTGGACAAGGAGAGCTACCCAGT CTTCTACCTATTCCGGGATGGGGACTTGGAGAACCCTGTGCTGTACAATGGGGCAGTTAAGGTTGGAGCC ATCCAGCGCTGGCTAAAGGGGCAGGGGGTCTACCTGGGCATGCCTGGGTGCCTGCCTGCATACGATGCCC TGGCAGGCGAGTTCATCAAGGCCTCCAGCATAGAGGCCCGACAGGCCATCTTGAAACAGGGACAGGATGG CCTCCTAAGTGTGAAGGAAACAGAGAAGAAGTGGGCTAGCCAATACCTGAAGATCATGGGGAAGATCCTG GACCAAGGTGAGGACTTTCCAGCCTCTGAGATGGCCCGGATCGGTAAGCTGATAGAGAACAAGATGAGTG ACAGCAAGAAAGAAGAGCTACAGAAGAGCTTAAACATCCTGACGGCCTTCCGGAAGAAGGAGGCTGAGAA GGAGGAGCTGTGACCAAGCACAAGGGTTCCCCAGGGTTTGCCAGGGGCAGGAAAGGAGAGATCTACCTGC CAGCTGTGGGACCCCTGTGGGTGGAAGGGGCAGTGGAGCAATGCAGGCCTGAGCCAGAAGTCTGTGCCCC GAGTGTGCCTGGACATTGATGCTGCTGAGATCATACTTAGGACACCCCCTGAGCCAGTCTGTGGAGAGCC TGTGTGCTCAGTCAAGATGGCTGGTGAGATGCCCCCCGCAGGAGTTGGTGCTATAGAGGTAGGGACTACT GCCAGGTCCTTGATGAGTGTAATTCTGATCAAATAAAAAGTCTGTTTTGGTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4481563 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4481563 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001731 same experiment
	EMAGE:21132 same embryo
	EMAGE:21134 same embryo
	EMAGE:21135 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source