EMAGE mouse gene expression spatial database

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EMAGE:21309

Phf13 PHD finger protein 13 ( MGI:2446217)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000058. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000058_01	euxassay_000058_02	euxassay_000058_03	euxassay_000058_04

euxassay_000058_05	euxassay_000058_06	euxassay_000058_07	euxassay_000058_08	euxassay_000058_09

euxassay_000058_10	euxassay_000058_11	euxassay_000058_12	euxassay_000058_13	euxassay_000058_14

euxassay_000058_15	euxassay_000058_16	euxassay_000058_17	euxassay_000058_18	euxassay_000058_19

euxassay_000058_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21309_wholemount_strong.wlz

21309_wholemount_moderate.wlz

21309_wholemount_weak.wlz

21309_wholemount_possible.wlz

21309_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21309_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex ventricular layer

moderate

homogeneous

moderate expression: see section 7 8 weak expression: see section 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 9 0

trigeminal v ganglion

weak

homogeneous

weak expression: see section 4 5 6 7 8 9 4

dorsal root ganglion

weak

homogeneous

weak expression: see section 9 0 1 3 4

renal cortex

strong

regional

strong expression: see section 5 6 8 moderate expression: see section 7 8 9 0 1 3 4 5 7 9 weak expression: see section 6

lung

moderate

spotted

moderate expression: see section 6 7 8 9 0 1 3 4 weak expression: see section 5 5 7 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1606
Entity Detected:	Phf13, PHD finger protein 13 ( MGI:2446217)
Sequence:	sense strand is shown >T1606 TAAAAAACAAAAAGCATATTTTTTTCCCCACAATACAGCCCAAAGTATTTGCCACTGTTACACTGGGTGT CCCAGCTCTGCTGGTGGTGCCCAGTCCAGTAACAGTCCACAGAAGACACTGTCCTTGGGCCCTGTGTGCT GTTCTTCAGATAATGGGTGAAAAGAATGGGCATTCTGCCTTCCATACAGGTTCCTGGGGCAAGGCAAGGT CTGAGTGCTAGAGCACCAGCTTGCAGAGTTTCAGAGGTGTGAACCAGAGCCCTGGTCTGAGTGAGGGCTG GCTCAGCTCCTGGACACTGGTGGTCCTGGGGCAGAGGGAAAGGAGGAAGAGGGCGTTGGCTCTCAAACCA GCATCTCCAGTGCTCATTCTTTCTCCGTAGTGTTTCCTACCCAGTTGTCACGCGTATTCTTTGTTAGCCT GAGACAGAGCAGAGTGGCCAGTGTGTCACCTTTCTTCCCCTTCCTGGAGTACCTTTGTAAGGTCACAGGC ATTCTCCCACATTGTCTTAAGCACCAAAGGAGC
Notes:	The probe template was PCR amplified from IMAGE:1038075 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1038075 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000058 same experiment
	EMAGE:21307 same embryo
	EMAGE:21305 same embryo
	EMAGE:21304 same embryo
	EMAGE:21306 same embryo
	EMAGE:21308 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source