EMAGE mouse gene expression spatial database

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EMAGE:21212

Cldn10 claudin 10 ( MGI:1913101)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014336. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014336_01	euxassay_014336_02	euxassay_014336_03	euxassay_014336_04

euxassay_014336_05	euxassay_014336_06	euxassay_014336_07	euxassay_014336_08	euxassay_014336_09

euxassay_014336_10	euxassay_014336_11	euxassay_014336_12	euxassay_014336_13	euxassay_014336_14

euxassay_014336_15	euxassay_014336_16	euxassay_014336_17	euxassay_014336_18	euxassay_014336_19

euxassay_014336_20	euxassay_014336_21	euxassay_014336_22	euxassay_014336_23	euxassay_014336_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21212_wholemount_strong.wlz

21212_wholemount_moderate.wlz

21212_wholemount_weak.wlz

21212_wholemount_possible.wlz

21212_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21212_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 9 0 1 2 3 4 5 6 7 8 9

vibrissa

strong

regional

strong expression: see section 5 6 7 0 1 2

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 8 9 0 1 7 8 9 moderate expression: see section 5 6 weak expression: see section 2

esophagus epithelium

strong

regional

strong expression: see section 4 5

lower jaw incisor

strong

regional

strong expression: see section 2 5

lower jaw molar

strong

regional

strong expression: see section 9 9 moderate expression: see section 0

oral epithelium

moderate

regional

moderate expression: see section 9 0 1 2 3

upper jaw incisor

strong

regional

strong expression: see section 2

upper jaw molar

strong

regional

strong expression: see section 8 9 moderate expression: see section 0

metanephros

strong

regional

strong expression: see section 9 0

left lung

strong

regional

strong expression: see section 6 7 8 9 0 1 2 3 4

right lung

strong

regional

strong expression: see section 5 6 7 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31517
Entity Detected:	Cldn10, claudin 10 ( MGI:1913101)
Sequence:	sense strand is shown >T31517 CCATGACAGGCTGTTCCCTGTATGCAAACAAAATCACAACAGAATTCTTTGATCCTCTCTATATGGAGCA AAAGTATGAATTAGGGGCTGCTCTCTTCATCGGATGGGCAGGAGCTTCTCTCTGCATCATTGGGGGAGTC ATATTTTGCTTTTCAATATCCGACAACAATAAGACACCCAGAATGGGCTACACATACAACGGACCCACGT CTGTCATGTCTTCTCGGACCAAGTATCAAGGCGGAGAAGGAGATTTTAAAACCGCAGGCCCTTCAAAACA GTTTGATAAAAATGCCTATGTCTAAAGAGCTCTGGCAAGCTGCCTCCTGAGTTTTGTTGTGCAAGAGAAC TGTCCTCACAATAGTCCTTCCAAGGCTCTCCTGTAATTACTGCTTAAGCTGTTTTTTAAAAATATAAATT TGAAGAATGTTAATTGGATGTAAATGTTCTTATAGTTATATACTAATCATTTTCTGCTGTGCCTTTCTGT AAGGGAAATAAACAGGTTATTTACAGGGTGTGTATATTTTATACACATAGAAGACATGGGTACCCTTAGT TCATAAGGCTCAGGGAATGGTAGCCACTGGATCCTTAATGTGCTGAGAGCTTCCCTCGTTCCACCCACAG ATCAAACACAAGTGTGCAGACGATGGATGGTGTCGTGTGAAGGGGACAGCGATAATCTCAGACCCCTCAT CCAGTATCCAAGTCAAACAATCACACAACCAATGTGCCATGTCGTTGATTTCTAGTTTCTTGGTTCTGTC CATCAGGGCTGTCATTTGTCATGATGGCCAAAGAAAGGGCTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5356256), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 28020. Forward Primer - name:028020_F_IRAV63-66_P10_Cldn10, sequence:CCATGACAGGCTGTTCCC; Reverse Primer - name:028020_R_SP6_IRAV63-66_P10_Cldn10, sequence:ACCAGCCCTTTCTTTGGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_014336 same experiment
	EMAGE:21213 same embryo
	EMAGE:21214 same embryo
	EMAGE:21215 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source