EMAGE mouse gene expression spatial database

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EMAGE:21264

8430410K20Rik RIKEN cDNA 8430410K20 gene ( MGI:1925350)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009273_01	euxassay_009273_02	euxassay_009273_03	euxassay_009273_04	euxassay_009273_05

euxassay_009273_06	euxassay_009273_07	euxassay_009273_08	euxassay_009273_09	euxassay_009273_10

euxassay_009273_11	euxassay_009273_12	euxassay_009273_13	euxassay_009273_14	euxassay_009273_15

euxassay_009273_16	euxassay_009273_17	euxassay_009273_18	euxassay_009273_19	euxassay_009273_20

euxassay_009273_21	euxassay_009273_22	euxassay_009273_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21264_wholemount_strong.wlz

21264_wholemount_moderate.wlz

21264_wholemount_weak.wlz

21264_wholemount_possible.wlz

21264_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21264_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1138
Entity Detected:	8430410K20Rik, RIKEN cDNA 8430410K20 gene ( MGI:1925350)
Sequence:	sense strand is shown >T1138 CCTCGAGCCTGTTGGCCTACTGGAGACAGAGGTGCCCCGGGGTCGCAGGTATCTAGCCAGCTTCCAGGAC CAGGTGATTGGAAACCTCAGTGATTTGAACTAAGAGCCAACAAAGCCTTGGGTTTGTGGCACACTCTTCA CATTCTTTGCACAGCTCTTTTTGCTGTTAAAATCCGCACTGGATTTTTAAAAATGAAGCAATTGAAAAGA AAAAGGAAAAGCAATTTTAGTGTTCAGGAGACTCAGACCCTTTTAAAAGAAATTACCAAGAGGAAGGAAG TCATTTTTTCCAAGCAGCTCAACACAACAATAAACGTGATGAAGCGAATGGCCTGGGAGGAGATCGCACA GTGTGTGAATGCTGTCGGAGAAGGAGAGCAGAGGACAGGCACAGAGGTTAAGAGACGTTACCTGGACTGG CGCGCACTCATGAAAAGGAAGAGGATGAAGGCCAACATGAAGCTCGTTGGTTCTGGGTTTCCTCTGCCCA CGTCTGATCTAGATGACTCTCTCACTGAAGACATAGANGAAAAAATTGCCTTCCGAAATGATGCAAATTT TGAGTGGCAGA
Notes:	The probe template was PCR amplified from IMAGE:2135806 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2135806 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009273 same experiment
	EMAGE:21262 same embryo
	EMAGE:21263 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source