EMAGE mouse gene expression spatial database

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EMAGE:21535

Slc35e4 solute carrier family 35, member E4 ( MGI:2144150)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001091. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001091_01	euxassay_001091_02	euxassay_001091_03	euxassay_001091_04

euxassay_001091_05	euxassay_001091_06	euxassay_001091_07	euxassay_001091_08	euxassay_001091_09

euxassay_001091_10	euxassay_001091_11	euxassay_001091_12	euxassay_001091_13	euxassay_001091_14

euxassay_001091_15	euxassay_001091_16	euxassay_001091_17	euxassay_001091_18	euxassay_001091_19

euxassay_001091_20	euxassay_001091_21	euxassay_001091_22	euxassay_001091_23	euxassay_001091_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21535_wholemount_strong.wlz

21535_wholemount_moderate.wlz

21535_wholemount_weak.wlz

21535_wholemount_possible.wlz

21535_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21535_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

weak

regional

weak expression: see section 1 4 not examined expression: see section 2

telencephalon marginal layer

weak

regional

weak expression: see section 2 3 4 5 6 7 8 9 0 1 2 3

medulla oblongata basal plate

weak

regional

weak expression: see section 9 0 6 7

facial vii ganglion

weak

homogeneous

weak expression: see section 4 5 0 1

glossopharyngeal ix ganglion

weak

homogeneous

weak expression: see section 7 8 9

trigeminal v ganglion

weak

homogeneous

weak expression: see section 3 4 5 6 7 8 8 9 0 1 2 3

ventral grey horn

weak

regional

weak expression: see section 1 2 3 5 6

dorsal root ganglion

weak

regional

weak expression: see section 9 0 1 7 8 9

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4194
Entity Detected:	Slc35e4, solute carrier family 35, member E4 ( MGI:2144150)
Sequence:	sense strand is shown >T4194 CTGCAGCCAGTCCGAGCTCGCGATTCCGCGTCCGCCGGCCTATCGGCGTCTATTCCCGGTCCAGTAAAAG GCTAAAGGGGGCTGGGGGAGGGGAGCGACGGCTGCAACCTCCGTTCCCACGCTCGGAATATTATCGACCC TGCCAGACCTGGTCCGGGCCTGAGCACCAAACACGCAGGCGGTCGGGGTCAGTCAGTGACCTCATCCTGA CAGTCTGGGGACCAAAGATGGAAAAAGACAAACGGATTCGGTGTGAAAGCATCGCGTGAGCTTAGGGCGC GGAGAAGAGAGAAGGCCTCCCCTGGTGCGGGAGGTGCCCAGGGCTTACGAGGCCTGGGTGGACACCAGCC AGTCCGGCATCTTAGCAGCCACGACCTTCTTCCCTGACCAGAATTATCGGCTGGCTCAGAGTCTAGCACC TGAAGTGTTGCCCTCTCCTGTCCGAGGAATCAGGACCAGGCTCAGCGACTCCCTGGAAAGCCTGCCTCCT GCCCCAGCCCCACTGGTGCGGATGTGCCGCTGCCCACTGGAGCACCATGAAGGCATGATGACCTCAGCCG AAGCAGTTGCTGTGGCTGGAAGTGCCCAGGAGCATGGCCGCCCCAAGTGGCCTCCGGACAAACCCCAGGT CCTTGGGCAGCCTGCTCCGGCGAGAGTGGTTGTGGCAGCACTGGTGTGGCTGTTGGCGGGAGCCAGCATG TCAAGCCTCAACAAGTGGATCTTCACAGTGCATGGCTTCGGACGGCCCCTGCTGCTTTCAGCACTGCACA TGCTGGCAGCCGCCCTGGCATGCCACTGGGGGGCCCAGCGCCCCGTGCCGCACAGCATCCACCGTCGAGT GCTGCTGCTCAGCCTCACCTTCGGTACCTCCATGGCGTGCGGCAACGTGGGCCTGAGCACTGTCCCCTTG GACCTAGCACAGCTGGCCACCACCACCACACCACTGTTCACAATGGCCTTGTCTGCGTTGCTGCTTGGCC GAAGACACCATCCTCTGCAGTTTGCTGCCATGGGCCCGCTCTGCCTAGGGGCTGCATGTAGTCTGGCTGG AGAGCTCCGGGCACCTCCAGCTGGTTGCGGCTTCTTGCTGGTGGCCACCTGTCTGCGAGGCTTCAAGTCT GTTCAACAAAGTGCTCTGCTGCAAGAAGAGAGGCTGGACACGGTGACCCTGCTGTATGCCACTTCTTTGC CCAGTTTCTGCCTGCTGGCCAGTGCAGCACTGGTGCTGGAGGCTGGCACAGCCCCACCGCTGCCTCCCAC TGACTCTCGCCTCTGGGCCTGTGTCCTGCTGAGCTGCTTCTTGTCCGTGGTCTACAACCTGGCCAGCTTC TCCTTGCTGGCCCTCACATCTGCCCTCACTGTCCACGTACTGGGCAACCTCATGGTCGTGGGCAACCTCA TCCTGTCCCGGCTCCTGTTCGGCAGCCACCTCAGCGCGCTCAGCTACCTGGGCATCACCCTCACCCTTTC AGGAATGTTTCTTTATCACAACTGTGAGTTGGTGGCCTCCTGGGCTACCCGATGGGGGTTGTGGAACAGG GACCAGCCTGGCAAAGGTCTTTGAGACCTGGGGGAGCTCATGAGCTCTACCTGGACAGCCCCTGCCTGAA TCTAACCTTGGACAATGGCCATGAGGAAAGGGAGAGGTGCTATGTCTGCCAGGCCCAGGAGAGAAGGCAC CTTCTGGAAGGGCTGCCTGGGAGGCTACACACCAGGGACTTTGAGAGAGACCCACCTCCTGTCCCTGCTT GCCTCACCTCTCACAGCAAACCCTGCCACTGGATGGTGGGTCCAAGTCTGGCACGTGTGCTTGCCAGAGG AACCAGTGTCAACGACTATGATGAAGGTTGCTGGGCAAACTTAGGGAACCTCAAAGTGTTAGGATGAAGA CAATGCTCGGTAGTCACACAATCTTTCCTGGGGGGCGAGGGAAGTGGAGGCTGTGAGGGTCACCAGAGCA GGCTTCGTCATGGCTGGGCTCCCCTAAAGATGAGGTAGCCTGTGCCAACTTCAGGTAGCTACTGCGGCTT CACTCTGGTGCCTCCACCCCTGGATTTGGGCCTTCCATCCCCAATAAAGTATTTTTTCTTGGGAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4488513 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4488513 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001091 same experiment
	EMAGE:21538 same embryo
	EMAGE:21539 same embryo
	EMAGE:21536 same embryo
	EMAGE:21537 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source