EMAGE mouse gene expression spatial database

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EMAGE:21584

Thoc5 THO complex 5 ( MGI:1351333)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003792_01	euxassay_003792_02	euxassay_003792_03	euxassay_003792_04	euxassay_003792_05

euxassay_003792_06	euxassay_003792_07	euxassay_003792_08	euxassay_003792_09	euxassay_003792_10

euxassay_003792_11	euxassay_003792_12	euxassay_003792_13	euxassay_003792_14	euxassay_003792_15

euxassay_003792_16	euxassay_003792_17	euxassay_003792_18	euxassay_003792_19	euxassay_003792_20

euxassay_003792_21	euxassay_003792_22	euxassay_003792_23	euxassay_003792_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21584_wholemount_strong.wlz

21584_wholemount_moderate.wlz

21584_wholemount_weak.wlz

21584_wholemount_possible.wlz

21584_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21584_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7032
Entity Detected:	Thoc5, THO complex 5 ( MGI:1351333)
Sequence:	sense strand is shown >T7032 CCACGCGTCCGGGCTGGAAGGCGTGAGGAGCATTCTGCCTTTTCACCTGGAAGCACAATGTCATCAGAAT CCAGCAAAAAACGGAAACCCAAAGTGATCCGGAGTGATGGAACCCCAACTGAAGGGAAACGGAACCGGTC TGACACGGAGCAGGAAGGCAAATACTACAGTGAGGAGGCTGAAGTTGACCTGCGGGACCCTGGCAGAGAC TATGAGTTGTACAAATACACTTGCCAGGAACTACAAAGGCTGATGGCCGAGATCCAGGACCTGAAGAGCA AGGGCAGCAAGGACGTGGCCATTGAAATTGAAGAACGAAGGATCCAGAGCTGTGTGCACTTCATGACTCT AAAGAAGCTTAACCGATTAGCCCACATCAGGTTAAAGAAAGGAAGAGACCAGACCCACGAGGCCAAGCAG AAAGTCGATGCCTACCACCTACAGCTACAGAACCTGCTCTACGAAGTGATGCACCTGCAGAAGGAGATCA CCAAATGTCTGGAGTTCAAGTCAAAGCATGAAGAAATTGATCTGGTCAGCTTAGAGGAATTTTATTCAGA GGCTCCACCAAGCATCAGCAAGGCCGAGATCACCATGGGAGACCCTCACCAACAAACATTGGCACGTTTG GACTGGGAGCTGGAGCAGCGGAAAAGGCTGGCAGAAAAGTACCGAGAATGTCTGTCTAACAAGGAGAAGA TCCTGAAGGAGATTGAGGTGAAGAGGGACTATCTGAGCAGCCTCCAGCCTCGCCTCAACAGCATCATGCA GGCTTCCCTGCCAGTGCAAGAATACCTGTTCATGCCGTTTGACCAGGCGCACAAACAGTATGAGACTGCC AGGCACCTGCCGCCTCCCCTCTATGTGCTCTTTGTGCAGGCCACTGCATACGGGCAGGCCTGTGATAAGA CGCTCTCCGTGGCAATTGAAGGCAGTGTGGACGAAGCCAAAGCTCTTTTCAAGCCCCCTGAGGACTCCCA AGATGATGAGAGCGACTCAGATGCTGAAGAGGAGCAGACGACGAAGCGGCGGCGGCCCACTCTCGGGGTT CAGTTGGATGACAAGCGCAAGGAGATGCTAAAAAGGCACCCACTGTCTGTCCTGCTGGATCTGAAGTGCA AAGACAACAGTGTGCTTCATCTGACTTTCTACTATCTCATGAACCTCAACATCATGACAGTGAAAGCCAA AGTGACGACCGCCGTGGAGCTGATCACCCCCATCAGTGCAGGAGACTTACTGTCTCCTGACTCGGTCTTG AGCTGCTTGTATCCGGGAGATCATGGGAAAAAAACTCCAAATCCAGCCAATCAGTATCAGTTTGACAAAG TTGGCATCCTGACTTTGAGAGACTATGTACTTGAGTTAGGTCACCCCTACTTGTGGGTACAGAAGCTGGG TGGCCTCCACTTCCCCAAAGAGCAGCCCCAGCAAACAGTGATGCCTGACCACTCACAGAGCGCCAGCCAC ATGGAGACCACCATGAAACTGCTGAAGACCAGGGTACAGTCCCGCCTGGCTCTCCACAAACAGTTTGCAT CTCTGGAACATGGCATTGTGCCCGTCACCAGCGATTGCCAGGACCTCTTCCCTGCCAAGGTTGTTTCTCG CCTGGTAAAGTGGGTGATAATTGCCCATGAAGATTACATGGAGCTGCACTTCACTAAGGACATTGTGGAG GCAGGACTGGCCGGAGACACCAATCTCTACTACCTGGCGCTAATTGAAAGGGGAACAGCTAAACTTCAGG CTGCTGTGGTACTAAACCCTGGCTACTCCTCCATCCCACCGGTTTTCCGACTCTGTCTAAACTGGAAAGG GGAGAAAACCAACAGCAACGATGACAATATTCGGGCTATGGAGAGTGAAGTCAACGTGTGCTATAAGGAG TTGTGTGGCCCTCGGCCCAGTCACCAGCTCCTGACCAACCAGCTGCAGAGACTGTGTGTGCTGCTGGACG TCTACCTGGAGACCGAGAGCCACGATGACAGCTTCGAGGGCCCCAAGGAGTTTCCCCAGGAGAAGATGTG CCTGCGGCTTTTTAGGGGTCCGAGTAGGATGAAGCCGTTTAAATACAACCATCCCCAAGGGTTCTTCAGC CACCGCTGACTCCCCTCGACTTGTCATTCCCCAAAGCCCTGTCCTGGCTCCTGGGTATCTGCTTTTTGCT TTGGCCACATGTGGTTCTTGATATTTTCCAAAGACACCAGCTAATTAAAATGTCATCTGACAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3995737 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3995737 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003792 same experiment
	EMAGE:21583 same embryo
	EMAGE:21581 same embryo
	EMAGE:21585 same embryo
	EMAGE:21582 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source