EMAGE mouse gene expression spatial database

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EMAGE:21550

Pola2 polymerase (DNA directed), alpha 2 ( MGI:99690)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001087. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001087_01	euxassay_001087_02	euxassay_001087_03	euxassay_001087_04

euxassay_001087_05	euxassay_001087_06	euxassay_001087_07	euxassay_001087_08	euxassay_001087_09

euxassay_001087_10	euxassay_001087_11	euxassay_001087_12	euxassay_001087_13	euxassay_001087_14

euxassay_001087_15	euxassay_001087_16	euxassay_001087_17	euxassay_001087_18	euxassay_001087_19

euxassay_001087_20	euxassay_001087_21	euxassay_001087_22	euxassay_001087_23	euxassay_001087_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21550_wholemount_strong.wlz

21550_wholemount_moderate.wlz

21550_wholemount_weak.wlz

21550_wholemount_possible.wlz

21550_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21550_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

weak

regional

weak expression: see section 5 6 7 8 2 3 4

thymus primordium

moderate

homogeneous

moderate expression: see section 3 4 5 6 7

submandibular gland primordium

weak

regional

weak expression: see section 8 9 0 1 9 0 1 2

foregut-midgut junction

weak

homogeneous

weak expression: see section 5 6 7

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 1 2 7 8 9

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

rest of cerebellum ventricular layer

moderate

homogeneous

moderate expression: see section 3 4 5 6 7 8 9 0 1 6 7 8 9 0 1 2 3

midbrain ventricular layer

moderate

regional

moderate expression: see section 9 0 1 2 3 4 5 6 7 8 weak expression: see section 8

stomach

weak

homogeneous

weak expression: see section 5 6 7 8 9 0 1 2

hindgut

weak

homogeneous

weak expression: see section 5 6 7

midgut

weak

homogeneous

weak expression: see section 7 8 9 0 1 2 3 5 6 7 8

lower jaw incisor

weak

regional

weak expression: see section 2 3 4 7 8

upper jaw incisor

weak

regional

weak expression: see section 2 3 4 6 7 8

liver lobe

weak

homogeneous

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

kidney calyx

weak

homogeneous

weak expression: see section 0 1 2 3 4 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4655
Entity Detected:	Pola2, polymerase (DNA directed), alpha 2 ( MGI:99690)
Sequence:	sense strand is shown >T4655 CCACGCGTCCGGTTTGGCGGGTTTTTCCTGTGCTGTGCTCCTCACGGAGAAGCTAAGGTGTAGTCCTCGG AAGCAAGTTTTCTCTCTGGATCGAGACCTCTCGAGGGCTTTTGAAGTATTTCTCTGTGACCTGGTGGCTG GGGTCGTTTGAGGGCTTAGGCCAGGTGGTCTCGTGACACGTCCCTGTCACCTTCCGGTAAAGCGAAAGGA TAAGCGTCTGCAGGGTAGAGCTGAGGTCGGGGCCCGGCGGCAGGCGCCCGGCCACTCCCGGGTCACCATG GCCGTGTCCACCCAGCAGCTGGCGGAGGAACTACAGATCTTCGGCCTGGACTACGAGGACTCCCTGTTGG AGAAACTGGCAGAGCTGTGTGTTCTATACCGACAGACTGAGGACGGGATGGTCAGTGAGCTCATCGCCTT CTGCACCAGTGCGGGGAAGACATGCCTTACTGTAGACATCCTGAACTCTTTTGAATACGAGGTTCTGAAC AAAAAACTCTCCAAAGCCTGGCACAGTGCCTCCAAAGACAGTGGGCATGCAGGAACCAGAGACATTGTTT CTATACAAGAGCTAATTGAAGCAGAGGAGGAAGAGGAGACCCTCCTAAGCTCTTATACCACACCCTCTAA GGGTCCTCTCAAGCGAGTTAGCAGCACCCCAGAAACCCCCCTAACCAAAAGGAGCGTCGCTGCTCGCAGC CCACGTCAGCTTCTCTCACCGTCAAGTTTCTCCCCGAGTGCTACCCCCTCCCAGAAATACACCTCCAGAA CCAACCGGGGAGAAGTGGTGACCACCTTTGGCTCCGCACAGGGCTTGTCTTGGTCCGGGAGAGGAGGCTC TGGAAGCGTCAGTCTAAAGGTCGTGGGGGACCCAGAGCCACTCACTGGCAGCTACAAAGCGATGTTTCAG CAGCTCATGGGTGTTCGAGAAGTTCTGACCAGTAAGATCGAAGAGCTCGGCAGCGAGCTGAAGGAGCATC ACAAGATCGAGGCCTTTACCCCTCTCCTGGTCCCAGCGCAAGAGCCTGTTATTCTCCTGGGTCAGATTGG CTGCGACAGCAACGGGAAGCTGAACAGCAAGTCCGTGATTCTCGAGGGGGACCAGGAGCACTCCTATGGC GCGCAGATCCCAGTGGACCTATCGGAACTCAAAGAGTATTCCCTCTTCCCTGGACAGGTTGTAATTATGG AAGGATTCAACACCACTGGTAGGAGACTTACTGCCACCAAACTCTATGAGGGCGTGCCACTTCCGTTCTA TCAGCCTACTGAAGAGGAGGGAGCTTCTGAGCAGACTATGGTGGTAGTTGCCTGCGGGCCTTATACCACC TCCGACAGCATCACCTATGACCCCCTGCTTGATCTGATCGCCATCATCAACCGTGACCAGCCAGATGTCT GCATCCTGTTCGGACCTTTCCTGGATGCTAAACATGAACAGGTGGAGAACTGTAAGTTGACAAGCCCATT CGAAGACGTTTTCAAGCAATGCCTGAGAACAGTTATCGAAGGAACGAGAAGCTCCGGCTCCCACCTTGTC TTTGTCCCGTCTCTGAGGGATGTCCATCATGAGCCAGTATACCCACAGCCGCCTTTCACCTTCTCCGAGC TGTCTCGAGAGGACAAAAAGCGAGTGCAGTTTGTGTCAGAGCCCTGCAGCCTCTCCATAAATGGAGTGAT GTTTGGTTTGACATCCACGGACCTGCTGTTCCACATCGGGGCTGAGGAGATCTTTAGCTCTTCCGGGACG TCAGACAGATTCAGCCGAGTCCTTAAGCATATCCTGACGCAGAGAAGTTACTACCCACTTTATCCACCCC ACGAGGACATGGCCATAGACTACGAGAACTTCTATACCTATGCCCAGCTGCCGGTCACCCCCGATGTCTT CATAGTGCCGTCCGAGCTGAGATACTTTGTGAAGGACATCTTTGGCTGTGTCTGTGTGAATCCTGGGCGG CTCACGAAAGGACAAGTGGGCGGCACCTTTGGCCGCCTCTACCTCAGGAGGCAGCCCAAGGCCATGGACG GTGGGGGCAGGCAGGGCCTGAGCGTGGCAGCGCAGGTGGTCAGAATCTGAGTCCTCAGCCTGGTTGCCTG GCCTCACAAACACCAAGAGCCCAGGTATGGCTCTCTGGTGGCCAAACTGTTGGAGTACCTGACAAGGATC TGGCCACAGAGACAGGACTGTAGGGCTGGGCCGTCCAGTCACGGCTTCCCACTGTCCCTCACCCCCATGC AGCGTGGCTCTGATCTGAATTACGCCCTGCTTCTCTCCTGCTTCATGAGGTAGATGTACAGAAGTGAGCC ATCTATGGACACAAAGGCAGGAAAACTGACTTTTTTTTGTATGAATCAAATACAGCGTGTGGAGAAATAA AAATTTTTTTAGTATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5011204 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5011204 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001087 same experiment
	EMAGE:21551 same embryo
	EMAGE:21547 same embryo
	EMAGE:21549 same embryo
	EMAGE:21548 same embryo
	EMAGE:21546 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source