EMAGE mouse gene expression spatial database

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EMAGE:21532

Nap1l3 nucleosome assembly protein 1-like 3 ( MGI:1859565)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002914_01	euxassay_002914_02	euxassay_002914_03	euxassay_002914_04	euxassay_002914_05

euxassay_002914_06	euxassay_002914_07	euxassay_002914_08	euxassay_002914_09	euxassay_002914_10

euxassay_002914_11	euxassay_002914_12	euxassay_002914_13	euxassay_002914_14	euxassay_002914_15

euxassay_002914_16	euxassay_002914_17	euxassay_002914_18	euxassay_002914_19	euxassay_002914_20

euxassay_002914_21	euxassay_002914_22	euxassay_002914_23	euxassay_002914_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21532_wholemount_strong.wlz

21532_wholemount_moderate.wlz

21532_wholemount_weak.wlz

21532_wholemount_possible.wlz

21532_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21532_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 4 5

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 7 8 9 0

trigeminal v ganglion

moderate

regional

moderate expression: see section 3 4 5 6 7 8 9 8 9 0 1 2 3

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 6 9 0

dorsal root ganglion

moderate

regional

moderate expression: see section 9 2 7 8 9 weak expression: see section 0 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4284
Entity Detected:	Nap1l3, nucleosome assembly protein 1-like 3 ( MGI:1859565)
Sequence:	sense strand is shown >T4284 AGACGGTTCTGAGTCCGGTACTGCGTCACTGGACTGCAGCAGGTCTCGGCGCAGGTCCATTGCGGCCTGA CAAGCCTCGTAGCTAAGGCTCCAGGCTGCTTCCCCTGCTGCGGTTTCCTGCGCGCCACCTCTGTGACCCC GCCCCCATCGCGCCACTGCCACAGCTGCTGCAGTCCGTGTTGCCACTGGATCCCGGCCGCCGCCGCTGCA GTCTCCGCCGCCTGACCTTCTCCGGCCTTCGCCCCTGCTGCCACCGCCCCCCACCGCCGCCGCCACTGGA GCATCCGTGAGTCCTTTTCTGTCCGTCTCTGGGTGCACGATCTCTGCTGTTGTCACCACCTTAAATAGGT GTACGGGGCTAACAGTATGGCAGAAGCGGATCCTAAAATGGTCACAGAACCTGGTGCCCATGGGGTTGCT GAAGAGGCGATGGCTAGCACAGCTTGTGATTCTGGGGATGAATCTGACAGCAATAGCTCTAGCAGTACCA ATAGTTGCAGCAGCAGCGGCAGCAGCAGCAGCGGCAGCAGCAGTAGCAGCAGCAGCAGTAGCAGCAGCAG CAGCAGCAGCAGCAGTAGCAGTAGCGGTAGCAGTGGCAGCAGCAGCAATGGCAGTCATTTGAACCGAAAG AAGAGGGTACCTGAGCCTTCCAGAAGGGCCCAGCGACGTCCCTCCGGGAAACTTTTCTTGGATAAGCTGC CCCAAGCCGTAAGAAATCGGGTGCAGGCACTCAGAAATATTCAGAATGAGTGTGACAAGGTAGACACCTT GTTCTTAAGGGCAATTCATGATCTTGAAAGAAAGTATGCTGAACTCAATAAGCCTCTATATGATAAGCGT TTTCAGATCATAAATGCAGAATATGAGCCTACAGAGGAAGAATGTGAATGGAATTCAGAAGAAGAGTTCA GTGGTGATGAAGAAATGCAGGATGACACACCTAATGAAATGCCACCCTTAGAGGGTGAGGAGGAAGAAGA AAGCTGTAATGAAAAAGCTGAAGTGAAGGAAGAAGGAACACATGTTCCAGAAGAAGTTCCTGAGGCAAAA GTTGAAGAAGAGGAGGCTCCCAAAGAAACTCCTGAGGTGAAAACTGAAGAAAAAGACATTCCAAAAGAAG GTGCTGAAGAAAAAGCTGAAGAACAGGAATCCTCTAAAGAAATTCCTGAGGTAAAAGGTGAAGAAAAAGC AGACTCTACGGATTGTATAGATATAGCTCCTGAAGAAAAAGAAGACGTCAAAGAAGTTACCCAGGCAAAT ACAGAAAATAAGGATCAACCTACAGAAGAATTTACACCAAGGGCTCCAGCAAGAGAGGCTCAAAAAAGGG TCCCTGAGACAAGGCCTGAAGAAGGAGTCAATATTAAAAGGGCTCGAAAGGGAAAACCTAAGAAAGAAGA TCCTAAAGGTATTCCTGACTACTGGCTGACTGTTTTAAAGAATGTTGATAAGCTTGGGCCTATGATTCAA AAGTGTGATGAACCCATTTTGAAGTTCTTATCTGATGTGAGCCTGAAGTTCTCAAACCCTGGCCAGCCTA TTGGTTACACTTTTGAATTTCATTTCCTACCTAACCCATACTTCAGAAATGAGCTCCTGATGAAGACATA CATAATAAGGTCAAAACCAGATCACTACGACCCGTTCTTCGCGTGGGGATGGGAAATTGAAGAGTGTAAA GGCTGCAAAATAGACTGGAGACGAGGAAAAGATGTTACGGTGACAACCACCCGGAGTCGCCCTGGTATTA CAGGGGAAATTGAAGTCCAGCCAAGAGTGGTTCCTAATGCATCCTTCTTCAATTTCTTCAGTCCTCCTGA GATTCCTTTGATTGGGAAGCTGGAACCAAGAGAAGATGCTATCCTTGATGAGGACTTTGAGATTGGTCAA ATTTTGCATGATAATGTCATCTTGAAGTCAATCTATTACTTCACAGGAGAAATCAATGATCCCTACTACC ATGACTTCAGGGATTATGGAAATAGGAAGTACTACAAGTAGAAAGGGCAAGCTGAAAAATCTTTCAAGAA TCCTTAAAAGTTAAGCAAGCAGAAGTGAAATAAGAGTCATCTAAACTTCTAAAATAGAAAACTTGCCCCA TAACCTGAACAGTATTACTCCTTATAGTCTGGTGTTTTCATTTTTTCCTGGTAATGTCCTAAAAAGTGTC TTAAGCTTCTGTTTATTCTATCTTGCCTGTTGTATTGTAATATTCTTCAAAATATGTAAACTGCTGTTAA TTTCCAAAGCATGTTTTGTTTGGTGCTACAGTGTTGGTTTTGTGAAGTCAGTTTTTGTCATGTTGCTATT AGATTGTACCTGTGAAAATTGTCAGATTTATTAGAGAAATCCTTGCCTGAATAAGCAACATTTTAAATGC CAATATACGTGTGTTTTCTCTTTCTTTTTTTACAGCCCAGAAGACTAAAGACTTAAATCTGCTTGCACTA GATGTGCCTTCATTACTTTGCCATAGAAATGTAGTAATAATAACAACTGAAACACTCTATTGTGAAATGT AGTTGCTTGAAAAAAAAAGATGAGAATATATTTAAAAATGTCAGGATCAAGTAGATTTTTGTGGGTTTGT TTATGTGATTTATTGGTCTGTTAGCAAAAGTATTGTTTACCACTAAGGTGTTAAATATGGTATAGTATTT TAAAATTTTTCTTTGTTGTATATTGTGTAATTGAAAACTAAAGAAACCGTATTTTCTTTGTAATGTGTTC TACATTGCCTTTTAAAAATTATTTCATCATTTGATACATTATCATGTTCTTTTATTGTACCTGGAGTAAA TATCAAATAAACTGCCATGCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4505496 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4505496 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002914 same experiment
	EMAGE:21530 same embryo
	EMAGE:21531 same embryo
	EMAGE:21534 same embryo
	EMAGE:21529 same embryo
	EMAGE:21533 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source