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EMAGE:21322

Ctnnd2 catenin (cadherin associated protein), delta 2 ( MGI:1195966)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21322 EMAGE:21322 EMAGE:21322 EMAGE:21322 EMAGE:21322
euxassay_018872_01 euxassay_018872_02 euxassay_018872_03 euxassay_018872_04 euxassay_018872_05
EMAGE:21322 EMAGE:21322 EMAGE:21322 EMAGE:21322 EMAGE:21322
euxassay_018872_06 euxassay_018872_07 euxassay_018872_08 euxassay_018872_09 euxassay_018872_10
EMAGE:21322 EMAGE:21322 EMAGE:21322 EMAGE:21322 EMAGE:21322
euxassay_018872_11 euxassay_018872_12 euxassay_018872_13 euxassay_018872_14 euxassay_018872_15
EMAGE:21322 EMAGE:21322 EMAGE:21322 EMAGE:21322 EMAGE:21322
euxassay_018872_16 euxassay_018872_17 euxassay_018872_18 euxassay_018872_19 euxassay_018872_20
EMAGE:21322 EMAGE:21322 EMAGE:21322 EMAGE:21322
euxassay_018872_21 euxassay_018872_22 euxassay_018872_23 euxassay_018872_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21322Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21322_wholemount_strong.wlz
21322_wholemount_moderate.wlz
21322_wholemount_weak.wlz
21322_wholemount_possible.wlz
21322_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21322_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50211
Entity Detected:Ctnnd2, catenin (cadherin associated protein), delta 2 ( MGI:1195966)
Sequence:sense strand is shown

>T50211
CATGAAGTGATCACCAAGAACATGGAGAATGCCAAGGCCTTACGGGATGCTGGTGGCATCGAGAAGTTGG
TCGGCATCTCTAAAAGCAAAGGAGACAAGCACTCTCCAAAGGTGGTCAAGGCTGCTTCTCAGGTCCTAAA
CAGCATGTGGCAGTATCGCGATCTGAGGAGTCTCTACAAGAAGGATGGATGGTCACAATATCACTTTGTA
GCCTCATCTTCAACCATCGAGAGGGATCGACAAAGGCCCTACTCCTCCTCCCGCACACCCTCCATCTCTC
CCGTGCGTGTGTCTCCCAACAACCGCTCAGCAAGTGCCCCAGCTTCACCTCGGGAAATGATCAGCCTCAA
AGAAAGGAAGACGGACTACGAGTCCGCTGGCAACAACGCCACTTACCACGGAACTAAAGGAGAACACACC
TCCAGAAAAGACACCATGACAGCTCAAAACACTGGAGTTTCAACTTTGTACAGGAATTCATACGGTGCGC
CCGCTGAAGACATCAAACAGAACCAGGTTTCCACACAGCCTGTCCCTCAGGAGCCCAGCAGGAAAGACTA
CGAGACCTACCAGCCCTTTCCGAATTCCACACGAAATTATGATGAGTCCTTCTTTGAGGACCAGGTCCAC
CACCGCCCTCCAGCCAGCGAGTACACCATGCACCTGGGCCTCAAGTCCACTGGCAACTATGTCGACTTCT
ACTCTGCAGCCCGTCCTTACAGTGAACTGAACTATGAAACGAGCCACTACCCGGCCTCGCCCGACTCCTG
GGTGTAAGGAGCCAGGACACGAGGCACTC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CATGAAGTGATCACCAAGAA; Reverse Primer - name:unspecified, sequence:GAGTGCCTCGTGTCCTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_018872 same experiment
 EMAGE:21323 same embryo
 EMAGE:21324 same embryo
 EMAGE:21325 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS