EMAGE mouse gene expression spatial database

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EMAGE:21541

2810432L12Rik RIKEN cDNA 2810432L12 gene ( MGI:1914313)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001059_01	euxassay_001059_02	euxassay_001059_03	euxassay_001059_04	euxassay_001059_05

euxassay_001059_06	euxassay_001059_07	euxassay_001059_08	euxassay_001059_09	euxassay_001059_10

euxassay_001059_11	euxassay_001059_12	euxassay_001059_13	euxassay_001059_14	euxassay_001059_15

euxassay_001059_16	euxassay_001059_17	euxassay_001059_18	euxassay_001059_19	euxassay_001059_20

euxassay_001059_21	euxassay_001059_22	euxassay_001059_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21541_wholemount_strong.wlz

21541_wholemount_moderate.wlz

21541_wholemount_weak.wlz

21541_wholemount_possible.wlz

21541_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21541_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 4 6 7 weak expression: see section 5

facial vii ganglion

moderate

homogeneous

moderate expression: see section 5 6 7 8 1 2

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 8 9 0 1

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 4 5 6 7 8 9 0 1 8 9 0 1 2 3

vagus x ganglion

moderate

homogeneous

moderate expression: see section 0 1 9

dorsal root ganglion

moderate

regional

moderate expression: see section 0 1 2 3 4 8 9 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T228
Entity Detected:	2810432L12Rik, RIKEN cDNA 2810432L12 gene ( MGI:1914313)
Sequence:	sense strand is shown >T228 CGAGACCAGCCAGCCTGGGCTACAGCAGCGGTGGCCTGGGCTACAGCGGCGGTGGCCTTGGCAAGCCAGG ACGTAGCTGACGCCTCCACGGGGTTTGACTTCATGCTACTGCCCTGAGATCCAGGATGGTTTGTTCTTGG GACCAGACATGACCAAAAGTTGACACCATGACCACGTCAACCTCTCCAGCTGCCATGCTTCTCCGGAGGC TTCGGCGACTCTCCTGGGGCAGCACAGCTGTGCAGCTCTTCATTCTAACCGTGGTGACATTTGGCTTACT GGCCCCGCTGGCCTGCCACCGGCTCCTCCACTCATATTTCTATCTGCGTCATTGGCATCTAAACCAGATG AGCCAGGACTTCCTGCAGCAGAGCCTGAAGGAGGGGGAAGCTGCCCTTCACTACTTTGAAGAGCTGCCCT CTGCCAATGGGTCTGTGCCCATCGTCTGGCAGGCCACTCCCCGCCCCTGGCTGGTG
Notes:	The probe template was PCR amplified from IMAGE:2650271 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2650271 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001059 same experiment
	EMAGE:21540 same embryo
	EMAGE:21542 same embryo
	EMAGE:21544 same embryo
	EMAGE:21545 same embryo
	EMAGE:21543 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source