EMAGE mouse gene expression spatial database

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EMAGE:21653

Fem1a feminization 1 homolog a (C. elegans) ( MGI:1335089)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005657. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005657_01	euxassay_005657_02	euxassay_005657_03	euxassay_005657_04

euxassay_005657_05	euxassay_005657_06	euxassay_005657_07	euxassay_005657_08	euxassay_005657_09

euxassay_005657_10	euxassay_005657_11	euxassay_005657_12	euxassay_005657_13	euxassay_005657_14

euxassay_005657_15	euxassay_005657_16	euxassay_005657_17	euxassay_005657_18	euxassay_005657_19

euxassay_005657_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21653_wholemount_strong.wlz

21653_wholemount_moderate.wlz

21653_wholemount_weak.wlz

21653_wholemount_possible.wlz

21653_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21653_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5552
Entity Detected:	Fem1a, feminization 1 homolog a (C. elegans) ( MGI:1335089)
Sequence:	sense strand is shown >T5552 GACCCGCTAAGATGACAGCCCGGGAGAAGGTGAAGGTTGTCTCTGGCGACCGGCGGCCCGGCTAGATCCA GCAGCGAGGCTTCCAGCATGGATCTGCACACAGCGGTGTACAATGCGGCGCACGACGGCAAGCTGCCGCT GCTGCAGAAGCTGCTGGCCGGCCGTGGGCGTGAGGAGCTGGAGGAGCTGCTGGGTGAGGTGGCCGGCGGT GGCACGCCACTGCTAATCGCAGCGCGCCGTGGACACCTAGACGTGGTGGAGTATCTGGTGGACCACTGCG GCGCCAGCGTGGAGGCGAGCGGCTCCGTGCACTTCGATGGCGAGACCATCGAGGGCGCGCCGCCGCTCTG GGCAGCGTCGGCCGCGGGTCACCTGGCCGTGGTGCGTAGCCTTCTGCGCCGAGGCGCCTCGGTCAACCGC ACCACGCGCACCAACTCCACGCCGCTGCGCGCCGCCTGCTTCGACGGCCACCTGGACGTGGTACGCTACC TGGTGGGCGAGCACAAGGCGGACCTGGAGGTGGCCAATCGCCACGGCCACACGTGCCTTATGATCTCCTG CTACAAGGGCCACCGCGAGATCGCGCGCTACCTGCTGGAGCGCGGCGCACAGGTGAACCGGCGCAGCGCC AAGGGCAACACCGCCCTGCACGACTGTGCAGAATCTGGCAGCCTGGAGATCCTACAGCTGCTGCTGGGCT GCCACGCGCGCATGGAGCGCGACGGCTATGGCATGACCCCGCTGCTGGCCGCCAGCGTCACCGGGCACAC CAACATCGTGGAGTACCTCATCCAGGAACAGCCGGGCCACGAGCAGCTCTCAGGGACAGAGCTTCCCGGG GAAGGCTCCTCCCAGGTGGCAGGAAACCACTGCTCCACCCCAGAAGAAGCAGAACCGTATGAGAGCTGCT GCCCTACCAGCCGGGAAGCAGCTGTGGAAGCTTTGGAGCTGCTGGGTGCCACCTACGTGGATAAGAAAAG GGATCTGCTTGGAGCCCTGAAGCACTGGAGAAGGGCGATGGAACTCCGTCACCAGGGTGGAGGCTACCTC CCTAAGCCGGAGCCCCAGCAACTGGTTCTGGCCTATGACTATTCCAGGGAGGTGACTACACCCCAAGAGT TGGAAGCCCTCATTACAGATCCTGATGAAATGCGGATGCAGGCGCTGCTGATACGGGAGAGAATCTTGGG CCCCTCACATCCTGACACTTCATACTACATACGGTACCGGGGTGCTGTCTATGCGGACTCCGGGAATTTC GAGCGCTGTATCCGTCTGTGGAAGTATGCCTTGGACATGCAACAAAACAACTTGGAGCCCCTGAGCCCCA TGACCGCCAGCAGCTTCCTGTCGTTTGCAGAGCTCTTCTCCTACGTGCTGCAGGACCGCTCGGCCAAGGG CAACCTGGGCATGCAGCTTGGCTTTGCCGACCTCATGGGTGTGCTCAGCAAAGGGGTTCGGGAAGTGGAG CGGGCTCTGCAGTTGCCCAAGGAACCGGGTGACTCCGCACAGTTCACCAAAGCCATTGCCATCATCCTCC ACCTTCTGTACCTATTGGAGAAGGTGGAGTGCACCCCCAGCCAGGAACACCTCAAGCACCAGACAGTCTA CCGCCTGCTCAAGTGTGCCCCTCGCGGTAAAAACGGCTTCACCCCACTACACATGGCGGTGGACAAGGAG ACCACCAACGTGGGCCGGTATCGTGTGGGCGTCTTCCCATCGCTGCAGGTGGTCAAGGTGCTGTTGGACT GCGGAGCAGACCCTGACAGCAGGGATTTCGACAACAACACCCCACTGCACATCGCTGCCCAGAATAATTG CCCGGCCATCATGGATGCTCTCATCGAAGCTGGGGCCCACATGGACGCCACCAATGCCTTCAAAAAGACT GCCTACGAGCTGCTGGACTCGAAGCTGTTGGCTAAGAGCACCGTGCAGCCTTTCAATTATGTGACACTGC AGTGCCTAGCTGCCCGTGCCCTGGACAGGAACAAGGTCCCTTATAAGGGCTTCATCCCAGAGGAGCTGGA GGCCTTCATCCAGTTGCATTGAGCCTAGCCCAGCTGTTGGTGCCCACTTGGGCCTTACTCCTCCAAGCAG AAGCATCCAGGCCCTATGGCCTGCTGGAGTGAACCCAGCTGCTGATTCTAGAAGCCTTCAGGGTCACATG GTAGGAAGACGGTCTTTTTTTCTGTGGAAGTGCAAAAACAAAAACCCGGATGCCTGAGCCTCCCCAGTGG CATGCTTCTGTCTGGGTCTGGAGGCCTCTGCACTGTCCTAGAGTCCTGCAGTGAGCAAAGCTGTCTCCTT CCTGTGGTGGGCACTGACCACCTAGATGTTTGCATTTGGTTTTTCCTTTTCTAAGCAGATGGGCAGCAGC CTGGAGCCCATGTTGTGAGGGTTTTTCTGTCTGTTTTCACTGGTTTCTGTTTGTTGGACTTAGTAATGAC TGAGTTGAGAGTTGCCAGTATTTTGCAGTGGCCACAGCTGAGAACAGCATGAACTGTGTGTGCAGATCTG TGACCAAGATCCTGGGAAGCCTCCTCAGTTCTGTGCTGATTAGAGAGAGGACTATCTCCCAAGAAAGCCC AGAGCCAGTTGCAAGAGCCACAGCCAGGCAGACACTGGTGCCTTCTGTCCCGCCCTCTGGATGGACAGAC AGTTCCCAACCTTGGGACACAGCTGGAACTGCCAGGCAGATGGTGACCAGGCCACCTGGCCACCAGGTTC ACATCTGTGCCACACTTGTCACACTGTCACCTTCATCGCTGAGGACCTGACCCCTCTTTTGTTGAGGAGT CCCCGGTATCCCAATAACTTCTGGGGATGGGCTTCTAACCAAGATAGGGGAAGGTGGGGGGCTGCCAGCC AGGGTAGCGATGGACCCATGTGGTACTAGCTTGGCCCACGTGAGACCCTGTGTGCAGCCCCAGAACCACA AAAAGTTACTGAACAGCTGCCTATGACTCCACCAGCTCTCGATTCCATCATATGTGGTGAGTGGTTCACC CTGGATTAGGCCAAAGCAGGAGGTACTCATCCCCGGCCGTAGAAGCCAGCTACATGGATAGGAAGTCATG TGTAGAGGGGCCAGTCCCTCCCTCAGCCATTTAAGCCACATAAACTGTCCCTTGAGGACCGTCAGTGTGT GATTGCAGTGCTAGGTAGATTCTCTGTATAAGCAAACTTTAGGCCTTACTGGGCAGGGTCTCGCTGCTCC ACCCTGCCAAAGCTCAGGGCAAACATGAACCCCTTTGAGTCTTGGCACTGATATTTATGGATTAAAATGC AGGTATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:6469158 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6469158 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005657 same experiment
	EMAGE:21655 same embryo
	EMAGE:21654 same embryo
	EMAGE:21656 same embryo
	EMAGE:21657 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source