EMAGE mouse gene expression spatial database

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EMAGE:21140

Marveld3 MARVEL (membrane-associating) domain containing 3 ( MGI:1920858)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003994. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003994_01	euxassay_003994_02	euxassay_003994_03	euxassay_003994_04

euxassay_003994_05	euxassay_003994_06	euxassay_003994_07	euxassay_003994_08	euxassay_003994_09

euxassay_003994_10	euxassay_003994_11	euxassay_003994_12	euxassay_003994_13	euxassay_003994_14

euxassay_003994_15	euxassay_003994_16	euxassay_003994_17	euxassay_003994_18	euxassay_003994_19

euxassay_003994_20	euxassay_003994_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21140_wholemount_strong.wlz

21140_wholemount_moderate.wlz

21140_wholemount_weak.wlz

21140_wholemount_possible.wlz

21140_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21140_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 6 7 8 9 1 2 4 5 6 7 8

4th ventricle lateral recess

moderate

regional

moderate expression: see section 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

esophagus

weak

regional

weak expression: see section 1 2

stomach fundus

strong

regional

strong expression: see section 1 2 3 4

kidney calyx

moderate

regional

moderate expression: see section 6 7 6

trachea

weak

regional

weak expression: see section 2

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5005
Entity Detected:	Marveld3, MARVEL (membrane-associating) domain containing 3 ( MGI:1920858)
Sequence:	sense strand is shown >T5005 GGGAAACTTATGGTTGGTTGTCGCCCTCGGATCGTTTCCGGGCGGGGACAGGAATCCGGCCATGAAAAAT ACTTCGGGGCACCGTGAGCCCCGGACCCGTCCTAGAGAAAGAGACCCGGACCGGCGCCCGCACCCGGACC GAGACCACCACGTCGAGCGGTCGCGGGACCGAGGCGGGGACAGGCACAGGGAGAGGAACGGTGACGTGAG AGGAAACGGGGACCGTCGGGCGGGCCGGGAACAGAGAACGGACCGAGACCAGCGCCAGGACAGACACAGG GACGCGGGCCATCGCGCATCCGAACAAAGAGCCTTGGAGAAGTCCCGTCAAAGCCGTGCGCGGCCCGAGC CCTGGGGACCCAGTTGGGACGCAGCCCCGACTCCGGGACCCGCACCCTGGGGGCCCCCAGAGCTGTCGCA GAAGCATGGCCTCGGGCGCCGCGGGTTGGAGAGCGAACGGGCTTCGGAAAGATACGTGCCCACCTACTCC GTGCCTGCTCTCCAGGAAGAGGAATATTACCAGTCGGAAGCGGAGGGACTCCTGGATTGCCACAAATGCA GATACTTGTGCACAGGGAGAGGTGTGGTGCAGATAATGGAGGTGATTTTGAATGCCATGGTTCTCATATG CATCGTGGCTTCCTATTTTGTCCTGGCCGGATTCAGCGCCAGCTTTGCCAGCGGCAGCGGCTTCGGAAAT AACTATTACTCACCTTTTGAGGGCACCGAGTTGGAGCAGGTTCGGCAGCTGGACCAGCAGTACACGATCC TGCGGTCGCCCCTGATCTACGGCGGCGTGGCTGTCTCCCTGGGGCTGGGCGTCCTCACTATGGGCGTTTT ACTGCAGGGAGCTAAGAGTTTAAGGAAGCTGCCCAGGAGATGGCTGCTGCTGGAGGCCGCCTTCAGCCTC CTGGCCGCGGTGGGCTACTGCGTCGCCATTGGCTTTTACCTGTATGCGGCGTTGCGCATCAATTCCACCG ACACTTGCAAAACCAGAGAAAGAGTTTACGCCCGCAAGGGCCTCACCTGGATGAACTGCCAGCTGGCGGG CACCGATGGGGCAGCAGCCACCTTCGCTTGTTTTTTAGTGATTTTATACGCGGCCAGCGTGGTGATGGCC CTGCGGGCCTACCGAGAACAGAAGCACTACAAAGACAGCCAGGAACAACACAGAAATTACCGTGATGTTC CAGAATACCTGTGGTCCGGGACGCTTTAAGGAGAGCTGGAACCTCCTTTGTTCCTCCCCTACAAGCACAA CGTGATTTCCCATACTTGATTCCTAAGCTCCCTCAGTGCTCAGGCACCTGAGCAGAGTAAAGTTGTGAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5249917 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5249917 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003994 same experiment
	EMAGE:21141 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source