EMAGE mouse gene expression spatial database

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EMAGE:21422

Timp1 tissue inhibitor of metalloproteinase 1 ( MGI:98752)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000782_01	euxassay_000782_02	euxassay_000782_03	euxassay_000782_04	euxassay_000782_05

euxassay_000782_06	euxassay_000782_07	euxassay_000782_08	euxassay_000782_09	euxassay_000782_10

euxassay_000782_11	euxassay_000782_12	euxassay_000782_13	euxassay_000782_14	euxassay_000782_15

euxassay_000782_16	euxassay_000782_17	euxassay_000782_18	euxassay_000782_19	euxassay_000782_20

euxassay_000782_21	euxassay_000782_22	euxassay_000782_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21422_wholemount_strong.wlz

21422_wholemount_moderate.wlz

21422_wholemount_weak.wlz

21422_wholemount_possible.wlz

21422_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21422_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

meckel's cartilage

moderate

regional

moderate expression: see section 3 4 6 7 8 9 1 2

lower jaw molar

moderate

regional

moderate expression: see section 8 9 0 1 3 4 5 7 8 9 1

upper jaw molar

moderate

regional

moderate expression: see section 6 7 8 9 0 1 3 7 8 9 1 2

axial skeleton

moderate

regional

moderate expression: see section 2 3 4 5 6 7 weak expression: see section 8 9 2 3 4 5 8 9 0 1 2

chondrocranium

weak

regional

weak expression: see section 2 3 4 5 6 7 8 1 2 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2299
Entity Detected:	Timp1, tissue inhibitor of metalloproteinase 1 ( MGI:98752)
Sequence:	sense strand is shown >T2299 TGGCCTCGAGCCAGATTCGGATCCTTGCTCCTAGAGACACACCAGAGATACCATGATGGCCCCCTTTGCA TCTCTGGCATCTGGCATCCTCTTGTTGCTATCACTGATAGCTTCCAGTAAGGCCTGTAGCTGTGCCCCAC CCCACCCACAGACAGCCTTCTGCAACTCGGACCTGGTCATAAGGGCTAAATTCATGGGTTCCCCAGAAAT CAACGAGACCACCTTATACCAGCGTTATAAGATCAAGATGACTAAGATGCTAAAAGGATTCAAGGCTGTG GGAAATGCCGCAGATATCCGGTACGCCTACACCCCAGTCATGGAAAGCCTCTGTGGATATGCCCACAAGT CCCAGAACCGCAGTGAAGAGTTTCTCATCACGGGCCGCCTAAGGAACGGAAATTTGCACATCAGTGCCTG CAGCTTCTTGGTTCCCTGGCGTACTCTGAGCCCTGCTCAGCAAAGAGCTTTCTCAAAGACCTATAGTGCT GGCTGTGGGGTGTGCACAGTGTTTCCCTGTTTATCTATCCCTTGCAAACTGGAGAGTGACACTCAC
Notes:	The probe template was PCR amplified from IMAGE:1122897 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1122897 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000782 same experiment
	EMAGE:21423 same embryo
	EMAGE:21425 same embryo
	EMAGE:21426 same embryo
	EMAGE:21421 same embryo
	EMAGE:21424 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source