EMAGE mouse gene expression spatial database

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EMAGE:21204

Emcn endomucin ( MGI:1891716)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012119_01	euxassay_012119_02	euxassay_012119_03	euxassay_012119_04	euxassay_012119_05

euxassay_012119_06	euxassay_012119_07	euxassay_012119_08	euxassay_012119_09	euxassay_012119_10

euxassay_012119_11	euxassay_012119_12	euxassay_012119_13	euxassay_012119_14	euxassay_012119_15

euxassay_012119_16	euxassay_012119_17	euxassay_012119_18	euxassay_012119_19	euxassay_012119_20

euxassay_012119_21	euxassay_012119_22	euxassay_012119_23	euxassay_012119_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21204_wholemount_strong.wlz

21204_wholemount_moderate.wlz

21204_wholemount_weak.wlz

21204_wholemount_possible.wlz

21204_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21204_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

other

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30748
Entity Detected:	Emcn, endomucin ( MGI:1891716)
Sequence:	sense strand is shown >T30748 CAGATGGAACACCTCCCGAAGGAACGACCAAAAGTGACGTATCCCAAACATCTTTGGTGACAACAATTAA TTCACTGACAACACCAAAACATGAAGTGGGAACAACAACTGAAGGTCCCCTAAGGAATGAAAGTTCCACT ATGAAAATAACAGTGCCAAATACTCCAACTTCAAATGCTAATTCAACGCTACCAGGTTCCCAGAACAAGA TTACTACTCAACTACTGGATGCCTTGCCGAAAATCACTGCAACACCCTCTGCATCTCTAACAACTGCACA CACCATGTCACTGCTTCAAGATACTGAGGATAGGAAGATTGCAACCACTCCATCAACCACCCCCTCCTAT TCCAGTATCATTTTGCCTGTGGTTATCGCGCTGGTTGTTATAACACTCTTGGTATTTACTCTGGTGGGTT TGTATCGAATATGCTGGAAGAGAGACCCAGGCACTCCAGAAAACGGAAATGATCAGCCTCAGTCCGATAA AGAGAGTGTGAAGCTGCTCACTGTTAAGACAATCTCTCATGAGTCTGGCGAACACTCTGCACAGGGGAAA ACCAAGAACTGACATCGTGATGGATCCTCCCTACACCTGGGCAATAAATATGCTTCATCTTCAGCTTCTA AACTCCAAGGGTGGAAAGAAGAAAAAGTCCTGAGGAACCAACACAATTTCCAGTCTTCTCACCAGTCTAC ACCAGTCACCTGTCTTAGCAAAGGAATGCCATATCACCACCTGATAGTACCATAAAGAGCTCTGGATATC TCCTGTGTGAGATGTAGAAATCGACCTAGTTTTCAGCGACTTTGCTAGGAGGAACTGTGATCCCCCCAGC CCAGGCAGGTGGGTTCCTGGAAAACATGCCGTAGTTGAGTTTAGACTTTCCAGGTTTTCTTAGCCTCGAT CCGTGGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3485788), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 37716. Forward Primer - name:037716_F_IRAV01-03_H13_Emcn, sequence:CAGATGGAACACCTCCCG; Reverse Primer - name:037716_R_SP6_IRAV01-03_H13_Emcn, sequence:CTCCACGGATCGAGGCTA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012119 same experiment
	EMAGE:21202 same embryo
	EMAGE:21203 same embryo
	EMAGE:21205 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source