EMAGE mouse gene expression spatial database

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EMAGE:21487

Oma1 OMA1 homolog, zinc metallopeptidase (S. cerevisiae) ( MGI:1914263)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001138_01	euxassay_001138_02	euxassay_001138_03	euxassay_001138_04	euxassay_001138_05

euxassay_001138_06	euxassay_001138_07	euxassay_001138_08	euxassay_001138_09	euxassay_001138_10

euxassay_001138_11	euxassay_001138_12	euxassay_001138_13	euxassay_001138_14	euxassay_001138_15

euxassay_001138_16	euxassay_001138_17	euxassay_001138_18	euxassay_001138_19	euxassay_001138_20

euxassay_001138_21	euxassay_001138_22	euxassay_001138_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21487_wholemount_strong.wlz

21487_wholemount_moderate.wlz

21487_wholemount_weak.wlz

21487_wholemount_possible.wlz

21487_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21487_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 3 4 5 6 7 weak expression: see section 2

nasal capsule

weak

regional

weak expression: see section 1 2 3 4 5 7 8 9 0

esophagus

weak

regional

weak expression: see section 3 4 5 6

oral epithelium

weak

regional

weak expression: see section 2 3 4 5 6 7 8

liver lobe

weak

homogeneous

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 7 8 9 0 1 2 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4280
Entity Detected:	Oma1, OMA1 homolog, zinc metallopeptidase (S. cerevisiae) ( MGI:1914263)
Sequence:	sense strand is shown >T4280 AGCAAAAGAAAGATGAGCCTCCTTTATGGACTGCAGTCTACCAGGATAAATCGGTTTCTCTCTGGAGTGA ATAACCTGGCCAACAGGAGACAGTGGACCCCCCCAGCAAGCTGTCCACTGGCACCAAAGCTCCGAGCAGT AAATGCATACTGGGGACTGAACACAGTCAGTCATTGTCATTCAGTGACCTTACTGCCTAGAAACTTTCTT TTCTGTAGGACTCTCAATCACAAAAAATCAAGATGCCTCTCAAGTGCCCAAAGCAAGGAATTGGGGGTGC TTACCTACAGATGTACTGTGCGGGGTGATTCTGTTCTAAGACAAGGAGCAAGGAAAGTGGCTGGCGTTCC TGCTCTCGCGGCCTCCTGTTCTCCAAGCTGTCCTGCCGTAATAGAGGCCCGGAGTTTCCGTACATCTGCA AGGGTTCAGGCTGCCCCAGTCCCTCTCTTGCTGCTCATTCTGAAGCCAGTGCAAAAGCTCCTTGCTATCA TCGTGGGCAGGGGCATAAGGAAATGGTGGCAAGCACTTCCCCCTAACAAGAAGGAGCTATTTAAAGACAG CGTGAGGAAGAACAAGTGGCGGCTGCTTCTTGGTCTGAGTGCATTTGGACTGCTCTTTGTAGTGTTTTAT TTCACTCACCTGGAAGTGAGCCCTGTCACCGGAAGGAGTAAACTACTGTTAGTGGGCAAAGAACACTTCC GACTTCTGTCAGACCTGGAATATGAAGTATGGATGGAAGAATTTAAGAACGATCTGCTGCCTGAGAGAGA CCCCCGCTACCTGACTGTGAAAGAAATGGTTTATCATCTGACACAGTGCAATCGAGACGTCCCGGGGATC TCGGAGACCAACTGGGTGGTTCATGTGGTTGACTCCCCAGCCGTCAATGCCTTTGTGCTCCCAAATGGAC AAGTGTTTATTTTCACCGGGCTTCTGAATAGTGTGACGGACGTGCACCAGCTGTCCTTCCTCCTGGGCCA TGAGATCGCACACGCAGTCCTGGGGCACGCCGCAGAAAAGGCTAGCTTGGTTCATTTACTGGATTTCCTT GGTATGATTTTTCTCACAATGATTTGGGCCATTTGTCCCCGAGACAGCTTGGCAGTTTTGGGCCAGTGGA TACAGTCAAAGTTGCAGGAGTACATGTTTGATCGACCATACAGTAGAACATTGGAGGCCGAAGCTGACAA AGTTGGACTACAGCTGGCTGCAAAGGCATGTGCCGATGTAAGAGCTAGTTCAGTGTTTTGGCAGCAGATG GAGTTCTCAGAGAGTCTTCATGGCTATCCCAAGTTGCCAGAGTGGCTGTCGACACATCCTTCTCATGGGA ACCGAGCTGAGTACTTGGATAGACTCATACCACAGGCTCTCAAACTCCGGGAGGTCTGCAATTGCCCGCC ACTCTCTGGCCCAGACCCTCGGCTATTGTTCAGGCTTACTGTGAAGCGTTTTCTTGAAGACTCGGAGAAA GAGGACCTAAACATCACTGTCAAAAAGCAGAAAACGGATGCTCTCCCCATGCAGAAGCAGGAGCAGATAC CACTGACGTATGTCCTAGAGAAGAGAACTGCAGGCTGAACGGAAGTGGGTGAATCGTGGGCTCCATGAGC AGCACAGTGGACCTTTTCCTTATTACGCAACTTTTAAAAATGATGTTTTCAAGGAAAAAAAAAAAAGAAT GTTCAAAGGCCAAGTCACTTATATTATAGATATTATCTGGATTTTTCTAGGTTTTTATCTTTCATGAAAT ATTAATGTGTTTCCACCTATTTTAAAATACTGCCAATGAGGAAAACATCACAGAATAAATTGATATTATG CATTTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4504835 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4504835 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001138 same experiment
	EMAGE:21491 same embryo
	EMAGE:21488 same embryo
	EMAGE:21486 same embryo
	EMAGE:21489 same embryo
	EMAGE:21490 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source