EMAGE mouse gene expression spatial database

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EMAGE:21603

Arf2 ADP-ribosylation factor 2 ( MGI:99595)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002359. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002359_01	euxassay_002359_02	euxassay_002359_03	euxassay_002359_04

euxassay_002359_05	euxassay_002359_06	euxassay_002359_07	euxassay_002359_08	euxassay_002359_09

euxassay_002359_10	euxassay_002359_11	euxassay_002359_12	euxassay_002359_13	euxassay_002359_14

euxassay_002359_15	euxassay_002359_16	euxassay_002359_17	euxassay_002359_18	euxassay_002359_19

euxassay_002359_20	euxassay_002359_21	euxassay_002359_22	euxassay_002359_23	euxassay_002359_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21603_wholemount_strong.wlz

21603_wholemount_moderate.wlz

21603_wholemount_weak.wlz

21603_wholemount_possible.wlz

21603_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21603_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 1 weak expression: see section 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 2 3 4

spinal cord

weak

homogeneous

weak expression: see section 8 9 0 1 2 3 4 5

nasal septum

moderate

regional

moderate expression: see section 1 2 3 4 5

nose

moderate

regional

moderate expression: see section 7 8 9 0 6 7 8 9

basioccipital bone

moderate

regional

moderate expression: see section 1 2 3 4 8 9 0

basisphenoid bone

moderate

regional

moderate expression: see section 1 8 5 6

temporal bone

moderate

regional

moderate expression: see section 1 2 3 4 9 0

orbito-sphenoid

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 7 8 9 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3430
Entity Detected:	Arf2, ADP-ribosylation factor 2 ( MGI:99595)
Sequence:	sense strand is shown >T3430 GGGAATCAGTGACCTTTTTCATTAGAGTATCAAGACTGTAACACTAGAGAAGTTTCCAGCCTGCTCCAAA CGTGCCAAATGGAAACTCTGTGGGCTGCTGGCCCAGCAGGAGGCAGGACTCTTCCCTTAAGCCTCATGGT CCACGGTGGAGTGAAGGTTGGCTGGAGGAAGTGAAGGGAGGTAGCTCTTGTGTTCTGGCGTCATGGCTCA GGCCCTTGAGGCTGCTGCTCCCACTTCTCTGCTCTGTGCTGAGTGTTGTTCACATGAAGCAGACAGTTGT CCTGGGTGGAGTTGTCACTGTGTCCCGTTGTCCCAGTCTACTGTAGGCATTTGGTTAGTATCTGTTTCCA GAACTGCGCACCGTGAGTTCTGAGAGTTTGTGAGAAAGTCGGGAGGTTAATGTAGGAGATGATGTTCCCA GTGGCCCCGATACTCCCTGAGATGGTGCTAAACTGTGCTCCGAGAAGCATCTTTCACTTAGACTGCTCTC AAGGCATAGGCTCCCTCCTCAATGGCAGTGGCATCTGAAGAGTGGCTTGGAAGTGTGTGCTGTG
Notes:	The probe template was PCR amplified from IMAGE:3025910 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3025910 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002359 same experiment
	EMAGE:21600 same embryo
	EMAGE:21601 same embryo
	EMAGE:21602 same embryo
	EMAGE:21604 same embryo
	EMAGE:21605 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source