EMAGE mouse gene expression spatial database

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EMAGE:21555

Gnpnat1 glucosamine-phosphate N-acetyltransferase 1 ( MGI:1858963)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003054_16	euxassay_003054_17	euxassay_003054_18	euxassay_003054_19	euxassay_003054_20

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View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21555_wholemount_strong.wlz

21555_wholemount_moderate.wlz

21555_wholemount_weak.wlz

21555_wholemount_possible.wlz

21555_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21555_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4846
Entity Detected:	Gnpnat1, glucosamine-phosphate N-acetyltransferase 1 ( MGI:1858963)
Sequence:	sense strand is shown >T4846 CTGACGCGCCGGGACTGGAGCGTGGTCCGCTCCAGTGCGACTTTACTGGAAAGATGAAACCTGATGAAAC TCCCATGTTTGACCCAAGTTTGCTCAAAGAAGTGGACTGGAGTCAGAATACAGCTATTTTTTCTCCAGCC ATTTCTCCAACGCATCCTGGAGAAGGCTTGGTTTTGAGGCCTCTCTGTACTGCAGACTTAAATAAAGGTT TTTTTAAGGTATTGGGTCAGCTGACAGAGACTGGCGTCGTCAGCCCGGAACAGTTCATGAAATCTTTTGA GCATATGAAGAAGTCTGGAGATTACTACGTTACAGTTGTGGAAGATGTGACCCTAGGACAAATTGTGGCT ACAGCAACTCTGATAATAGAACATAAATTTATCCATTCATGTGCTAAGAGAGGGAGAGTAGAAGATGTCG TCGTTAGTGACGAGTGCAGAGGGAAGCAACTCGGCAAACTGTTATTATCAACTCTTACTTTGCTAAGCAA GAAATTGAACTGTTACAAGATCACCCTTGAATGTCTACCACAAAATGTTGGTTTCTACAAAAAGTTTGAC TATACAGTATCTGAAGAAAATTACATGTGTCGGAGGTTCCTAAAGTAAAAACCACCATCAAGAGGTTAAT GCTACAAAGCTTAAATAACTGTTTTAGAGTTCATTTTTGTTGCTGCAGCCCAGTGAGCTCCCTAACTACT AGACTGAAGTACACTGTGGTGTGCCAGTGTGTAGTAACCTGGGAGGATGCTGGGAATACAAATAAAGGGG AGGATTTTTAACCAAAGGAATATATTTTCAACTTGAATCTTTTCTTGCATTGTATTTTTCTAAAGTTTGT CTTCATTTCTTGGTAGTCAAGTGTATGGGTAGTAAACATGTCTGCTCTATGCTGCTCATTTTTAAATATA TGTATATACATATATATTGAATAAGGGTGTTTCTTACCATAAAATTATTTTTGTCTCATACATCTAAATA GACTTAAGGTGCACCATTTCTCATTGGACACTTCTGTTATCATGACCACCAGATACATATTTGTTGTTGA TCCTAACCCTGCAGTCTTTTTAAAGCAACAAAAGAAAAATGGGACAAGGTACAACAGCTTAAATAACGAT TTACTAAGTGTAAAGTTTTGTGCAGATACTGTACCACCTGCCACCTGTCACATGGTATTAGAATTATCCA GGGTCCTTTTCATTTGTCAATGAGCAGCTCCCAGAGCTGCTACCCTGGTTTCCGTACCATTGACATTTGA AGCCTTTGAGTCACAAAAAAATGTTCAGTACTTCCTAAATTTAGTATAGCTTTAAAATGATGGTAGCTTT TCTATTTAATGTGATTATAATGGTACTATTATTCTGATCCCTGTCCTGTCTGTTTTATTTAAATGATTTT TAAAATAGAATCAGATACTTTATTAATAGTGGTTCTAATCTTTTACATTCCATGTTATTACATTAAACTT GTTTATATGAATAGTCTTTCATGTTAGAATCTTGTCTTGTGTTTTTCTTTTTTGTTGAGACAGGGTGCCC AGGATATCCTGGAACCCCTCACACATGTTCTAAGAACTGGTATTCCAGGCATGTACCACCATGATCAGCT TCAGTCTATTAGGTTAAGACCTGGCTCTTAATACCCTAGGTTTAAGTATCTTTGTACTTCAATTGGACTT TGGATTTTCCTGCAGTCATTAGGTTTGTCTTTGATTTTCAGAGTTCTTTAGTTCTGTAACCATGACACAG CTTCATTAGGTTTTATCTATGTTAAAAAAAAGCCTTTTCCCCAAAGTAAAATTTAGCACTTTATTTTTTA ACATTAAACTCTTTGGTAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5065641 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5065641 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003054 same experiment
	EMAGE:21553 same embryo
	EMAGE:21556 same embryo
	EMAGE:21557 same embryo
	EMAGE:21554 same embryo
	EMAGE:21552 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source