EMAGE mouse gene expression spatial database

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EMAGE:21384

Cwh43 cell wall biogenesis 43 C-terminal homolog (S. cerevisiae) ( MGI:2444131)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000544_01	euxassay_000544_02	euxassay_000544_03	euxassay_000544_04	euxassay_000544_05

euxassay_000544_06	euxassay_000544_07	euxassay_000544_08	euxassay_000544_09	euxassay_000544_10

euxassay_000544_11	euxassay_000544_12	euxassay_000544_13	euxassay_000544_14	euxassay_000544_15

euxassay_000544_16	euxassay_000544_17	euxassay_000544_18	euxassay_000544_19	euxassay_000544_20

euxassay_000544_21	euxassay_000544_22	euxassay_000544_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21384_wholemount_strong.wlz

21384_wholemount_moderate.wlz

21384_wholemount_weak.wlz

21384_wholemount_possible.wlz

21384_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21384_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2334
Entity Detected:	Cwh43, cell wall biogenesis 43 C-terminal homolog (S. cerevisiae) ( MGI:2444131)
Sequence:	sense strand is shown >T2334 TCGAGGCCAGATTCGTCGACATGCGTCGACATGCAGACTTTGGGCCAAGCACCAGGGATCACACCTGGGG GATTATGGTGCTGTCTCGGTACCCGATTGTCAGATCGGAACATCACCTTCTTCCGTCGCCAGAGGGCGAG ATCGCACCAGCCATAACCATGACAGTTAACGTCTCCAACAGACTGGTGGATTTTGTGGTGACACACTTTG GGAATCATGAAGATGACCTTGACAGGAAGCTACAGGCTATTGCAGTTTCAAAACTGCTGAAAAATTGTTC AAATCAAGTGATATTTCTGGGTTATATCACTTCAGAGCCTGGTTCCAGAGATTACATACAACTCACAAAA CATGGCAACGTGAAGGATATTGACAGCTCAGATGGAGACAGATGGTGTGAGTACATCATGTACCGGGGTC TGATCAGGTTGGGCTATGCAAGAATCTCTCATGCAGAACTGAGTGACTCTGAGATTCAGATGGCCAAATT CAGGATCCCGGATGATCCCGCCAATTACAGAGACAACCAGAAAGTCGTCATAGACCACC
Notes:	The probe template was PCR amplified from IMAGE:1166769 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1166769 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000544 same experiment
	EMAGE:21388 same embryo
	EMAGE:21387 same embryo
	EMAGE:21385 same embryo
	EMAGE:21386 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source