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EMAGE:21258

Csn3 casein kappa ( MGI:107461)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21258 EMAGE:21258 EMAGE:21258 EMAGE:21258 EMAGE:21258
"Pseudo-wholemount" of euxassay_004880. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004880_01 euxassay_004880_02 euxassay_004880_03 euxassay_004880_04
EMAGE:21258 EMAGE:21258 EMAGE:21258 EMAGE:21258 EMAGE:21258
euxassay_004880_05 euxassay_004880_06 euxassay_004880_07 euxassay_004880_08 euxassay_004880_09
EMAGE:21258 EMAGE:21258 EMAGE:21258 EMAGE:21258 EMAGE:21258
euxassay_004880_10 euxassay_004880_11 euxassay_004880_12 euxassay_004880_13 euxassay_004880_14
EMAGE:21258 EMAGE:21258 EMAGE:21258 EMAGE:21258 EMAGE:21258
euxassay_004880_15 euxassay_004880_16 euxassay_004880_17 euxassay_004880_18 euxassay_004880_19
EMAGE:21258
euxassay_004880_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21258Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21258_wholemount_strong.wlz
21258_wholemount_moderate.wlz
21258_wholemount_weak.wlz
21258_wholemount_possible.wlz
21258_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21258_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6883
Entity Detected:Csn3, casein kappa ( MGI:107461)
Sequence:sense strand is shown

>T6883
TAAGAGGCCGGGCAAACCCTACTGCCAAGCAAGAATTGACTCCAAATAAAGGTGCAATGATGAGGAATTT
TATCGTAGTTGTGAATATTCTGGCATTAACTCTGCCCTTTTTGGCTGCAGAGATACAAAACCCAGATTCA
AACTGCCGTGGTGAGAAGAATGACATAGTGTACGATGAGCAAAGAGTCCTGTACACACCAGTCCGCTCCG
TCCTGAACTTCAATCAATATGAACCTAATTACTACCACTACAGGCCATCTTTGCCTGCTACTGCTAGCCC
ATACATGTACTACCCACTTGTTGTTAGACTACTTCTGCTTAGGTCTCCAGCCCCAATCTCCAAATGGCAA
TCAATGCCAAATTTCCCCCAGTCTGCTGGAGTACCTTATGCCATTCCAAACCCATCCTTTCTTGCCATGC
CTACAAATGAAAATCAGGATAACACCGCCATTCCAACCATCGACCCCATTACTCCCATTGTGTCCACACC
AGTTCCTACCATGGAGTCAATAGTAAATACTGTGGCCAATCCTGAAGCTTCCACAGTGTCTATCAATACC
CCTGAGACTACTACAGTCCCAGTGTCCTCAACTGCAGCATAAAACACTAAGGAAACATTGAAAAAGACAA
TAAAGGTCTGGAACCAAATTTCAACTTGAAACTCCTTCAGGGGAAACTCTGCCATCATTCAAGAGAAAAT
GCAATTTTCACAGATTCAGATCTTTCTCGTCTTCCCTTACCTTTTACATTAACATCACAGTTAATTTTGT
GTTTTGATTCTTAAAGAATAAAGTTAATGAAATGCAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3966924 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3966924 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_004880 same experiment
 EMAGE:21257 same embryo
 EMAGE:21259 same embryo
 EMAGE:21260 same embryo
 EMAGE:21261 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS